Letter to the Editor | Published:

Altered neurogenesis and disrupted expression of synaptic proteins in prefrontal cortex of SHANK3-deficient non-human primate

Cell Research volume 27, pages 12931297 (2017) | Download Citation

Dear Editor,

Despite substantial progress made toward understanding the molecular changes contributing to autism spectrum disorders (ASD), the neuropathophysiology underlying ASD remains poorly understood1,2. Structural brain imaging in humans is valuable, but lacks resolution at the cellular level. Studies of neuropathology in humans have been hampered by the lack of high quality postmortem brains from individuals with ASD2. For more than decades, mutant mice have served as major tools to dissect the pathophysiology of ASD because of the wealth of molecular and neurobiological techniques developed for studies with rodents. Our knowledge of molecular and cellular mechanisms for ASD is mostly limited to what we have learned from genetically modified mice. However, there are significant evolutionary differences in brain structure and behavior between rodents and humans. For example, social behaviors and the organization of cerebral cortex differ significantly between primates and rodents. The cerebral neocortex comprises 80% of the human brain and 72% of the macaque brain, but only 28% of the rat brain. Prefrontal cortex (PFC), a critical region for high order cognitive and social functions, is under-developed in rodents compared with primates. The unique behavioral features in human ASD have posed significant challenges to assess the translational value of many findings from ASD mouse models. The apparent evolutionary differences in brain structures and behaviors between mouse and human highlight the need of alternative animal models such as non-human primate models for ASD3. The value of a primate model to study ASD has been supported by the generation and characterization of monkeys with altered expressions of MECP2 using the lentivirus- and TALEN-based method4,5.

Mutations in human SHANK3 remain one of the best-replicated and well-characterized genetic defects in ASD. Various types of mutations in SHANK3 have been found in 1% of individuals with ASD6. SHANK3 protein is one of the three-member family proteins (SHANK1-3) known to function as postsynaptic scaffolding proteins in excitatory synapses. So far, a total of 14 independent lines of Shank3-mutant mice have been reported, but none of these studies focused on the neuropathology in the prenatal brain6,7. These mutant mice display a spectrum of behavioral phenotypes. However, interpretation of the behavioral phenotypes in rodent models relevant to the SHANK3-related disorders remains a challenge. For these reasons, SHANK3 represented a unique opportunity to test the feasibility of generating an ASD model in non-human primates.

We used the Cas9/sgRNA method to disrupt SHANK3 gene in cynomolgus monkeys (Macaca fascicularis) following previous protocols8,9. SHANK3 has an array of mRNA isoforms due to multiple promoters and extensive alternative splicing of coding exons in both human and rodent, and this pattern is expected to be conserved in non-human primates5,10. We therefore simultaneously targeted two sites (exons 6 and 12) of SHANK3 to induce a large deletion (Figure 1A and Supplementary information, Figure S1A) that could completely disrupt all SHANK3 isoforms. We selected sgRNAs that showed high targeting efficiency for injection into monkey embryos.

Figure 1
Figure 1

Generation and phenotypic analysis of SHANK3-mutant cynomolgus monkeys. (A) Schematic diagram of Macaca fascicularis SHANK3 gene, protein domains, Cas9/sgRNA targeting sites in exons 6 and 12, and confirmed mutations of SHANK3 in SHANK3M1-M3 animals. The gene structure of monkey SHANK3 was deduced from the mRNA of XM_015456961 and a genomic contig of NC_022281 for SHANK3. (B) Summary of embryo transplantation and surrogacy in cynomolgus monkeys. (C) Brain region-specific expression profile of SHANK3 protein in wild-type P0 monkey. SHANK3 displays isoform-specific expression in different brain regions and is expressed at the highest level in PFC. (D) Western blot analysis of SHANK3 protein in the PFC and striatum of SHANK3-mutant monkeys. Ctl1 and Ctl2 are age-matched controls at gestational day 135 and full-term gestation, respectively. (E) Altered expressions of postsynaptic receptors and scaffold proteins in SHANK3M1 brain. Normal expressions of GluN1, GluA2, Homer1b/c, and pan-Homer but decreased expressions of GluN2B, mGluR5, and PSD95 were observed in the PFC of SHANK3M1. (F) Western blot analysis of NeuN, GFAP, and DCX in whole-cell lysates from PFC. SHANK3M1 showed decreased levels of NeuN and DCX but increased level of GFAP in the PFC. Western blot analysis was repeated at least three times independently. (G) NeuN and GFAP staining (brown) in layers I-VI of PFC. NeuN staining showed a strong signal in the nucleus and a weak signal in the cytosol. Nuclei were stained with hematoxylin (blue). There were fewer NeuN+ neurons and more GFAP+ astrocytes in the PFC of SHANK3M1. Scale bar, 100 μm. (H) Percentages of NeuN+ neurons and GFAP+ astrocytes in each layers of PFC. n = 6, *P < 0.05, **P < 0.01, and ***P < 0.001 by Student's t-test.

We obtained 3 pregnancies after transferring 116 injected embryos to 37 surrogate mothers (Figure 1B). The pregnancy rate of 8.1% (3/37) was significantly lower than that of other targeted genes (34.5%) performed in the same center under the same experimental conditions (Li X et al., unpublished data). The reason for the low pregnancy rate is currently unknown, but suggests that SHANK3 expression might be important for early development in primates. Of three monkey offsprings, one miscarried close to the term gestation (125 days) (SHANK3M1), one was lost due to a difficult labor (SHANK3M2), and one is still alive (SHANK3M3) (Supplementary information, Figure S1B). There are no apparent developmental defects in SHANK3M1 and SHANK3M2 fetuses to explain their death. The brain and other somatic tissues of SHANK3M1 and SHANK3M2, and selected somatic tissues of SHANK3M3 were analyzed for targeted mutations at sites 1 and 2. All three offsprings were positive for mutations at site 2 of exon 12 (−22 and −8 bp in SHANK3M1, −5 and +1 bp in SHANK3M2, and −2 bp in SHANK3M3; Supplementary information, Figure S1C–S1F). A large deletion was detected in different tissues of SHANK3M1 with a pair of primers spanning sites 1 and 2, as evidenced by a positive band around 500 bp (Supplementary information, Figure S1C). Sequencing of this band confirmed an 11 456 bp deletion between the two targeting sites (Supplementary information, Figure S1E). The small indels in different mutant animals were confirmed by deep amplicon sequencing (Supplementary information, Figure S1F). However, deep amplicon sequencing was unable to detect the large 11 456 bp deletion. Using the semi-quantitative PCR analysis of genomic DNA, we estimated that 70% of the alleles carry the large deletion in SHANK3M1, which is predicted to disrupt the expression of most SHANK3 isoforms based on the transcript pattern of SHANK3 in human and rodent6,10. Off-target analysis did not reveal any additional mutations (Supplementary information, Figure S1G).

The brain tissues from SHANK3M1 and SHANK3M2 provided a unique opportunity to investigate SHANK3 mutation-associated neuropathology. Shank3 displays a temporal- and spatial-specific expression for its isoforms in mouse brain with peak expression in striatum of postnatal day 14 (P14) brain (Supplementary information, Figure S2). In contrast, we found that SHANK3 showed the highest level of expression in the PFC of monkey at birth (P0), a developmental time point that corresponds to P14 in mice (Figure 1C). The distinct expression patterns of SHANK3 in mouse and monkey brains may reflect the evolutionary differences between the two species.

We performed western blot analysis using whole-cell lysates isolated from different brain regions of mutants and age-matched controls (Ctl1 for SHANK3M1 and Ctl2 for SHANK3M2) using an antibody against the C-terminus of mouse Shank3 that is predicted to detect most, if not all, SHANK3 isoforms of primates (Figure 1D)11. Compared to the control, no SHANK3 protein isoforms were detected in the cortex of SHANK3M1 animal, indicating a complete loss of SHANK3 resulting from the 11 kb, 22 and 8 bp deletions in the PFC (Figure 1D). In contrast, an isoform-specific deficiency of SHANK3 was revealed in the striatum of SHANK3M1 animal, in which the full-length isoform was absent but a short isoform was still present. In the SHANK3M2 brain, the full-length SHANK3 was decreased to a greater extent than the short isoform in the striatum. Interestingly, the majority of isoforms were not decreased but mildly increased in the cortex presumably by a compensatory mechanism. These findings are generally consistent with the mosaic nature of CRISPR/Cas9-mediated gene editing, which accounts for the various extents of disruption of the targeted gene in different brain regions. However, the isoform-specific expression of SHANK3 in different brain regions should also be considered.

Given the alteration of SHANK3-interacting proteins in Shank3-deficient mice6,7, we selected several synaptic proteins for western blot analysis in SHANK3-deficient monkey brain (Figure 1E, Supplementary information, Figure S3A and S3B). We found no apparent difference in the levels of GluN1, GluA2, Homer1b/c, and pan-Homer in the PFC between SHANK3M1 and control. However, a significant decrease was revealed for mGluR5 (54.7% ± 10.6% of ctl1, n = 5, mean ± SEM), GluN2B (18% ± 6.8%, n = 3), and PSD95 (15.5% ± 2.6%, n = 6) in the PFC of SHANK3M1 compared to the control. In striatal neurons of Shank3-deficient mice, Homer1b/c abnormally accumulates in the cytoplasm despite no significant increase in overall proteins11. Consistently, more cytoplasmic staining of Homer1b/c was also observed in PFC neurons of SHANK3M1 animal compared with its control (Supplementary information, Figure S4A and S4B). We further co-stained SHANK3 and PSD95, and found apparent reductions in the staining intensity of the two proteins in the PFC of SHANK3M1 (Supplementary information, Figure S5A), consistent with the western blot results (Figure 1D and 1E). The loss of SHANK3 and alterations in its interacting proteins in SHANK3M1 brain verify the findings from other species11,12.

The abundant expression of SHANK3 in the control but a complete loss of SHANK3 in the PFC of the SHANK3M1 animal led us to focus on the pathological outcome in this brain region. Immunohistochemical analysis revealed that the structure of cortical layers is comparable between SHANK3M1 and its control. While the cell density of cortical layers did not differ between control and mutant PFCs, the cell bodies of SHANK3M1 were smaller (Figure 1G). Strikingly, we found significantly fewer NeuN+ neurons in the PFC of SHANK3M1 (72.2% ± 1.2% NeuN+ cells/total cell counts for Ctl1 and 22.7% ± 5% for SHANK3M1; Figure 1G and 1H) with reduced soma size (48.0 ± 10.2 μm2 NeuN+ cell for Ctl1 and 22.7 ± 4.8 μm2 for SHANK3M1, n = 6 slices). On the contrary, there were significantly more GFAP+ astrocytes in the PFC of SHANK3M1 animal compared to the control (Figure 1G and 1H) based on quantitative analysis of DAB staining results. Double immunofluorescent staining confirmed fewer NeuN+ cells in the PFC and the increased GFAP-labeling in the PFC and striatum of SHANK3M1 brain (Supplementary information, Figure S4C and S4D). The histological studies of SHANK3M2 did not reveal any apparent abnormalities in the PFC (Supplementary information, Figure S5B), consistent with the finding of no apparent loss of SHANK3 in SHANK3M2 brain. The fewer NeuN+ neurons and more GFAP+ astrocytes in the PFC of SHANK3M1 were further supported by western blot analysis (Figure 1F). Specifically, the NeuN protein was markedly reduced (25% ± 8.7%, n = 5), whereas the GFAP protein was significantly increased (357% ± 144%, n = 5) in SHANK3M1 PFC compared to control (Supplementary information, Figure S3C). Western blot analysis also showed a decreased level of Doublecortin (DCX; 65% ± 3.7%, n = 3), a marker of progenitor neurons, in the PFC, but a normal level in the striatum of SHANK3M1 brain (Figure 1F and Supplementary information, Figure S3C). These results together suggest a brain region-specific neurogenesis defect in SHANK3M1 mutants.

Given the altered synaptic receptors and PSD proteins in SHANK3M1 brain, we used Golgi impregnation method to examine dendritic spines in the PFC of SHANK3M1 and SHANK3M2 brains and observed a significant decrease in dendritic spine density of the SHANK3M1 but not SHANK3M2 mutants compared to control, whereas the length from the tip to the base of a spine was not significantly altered in both mutant animals (Supplementary information, Figure S6). These results are consistent with previous reports in Shank3-mutant mice or neurons derived from SHANK3-deficient patients documenting a reduced spine density11,12.

In summary, we successfully introduced various types of deleterious mutations in SHANK3 for the first time in cynomolgus monkeys by the CRISPR/Cas9 genome editing method. SHANK3-deficient fetus showed a significant loss of NeuN+ neuronal cells accompanied with an increase of GFAP+ astrocytes, which has not been found in any line of Shank3-knockout mice, demonstrating a unique and critical role of SHANK3 in early brain development in primates. SHANK3 has been known as a scaffolding protein in synapses. However, our findings suggested an important role of SHANK3 in early developing brains in non-human primate. The mechanism by which SHANK3 regulates neurogenesis, in addition to the conventional synaptic function, in the prenatal brain remains to be elucidated. Our findings from SHANK3-deficient monkey predict that similar but milder changes may underlie the pathophysiology of human individuals with SHANK3 haploinsufficiency. The defects in early brain development and the reduced size of neuron soma are reminiscent of the neuronal defects in MeCP2 and PTEN ASD mouse models1. The pattern of molecular changes resulting from the SHANK3 deficiency is overall conserved between mouse and monkey brains, but the pathological changes are more prominent in SHANK3-deficient monkey. While these findings support the value of mouse models, they also underscore the importance of using non-human primates to model SHANK3 and other gene mutations causing ASD.

Materials and Methods are available in Supplementary information, Data S1, Tables S1 and S2.

References

  1. 1.

    , , , et al. Nat Med 2016; 22:345–361.

  2. 2.

    , , . Trends Neurosci 2008; 31:137–145.

  3. 3.

    , . J Neurodev Disord 2012; 4:21.

  4. 4.

    , , , et al. Cell 2017; 169:945–955.

  5. 5.

    , , , et al. Nature 2016; 530:98–102.

  6. 6.

    , . Neuron 2013; 78:8–27.

  7. 7.

    , . Nat Rev Neurosci 2017; 18:147–157.

  8. 8.

    , , , et al. Sci Rep 2017; 7:42081.

  9. 9.

    , , , et al. Cell 2014; 156:836–843.

  10. 10.

    , , , et al. Hum Mol Genet 2014; 23:1563–1578.

  11. 11.

    , , , et al. Nat Commun 2016; 7: 11459.

  12. 12.

    , , , et al. Nature 2013; 503:267–271.

Download references

Acknowledgements

We thank Drs Weixiang Guo, Xiaoming Wang and Zhiheng Xu for discussion, Ruxiao Xing, Xudong Liu and Jinquan Gao for technical assistance, Yafeng Luo (Guangzhou Yuanxi Biotech Co., Ltd) for animal resource and care, and Samuel Hulbert for critical reading of the manuscript. The project was supported by the Ministry of Science and Technology of China (2014CB942803 and 2016YFA0501000), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02020400), and the National Natural Science Foundation of China (31110103907, 91332206 and 31490592).

Author information

Author notes

    • Hui Zhao
    •  & Zhuchi Tu

    These two authors contributed equally to this work.

Affiliations

  1. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

    • Hui Zhao
    • , Zhuchi Tu
    • , Huijuan Xu
    • , Yinghui Zheng
    • , Rui Tian
    • , Xiao-Jiang Li
    •  & Yong Q Zhang
  2. University of Chinese Academy of Sciences, Beijing 100101, China

    • Hui Zhao
    •  & Yong Q Zhang
  3. Guangdong-Hongkong-Macau Institute of CNS Regeneration, Ministry of Education CNS Regeneration Collaborative Joint Laboratory, Jinan University, Guangzhou, Guangdong 510632, China

    • Zhuchi Tu
    • , Sen Yan
    • , Weili Yang
    • , Xiangyu Guo
    •  & Xiao-Jiang Li
  4. The Institute for Brain Research, Collaborative Innovation Center for Brain Science, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China

    • Huanhuan Yan
    •  & Youming Lu
  5. Yuanxi Biotech Inc., Guangzhou, Guangdong 5010663, China

    • Jiezhao Zheng
    •  & Zhujun Li
  6. Department of Pediatrics and Department of Neurobiology, Duke University, Durham, NC 27710, USA

    • Yong-hui Jiang
  7. Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA

    • Xiao-Jiang Li

Authors

  1. Search for Hui Zhao in:

  2. Search for Zhuchi Tu in:

  3. Search for Huijuan Xu in:

  4. Search for Sen Yan in:

  5. Search for Huanhuan Yan in:

  6. Search for Yinghui Zheng in:

  7. Search for Weili Yang in:

  8. Search for Jiezhao Zheng in:

  9. Search for Zhujun Li in:

  10. Search for Rui Tian in:

  11. Search for Youming Lu in:

  12. Search for Xiangyu Guo in:

  13. Search for Yong-hui Jiang in:

  14. Search for Xiao-Jiang Li in:

  15. Search for Yong Q Zhang in:

Corresponding authors

Correspondence to Yong-hui Jiang or Xiao-Jiang Li or Yong Q Zhang.

Supplementary information

PDF files

  1. 1.

    Supplementary information, Figure S1

    Generation of SHANK3 gene (NCBI Reference, NW_012011200.1) mutant monkeys.

  2. 2.

    Supplementary information, Data S1

    Materials and Methods

  3. 3.

    Supplementary information, Tables S1 and S2

    The off-target analysis and mosaic analysis of mutant offspring

About this article

Publication history

Published

DOI

https://doi.org/10.1038/cr.2017.95

(Supplementary information is linked to the online version of the paper on the Cell Research website.)

Further reading