Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Single-cell proteomics takes centre stage

Deducing the full protein complement of individual cells has long played second fiddle to transcriptomics. That’s about to change.
  1. Jeffrey M. Perkel
    View author publications

    You can also search for this author in PubMed  Google Scholar

Ying Zhu places a chip containing samples for analysis into the automated nanoPOTS system.

Chemist Ying Zhu places a nanoPOTS chip containing protein samples into an automated analysis system. Credit: Andrea Starr/PNNL

Claudia Ctortecka was both sceptical and intrigued when her thesis adviser told her in 2018 about a new method that uses mass spectrometry to analyse the protein contents of individual cells. When he said he was looking for someone to pursue this single-cell proteomics strategy in his laboratory at the Vienna BioCenter research institute, she decided to take a chance.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

Nature 597, 580-582 (2021)

doi: https://doi.org/10.1038/d41586-021-02530-6

Updates & Corrections

  • Correction 27 September 2021: This Technology feature omitted the citation for Erwin Schoof’s study. It should have cited E. M. Schoof et al. Nature Commun. 12, 3341 (2021) as reference 6, with subsequent citations renumbered.

References

  1. Hartlmayr, D. et al. Preprint at bioRxiv https://doi.org/10.1101/2021.04.14.439828 (2021).

  2. Nagaraj, N. et al. Mol. Syst. Biol. 7, 548 (2011).

    Article  PubMed  Google Scholar 

  3. Zhu, Y. et al. Nature Commun. 9, 882 (2018).

    Article  PubMed  Google Scholar 

  4. Cong, Y. et al. Chem. Sci. 12, 1001–1006 (2021).

    Article  Google Scholar 

  5. Brunner, A.-D. et al. Preprint at bioRxiv https://doi.org/10.1101/2020.12.22.423933 (2021).

  6. Schoof, E. M. et al. Nature Commun. 12, 3341 (2021).

    Article  PubMed  Google Scholar 

  7. Woo, J. et al. Preprint at bioRxiv https://doi.org/10.1101/2021.02.17.431689 (2021).

  8. Budnik, B., Levy, E., Harmange, G. & Slavov, N. Genome Biol. 19, 161 (2018).

    Article  PubMed  Google Scholar 

  9. Li, J. et al. J. Proteome Res. 20, 2964–2972 (2021).

    Article  PubMed  Google Scholar 

  10. Leduc, A., Huffman, R. G. & Slavov, N. Preprint at bioRxiv https://doi.org/10.1101/2021.04.24.441211 (2021).

  11. Specht, H. et al. Genome Biol. 22, 50 (2021).

    Article  PubMed  Google Scholar 

  12. Ctortecka, C. et al. Preprint at bioRxiv https://doi.org/10.1101/2021.02.11.430601 (2021).

  13. Piehowski, P. D. et al. Nature Commun. 11, 8 (2020).

    Article  PubMed  Google Scholar 

  14. Guo, G. et al. Nature Commun. 12, 3241 (2021).

    Article  PubMed  Google Scholar 

  15. Mund, A. et al. Preprint at bioRxiv https://doi.org/10.1101/2021.01.25.427969 (2021).

  16. Smith, L. M. et al. Preprint at https://doi.org/10.20944/preprints202010.0368.v1 (2020).

Download references

Subjects

Latest on:

Nature Careers

Jobs

  • Director for the Wisconsin National Primate Research Center

    The University of Wisconsin-Madison seeks a Director for the Wisconsin National Primate Research Center (WNPRC).

    Madison, Wisconsin

    University of Wisconsin-Madison Office of Vice Chancellor for Research

  • Postdoctoral Fellow

    New Orleans, Louisiana

    Tulane University School of Medicine

  • Assistant Professor

    The Department of Human Genetics at the University of Michigan Medical School (Ann Arbor, Michigan) seeks to fill two tenure-track faculty position...

    Ann Arbor, Michigan

    University of Michigan - Human Genetics

  • Assistant Professor Tenure-Track Faculty Position

    UGA seeks vibrant candidates for tenure-track Assistant Professor position in the Center for Molecular Medicine (CMM). Attractive startup package.

    Athens, Georgia

    Center for Molecular Medicine at the University of Georgia

  • Research Associate (Clinical Research)

    Houston, Texas (US)

    Baylor College of Medicine (BCM)

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing

Search

Advanced search

Quick links