Spermatogenesis in Kitw/Kitwv mouse testis after PGCLC transplantation. (A) A single seminiferous tubule transplanted with the PGCLCs, showing (white arrow head) or not showing (yellow arrow head) spermatogenesis. (B) Spermatozoa derived from the PGCLCs. Scale bar = 20 μm. (C) Sections of the PGCLC-transplanted testes after H&E staining. Scale bar = 100 μm. (D) Immunostaining of the PGCLC-transplanted testes with anti-Vasa (red) and PNA (green). Hoechst 33342 (blue) stained the DNA. Scale bar = 100 μm. (E) One live offspring generated by ICSI using W-1R spermatozoa. (F) DNA sequencing of W and WV point mutations of the offspring shown in E.