The piRNA machinery is known for its role in mediating epigenetic silencing of transposons. Recent studies suggest that this function also involves piRNA-guided cleavage of transposon-derived transcripts. As many piRNAs also appear to have the capacity to target diverse mRNAs, this raises the intriguing possibility that piRNAs may act extensively as siRNAs to degrade specific mRNAs. To directly test this hypothesis, we compared mouse PIWI (MIWI)-associated piRNAs with experimentally identified cleaved mRNA fragments from mouse testes, and observed cleavage sites that predominantly occur at position 10 from the 5′ end of putative targeting piRNAs. We also noted strong biases for U and A residues at nucleotide positions 1 and 10, respectively, in both piRNAs and mRNA fragments, features that resemble the pattern of piRNA amplification by the 'ping-pong' cycle. Through mapping of MIWI-RNA interactions by CLIP-seq and gene expression profiling, we found that many potential piRNA-targeted mRNAs directly interact with MIWI and show elevated expression levels in the testes of Miwi catalytic mutant mice. Reporter-based assays further revealed the importance of base pairing between piRNAs and mRNA targets and the requirement for both the slicer activity and piRNA-loading ability of MIWI in piRNA-mediated target repression. Importantly, we demonstrated that proper turnover of certain key piRNA targets is essential for sperm formation. Together, these findings reveal the siRNA-like function of the piRNA machinery in mouse testes and its central requirement for male germ cell development and maturation.
The PIWI proteins are a subfamily of the AGO/PIWI proteins that are mainly expressed in animal germ cells1. A class of 24-31-nt PIWI-binding small noncoding RNAs (ncRNAs), termed as PIWI-interacting RNAs (piRNAs), have been identified in mammalian testes2,3,4,5. This class of small ncRNAs is slightly longer than microRNAs (miRNAs) and small interfering RNAs (siRNAs), exhibits great sequence variety and generally is mapped to clustered loci in the genome. It has been proposed that the biogenesis of piRNAs includes a primary processing pathway and a 'ping-pong' amplification cycle pathway6,7,8. Increasing evidence suggests that piRNAs play a critical role in both epigenetic and post-transcriptional silencing of transposons1,6. Several studies indicate that piRNAs in Drosophila have the capacity to downregulate the expression of certain mRNA targets via a miRNA-like mechanism that operates by imperfect base-pairing rules9,10,11,12,13. However, it is not yet known whether and to what extent this mechanism is involved in regulating gene expression in mammals.
In mice, PIWI proteins consist of three members: MILI, MIWI and MIWI2. The expression of Mili, Miwi and Miwi2 during male germ cell differentiation is temporally and spatially regulated, which is essential for spermatogenesis. Interestingly, mouse piRNAs are expressed in two distinct phases during spermatogenesis, termed as pre-pachytene and pachytene piRNAs, respectively. Pre-pachytene piRNAs are mainly derived from transposons and are coexpressed with MIWI2 and/or MILI in fetal and perinatal male germ cells, and function in transposon silencing, thereby protecting the genome in early-stage spermatogenic cells14,15,16. Pachytene piRNAs, on the other hand, are massively produced from non-transposon intergenic regions in later pachytene spermatocytes and post-meiotic spermatids, and are mainly associated with MIWI. However, little is known about the functional significance of MIWI and the MIWI-bound pachytene piRNAs.
A recent study indicates that the slicer activity of MIWI is responsible for cleavage of transposon transcripts in mouse testes17. However, it has remained unclear whether MIWI exerts a similar piRNA-guided endonuclease activity on piRNA-targeted mRNA transcripts. In this study, we analyzed the published global 5′-RACE libraries from mouse testes for cleaved RNA fragments that contain a 5′-monophosphate, a structural feature likely to be generated by certain RNA slicer activity17. We identified approximately 200 potential piRNA target sites on specific mRNAs, thus implicating piRNAs in mediating cleavage of these mRNAs in mouse male germ cells. Following this initial finding, we performed anti-MIWI crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq) on enriched mouse round spermatids and demonstrated that ∼43% of the identified piRNA target sites were physically associated with MIWI. Subsequently, we validated the MIWI/piRNA-mediated repression of target mRNAs with reporter-based assays and showed that both the slicer activity and the piRNA-loading ability of MIWI are critical for the piRNA-mediated target repression. Importantly, ectopic expression of piRNA-resistant forms of target genes in spermatids prevented the formation of sperms, suggesting that proper turnover of certain piRNA targets is essential for normal spermiogenesis. Together, these results reveal a pachytene piRNA-directed mRNA cleavage program that is critical for mouse male germ cell development.
Identification of potential MIWI-cleaved mRNA fragments
The slicer activity of PIWI proteins is required for piRNA amplification by the 'ping-pong' mechanism and post-transcriptional silencing of transposons7,8,17,18. Taking advantage of 5′-RACE cleavage fragment libraries derived from adult testes of both control (Miwi+/−) and MIWI slicer activity-deficient mutant (Miwi−/ADH) mice17, we selected fragments that show perfect matches with the sense strands of RefSeq mRNAs and considered these as potential MIWI-cleaved products. To ensure that all selected fragments are derived only from mRNAs, we only included fragments that correspond to unique loci in the mouse genome. Together with a library of MIWI-associated piRNAs from wild-type adult mouse testes17, we noted a positional relationship between potential piRNA-binding sites and a subset of mRNA fragments. As illustrated on Psma8, BC026590 and Mdc1, the putative cleavage sites appear to overlap with potential piRNA-binding sites with the cleavage position precisely located 10 nt from the 5′ end of the matching piRNAs (Figure 1 and Supplementary information, Figure S1).
The Psma8 mRNA fragments are mainly derived from its 3′-UTR and represent the most abundant cleavage products (645 reads per million (RPM)) in Miwi+/− testes (Figure 1A, top). Interestingly, the 3′-UTR of Psma8 appears to harbor potential target sites for several piRNAs (∼280 reads), and importantly, the 5′ ends of ∼96% of these piRNAs lay 10 nt downstream of the putative mRNA cleavage site (Figure 1A and 1B). Consistent with these mRNA fragments being piRNA-mediated cleavage products, their abundance was drastically reduced in the library derived from Miwi−/ADH testes (Figure 1A, bottom), and the Psma8 mRNA level was indeed elevated in such mutant testes (Figure 1C). We similarly identified multiple mRNA fragments from the 5′-RACE library, which overlap with potential piRNA-binding sites on the BC026590 (Figure 1D and 1E) and Mdc1 mRNAs (Supplementary information, Figure S1A and S1B), and observed elevated expression of these mRNAs in Miwi−/ADH mutant testes (Figures 1F and Supplementary information, Figure S1C). These data strongly suggest that MIWI is involved in piRNA-guided mRNA cleavage in mouse testes.
Widespread MIWI/piRNA-mediated mRNA cleavage events in mouse testes
The above examples of potential MIWI/piRNA-cleaved mRNA fragments prompted us to ask how common such cases exist in mouse testes. To this end, we systematically screened potential mRNA targets for piRNAs expressed in the mouse testes. Given that perfect or near-perfect base pairing between piRNA and mRNA is required for efficient cleavage by MIWI17, we first searched for base pairing with less than 3 mismatches between the piRNA from position 2 to 21 and the mRNA (see the base-pairing rule for piRNA targeting below). Next, we examined the distribution of potential piRNA-binding sites near the 5′ end of mRNA fragments identified in the 5′-RACE library. Consistent with the previous observation that MIWI cuts targets at the position 10 nt away from the 5′ end of targeting piRNAs17, we observed a strong enrichment of matching piRNAs whose 5′ ends fall in the position 10 nt downstream of the cleavage site (Figure 2A). In contrast, such enrichment was absent among mRNA fragments derived from the Miwi−/ADH mouse testes (Figure 2B), suggesting that the slicer activity of MIWI is essential for the production of such mRNA fragments. We obtained similar results if we only counted mRNA fragments instead of the targeting piRNAs (Supplementary information, Figure S2A). Altogether, we identified a total of 1 878 piRNA:mRNA interactions based on the peak at position 10 in Miwi+/− mouse testes (Figure 2A, Table 1 and Supplementary information, Table S1), which involves 1 295 non-redundant piRNAs and 193 mRNA fragments. We regard these 193 fragments residing in 169 specific protein-coding genes as potential piRNA target sites.
The above results strongly implicate an essential role of MIWI slicer activity in the regulation of mRNA stability beyond the two specific mRNAs shown in Figure 1. To further substantiate this, we compared the abundance of mRNA fragments with 5′ ends at the predicted piRNA-guided cleavage sites in Miwi+/− vs Miwi−/ADH 5′-RACE libraries17. We found that the reads of such mRNA fragments were sharply reduced in Miwi−/ADH mice (Figure 2C). Moreover, ∼94% of the predicted piRNA target sites were absent at the 5′ ends of mRNA fragments in the 5′ RACE library from Miwi−/ADH mice, supporting an essential role of MIWI slicer activity in generating mRNA fragments cleaved at such piRNA target sites. We further compared the expression levels of the predicted piRNA target genes between Miwi+/− and Miwi−/ADH testes, and found that the levels of these mRNAs were significantly upregulated relative to other non-target genes in the Miwi catalytic mutant (Figure 2D and Supplementary information, Figure S2B). Gene Set Enrichment Analysis (GSEA)19 also showed significant upregulation (P < 0.001) of the predicted target gene set in the catalytic mutant (Supplementary information, Figure S3). Collectively, these results suggest that piRNAs may regulate their target mRNAs via a siRNA-like mechanism in mouse testes.
Analysis of MIWI-mediated regulation of mRNA cleavage by CLIP-seq
To provide experimental evidence for MIWI/piRNA-mediated regulation of mRNA cleavage in mouse testes, we performed MIWI CLIP-seq, as we recently described20, to determine the physical interactions between MIWI and RNAs. Considering our previous results showing abundant MIWI and its associated piRNAs in round spermatids21, we performed MIWI CLIP-seq on round spermatids isolated from adult mouse testes (Supplementary information, Figure S4A). We identified ∼48 million reads that could be mapped to the mouse genome. Considering reads with 25-33 nt in length as piRNAs and longer reads (sequenced up to 36 nt) as MIWI-bound target RNAs (Figure 3A), we found that a total of ∼17 million reads correspond to piRNAs and ∼10 million reads correspond to MIWI targets. Importantly, while the majority of MIWI-bound piRNAs and targets are derived from various repeat-containing transcripts and long noncoding RNAs (lncRNAs), a substantial fraction of reads are also mapped to the sense strand of protein-coding genes (Figure 3B).
Interestingly, of 193 predicted piRNA target sites in 169 genes shown in Table 1, 164 target sites in 147 genes could be matched with MIWI CLIP-identified piRNAs at the expected cleavage position. Fragments of almost all target genes (168/169) were detected by MIWI CLIP-seq assay. Among these 168 genes, 83 predicted piRNA-target sites in 73 genes fall within 15 nt from the 5′-RACE-mapped cleavage sites. Under this strict condition, the predicted piRNA target sites on 65 of these 73 genes were supported by both MIWI CLIP-identified piRNAs and mRNA targets (Figure 3C). Using the mean coverage rate of CLIP-identified mRNA targets (including the flanking 15 nt) as the expected probability to perform a binomial test, we found that the coincidence of 193 predicted piRNA target sites relative to CLIP-identified targets within 169 genes was highly significant (P = 0.00018).
We noted that the expression levels of the predicted piRNA target genes that also show MIWI binding evidence were higher than those of other predicted target genes (t-test, P = 0.0028; Supplementary information, Figure S4B), indicating that CLIP-seq tends to catch target genes with higher expression levels. We also observed that the putative cleavage sites predicted from the 5-RACE library exhibited the highest coincidence with the 5′ ends of MIWI CLIP-identified mRNA fragments (Figure 3D), indicating that the cleaved products are physically associated with MIWI. Similarly to the observation depicted in Figure 2A, we also observed a significant enrichment of piRNAs (obtained from the Miwi+/+ dataset GSM82276017) whose 5′ ends match the position 10 from the 5′ ends of CLIP-identified mRNA fragments, with ∼500 genes presented in the peak at position 10, suggesting that the number of piRNA target genes may be more than that predicted by the 5′-RACE library (Supplementary information, Figure S4C). It is worth noting that compared with the results obtained from the 5′-RACE library (Figure 2A), the CLIP data showed higher background noise, which is likely contributed by MIWI-bound, but uncleaved mRNAs.
Validation of piRNA regulation of mRNA expression
Previous studies showed specific expression of Miwi during the developmental transition from meiotic spermatocytes to elongating spermatids and spermatogenic arrest in round spermatids in Miwi-null mice22. To experimentally determine the regulation of mRNA expression by MIWI and piRNAs, we selected 7 (Atr, BC026590, Gfpt1, Mdc1, Ppp1cb, Psma8 and Tox4) from the 169 predicted target genes for quantitative analysis. Among these genes, four (Atr, BC026590, Mdc1 and Ppp1cb) contain both MIWI CLIP-supported targeting piRNAs and target sites in their mRNAs. We isolated round spermatids (RS) and spermatocytes (SC) from adult Miwi+/− and Miwi−/− testes by unit gravity sedimentation as we recently described21, and determined the expression of these 7 target genes by RT-qPCR. We found that all the 7 tested genes were expressed at significantly elevated levels in round spermatids from Miwi−/− compared to Miwi+/− mice, with the largest differences observed for BC026590 and Psma8 (Figure 4A and Supplementary information, Figure S5A). The data thus support a direct role of MIWI in the regulation of mRNA stability in mouse testes.
We next performed reporter-based assays to determine the requirement for specific piRNA:mRNA base-pairing interactions in MIWI-dependent regulation of mRNA stability. We selected 3 piRNA:mRNA pairs (Psma8, BC026590 and Tox4) for the analysis and placed the predicted target sequences in the 3′-UTR of the luciferase reporter. We tested two controls for each reporter, one with an 8-nt deletion in the 5′ portion of piRNA-targeting region and the other with an 8-nt mutation at the 5′ end of piRNAs (Supplementary information, Figure S5B). A mouse spermatocyte cell line GC-2spd(ts)23, which we recently showed to be a suitable in vitro model for probing piRNA functions20, was used for reporter assays. Intriguingly, we found that the selected piRNAs for reporter assays (piR-mmu-7775524, piR-671 and piR-125394) were all absent in GC-2spd(ts) cells, despite the presence of piRNA and Miwi expression in these cells (NCBI-SRA: SRR1482465)20. When the reporters were co-transfected along with the cognate chemically synthesized piRNAs (containing a phosphate at the 5′ end and a 2′-O-methyl group at the 3′ end) into GC-2spd(ts) cells, we found that all wild-type reporters, but not their mutant versions, were significantly repressed by co-transfected wild-type, but not mutant, piRNAs (Figure 4B and Supplementary information, Figure S5C). RT-qPCR confirmed piRNA-dependent downregulation of the reporter mRNA in each case (Figure 4C and Supplementary information, Figure S5D).
We next examined the requirement for the MIWI protein in piRNA-induced target repression. To this end, we knocked down endogenous Miwi in GC-2spd(ts) cells by siRNAs. As expected, depletion of Miwi strongly attenuated the effect of piR-mmu-77755 on the BC026590 reporter activity (Figure 4D). We further examined the requirement for the MIWI slicer activity in piRNA-mediated target repression by complementing Miwi siRNA-treated GC-2spd(ts) cells with human PIWI (HIWI), which is highly homologous (∼94% identity) to MIWI22, or its slicer activity-deficient ADH mutant17. We found that, while wild-type HIWI was able to restore the piRNA function in Miwi-depleted GC-2spd(ts) cells, the ADH mutant HIWI failed to do so (Figure 4D). We also tested another mutant HIWI (Y345/346A) known to be defective in piRNA loading21, and found that the ability to load piRNA is also essential for piRNA-dependent, HIWI-mediated repression of the reporter (Figure 4D). These data unequivocally demonstrate that both the slicer activity and the piRNA-loading ability of MIWI/HIWI are indispensable for piRNA-induced target repression.
Rules for piRNA binding to target mRNA
Previous in vitro assays suggest that the MIWI catalytic activity requires continuous complementarity of ≥ 20 nt between piRNA and target17. However, both our computational analyses and reporter assays (Figure 4 and Supplementary information, Table S1 and Figure S5) suggest that a somewhat less strict base-pairing rule might suffice for MIWI/piRNA-mediated degradation of mRNA targets in the cell. We thus further explored the rules that govern piRNA-guided cleavage of mRNAs.
1. Length of complementarity We first determined the minimal continuous base-pairing length, starting from the second nucleotide of piRNA, and found that the enrichment for complementary piRNAs at the position 10 from the 5′ end of mRNA cleavage sites persistently remained under the perfect match (PM) of 16-22 nt in length (PM16-PM22; Supplementary information, Figure S6A). To estimate the false positive rate, we calculated the signal:noise ratio, in which the signal represents the peak value of piRNA counts at position 10, and the background noise represents average of values at positions ± 5 nt from position 10 (not including the position 10). Signal:noise ratios considerably above 1 were obtained with complementarity lengths as short as 16 base pairs (Supplementary information, Figure S6B). In sharp contrast, no significant enrichment was observed with the expressed piRNAs and mapped mRNA fragments from Miwi−/ADH testes, even when we only counted piRNAs with 20 continuous base pairs (Supplementary information, Figure S6C). These results suggest that MIWI-mediated target cleavage can be achieved with shorter continuous base pairing between piRNAs and their targets than previously thought.
2. Allowable mismatches We next tested the effect of mismatches (allowing 1-3 mismatches) in a continuous sequence of 20 nt starting from position 2 on piRNAs. Intriguingly, we still observed a significant enrichment of piRNAs at position 10 from the cleavage sites, even with 3 mismatches (Supplementary information, Figure S6D). However, as the number of mismatches increased, the signal:noise ratio was dramatically reduced (Supplementary information, Figure S6E), indicating that piRNAs with more than 3 mismatches are less likely to induce target cleavage.
3. Base-pairing potential from the 5' end of piRNA Previous studies indicate that mismatches at the first base of the piRNA do not affect piRNA-guided target cleavage by MIWI17. To further examine positional effects of mismatches, we examined piRNA-binding events around the mRNA cleavage sites with progressively increased mismatches from the 5′ end of piRNAs and < 3 mismatches in the subsequent 19-nt sequences. We obtained the highest piRNA-binding rate with paired base beginning at position 1, indicating that the first base-pair interaction is important for piRNA-mediated mRNA cleavage. We continued to detect an appreciable peak when paired base begins at position 2 or 3, but no peak was observed when paired base starts at position 4 or beyond (Supplementary information, Figure S6F and S6G).
These results suggest a general rule for predicting piRNA targeting regions on mRNAs, which requires a relatively strict base pairing at the 5′ end of piRNA with mismatches of < 3 in the following 20-nt sequence. Notably, the mRNA cleavage site at position 10 did not shift to a further downstream position when base pairing starts at position 2 or 3, indicating that the cleavage site is still present between the 10 and 11 nt relative to the piRNA 5′ end, rather than counting from the start base-pairing position between piRNAs and mRNAs.
Features of piRNA target sites
We further analyzed the 193 predicted piRNA target sites (Supplementary information, Table S1) and found that most of these sites (∼75%) were targeted by more than one piRNA (Figure 5A). Intriguingly, we found that more than 70% of these potential target sites were located in the 3′-UTRs (Figure 5B), despite hat 3′-UTR sequences only account for ∼30% of all mRNA fragments in the 5′-RACE library, suggesting that piRNA target sites are preferentially located in the 3′-UTRs of target mRNAs.
We further examined the conservation of potential piRNA target sites among placental mammals25, and found that the sequences around piRNA target sites are more conserved than surrounding sequences (Figure 5C), indicating that such piRNA-mediated regulation of mRNA stability might be an evolutionarily conserved mechanism in placental mammals.
Features of mRNA-targeting piRNAs
We next analyzed 1 295 predicted mRNA-targeting piRNAs (Supplementary information, Table S1) and found that these piRNAs range from 28 to 32 nt in length (Supplementary information, Figure S7A), consistent with the established preference of MIWI for piRNAs of ∼30 nt2,3. We observed that ∼60% of these piRNAs map to one unique locus (Supplementary information, Figure S7B), while a small number of these piRNAs map to more than 100 loci (some even map to > 1 000 loci) in the mouse genome. Moreover, we found that the majority of these 1 295 piRNAs originate from loci that are annotated as repeat elements by RepeatMasker, while a small fraction of them map to lncRNAs or coding gene loci (Supplementary information, Figure S7C). Intriguingly, the predicted target sites exhibit more matches to non-repeat loci in mRNAs, although non-repeat-derived piRNAs only represent a small portion of the 1 295 predicted mRNA-targeting piRNAs (Supplementary information, Figure S7D). In contrast, many repeat-derived piRNAs seem to target a small number of the same sites in mRNAs (Supplementary information, Table S1).
Previous studies reported that piRNAs from adult mouse testes generally do not show signature of ping-pong cycle biogenesis26,27. Unexpectedly, we found that this group of mRNA-targeting piRNAs possesses both key signatures of the ping-pong mechanism, i.e., the typical bias of U at the 5′ end and A at position 10 of piRNAs (Figure 5D, top), which differ from the majority of MIWI-associated piRNAs in adult mouse testes26,27. Moreover, consistent with these features in mRNA-targeting piRNAs, we observed the corresponding bias for U at the mRNA cleavage site and for A 10 nt downstream of the U residue (Figure 5D, bottom). Finally, we found a few piRNAs corresponding to the predicted target sites in mRNAs. Together, these observations indicate that a 'ping-pong'-like mechanism may be responsible for generation of piRNAs from their target mRNAs.
Temporal regulation of piRNA target genes during mouse spermatogenesis
In Miwi−/ADH and Miwi−/− mice, germ cell development was uniformly blocked at the round spermatid stage17, suggesting that the MIWI slicer activity-mediated target repression may represent a critical mechanism in regulating spermatogenesis. In support of this notion, we found a significant increase in the mRNA levels of 169 predicted piRNA target genes in Miwi−/ADH round spermatids compared to Miwi+/− cells (Figure 2D and Supplementary information, Figure S2B). Also, mRNA levels of several piRNA target genes were significant decreased in round spermatids compared with those in spermatocytes (Supplementary information, Figure S5A). Moreover, global expression profiling analysis showed that the mRNA levels of 169 predicted piRNA target genes in elongating spermatids (GSE59291) were significantly lower than those in round spermatids (GSE32180; Supplementary information, Figure S8), suggesting a temporal regulation of piRNA target genes during mouse spermatogenesis.
Functional importance of temporal regulation of piRNA target genes for spermiogenesis in mice
To probe the functional importance of temporal regulation of piRNA target genes, we first analyzed annotated functions for the 169 predicted target genes using DAVID28. Interestingly, we found that these genes are enriched in categories of DNA damage and repair, vacuole, zinc binding, cell aging and ATP binding (Supplementary information, Table S2). Importantly, 14 out of these 169 predicted piRNA target genes have been shown to be essential for spermatogenesis in mouse models (Supplementary information, Table S3). These results suggest that silencing of genes by MIWI/piRNAs is functionally linked to male germ cell development.
To experimentally prove the biological requirement for proper regulation of piRNA targets during mouse spermatogenesis, we further characterized two specific piRNA target genes, Rad1 and Xrcc2, which are known to play critical roles in DNA recombination and repair29,30. We first validated the predicted piRNA:mRNA interactions using a reporter-based assay, confirming Rad1 and Xrcc2 as authentic piRNA targets (Figure 6A and Supplementary information, Figure S9A). We next demonstrated in wild-type mice that Rad1 and Xrcc2 protein levels were greatly reduced in round spermatids compared to those in spermatocytes (Figure 6B), indicating a temporal regulation of these two piRNA targets during mouse spermatogenesis. Conversely, the expression of Rad1 and Xrcc2 was upregulated in either Miwi−/− or Miwi−/ADH round spermatids (Figure 6C, 6D and Supplementary information, Figure S9B), suggesting that the slicer activity of MIWI is required for downregulation of these genes in mouse spermatids.
To determine the functional requirement of regulated expression of Rad1 and Xrcc2 for the formation of sperms in mice, we respectively cloned their cDNAs into a lentiviral vector upstream of the IRES-GFP (Supplementary information, Figure S10A), and used the spermatid-specific protamine 1 promoter (Prm) to drive their expression in spermatids through testis transduction. To confirm the expression of transduced Rad1 or Xrcc2, we isolated haploid spermatids from the transduced testes using the unit gravity sedimentation procedure as we described previously21 and by western blotting, we found that the Rad1 or Xrcc2 protein levels were significantly increased in spermatids isolated from the expression vector-transduced testes compared to control vector-transduced testes (Supplementary information, Figure S10B; n = 5 in each group), demonstrating successful lentiviral transduction as well as spermatid expression of Rad1 or Xrcc2 protein in transduced mice.
We next scored the effects of lentiviral transducted Rad or Xrcc2 on the formation of sperms in epididymides by PCR as we described previously21. Wereasoned that if the transducted GFP-containing vector does not affect normal sperm maturation, GFP DNA should be readily detectable in genomic DNA from epididymal spermatozoa by PCR. Consistent with our previous observation21, GFP DNA was readily detected in genomic DNA from epididymal spermatozoa in five out of five mice transduced with control vector (Figure 6E, lanes 4-8; n = 5). In sharp contrast, we could not detect any GFP DNA signal in sperm genomic DNA prepared from mice transduced with either Rad1 or Xrcc2 vector (Figure 6E, lanes 10-20; n = 5), indicating that spermatids expressing exogenous piRNA-resistant Rad1 or Xrcc2 failed to progress to mature sperm. Collectively, these results suggest that the temporal regulation of piRNA targets in spermatids is essential for subsequent sperm formation.
The role of piRNAs in transposon silencing and epigenetic regulation is well established. Recent studies show that some piRNAs in Drosophila appear to have the capacity to regulate the stability of several mRNAs31. However, in light of two recent studies suggesting that MIWI may bind and translationally repress spermatogenic mRNAs in a piRNA-independent manner in mouse testes27,32, it has been unclear whether mammalian piRNAs may participate in post-transcriptional regulation of protein-coding genes and, if so, whether this represents another major mode of piRNA actions. Based on our computational analyses, genome-wide mapping of MIWI-RNA interactions, and reporter-based assays, we present a series of findings that demonstrate the function of a subset of piRNAs in the regulation of mRNA stability in mouse testes by a mechanism that requires the slicer activity of the MIWI protein.
First, we identified potential MIWI-cleaved mRNA fragments in a published 5′-RACE library17. We also deduced that potential piRNAs may be target specific sites in these mRNAs based on base pairing from nucleotides 2 to 21 in the piRNAs. Consistent with the action site of the MIWI slicer activity on its targets17, we found that the 5′ ends of the predicted targeting piRNAs frequently match position 10 from the 5′ ends of mRNA fragments. This correlation was not observed in libraries from testes of MIWI catalytic mutant mice, and the expression levels of the predicted piRNA target genes were significantly upregulated in the mutant testes. Importantly, our MIWI CLIP-seq data indicate that the MIWI protein is physically associated with the predicted piRNA target sites in mRNAs. Our reporter assays further demonstrated that piRNAs could induce downregulation of their targets, and both the slicer activity and piRNA-loading ability of MIWI are required for such piRNA-guided target repression. Taken together, these data strongly suggest that piRNA-guided mRNA degradation is a predominant mode of MIWI/HIWI functions in mammals. Considering that cleaved mRNA fragments are rapidly degraded by other cellular nucleases, we predict that the piRNA system may play widespread roles in mediating mRNA degradation in male germ cells.
Remarkably, we found that base pairing at the first base of piRNAs is important for piRNA-mediated mRNA cleavage in vivo, based on our computational analyses of mRNA cleavage sites in mouse testicular 5′-RACE cleavage fragment library17. Consistently, our observation of 1U and 10A signatures at the cleavage sites suggests a ping-pong amplification-like mechanism for piRNA action on mRNA, further supporting the importance of the first base pairing in this process. In contrast, based on the structure of AGO silencing complexes, the first base of guide RNAs interacts with the Mid domain and does not form duplex with target RNAs33, suggesting that the first base pairing of guide RNAs to targets is dispensable for AGO/siRNA-mediated gene silencing. Moreover, while previous biochemical studies showed that base pairing beyond position 16 in AGO silencing complexes appears to be dispensable33, base pairing at position 20 and beyond still significantly affects MIWI-mediated cleavage of target RNAs17. Future studies of the crystal structure of MIWI silencing complexes will provide critical information to understand the molecular mechanism underlying piRNA-mediated gene silencing.
We note that another mouse Piwi protein MILI has also been shown to possess slicer activity critical for post-transcriptional silencing of retrotransposon LINE1 in spermatocytes18,34. However, our results demonstrate that the expression levels of the predicted piRNA target genes are significantly upregulated in Miwi−/ADH testes (Figure 2D), in which Mili is normally expressed. This finding implies that MILI may not be involved in the regulation of this group of piRNA targets identified in our study. Nevertheless, it remains to be determined whether MILI plays a role in post-transcriptional regulation of other coding genes in mouse testes.
In summary, our present study combined computational and experimental approaches to deduce rules for piRNA:mRNA interactions through analyzing sequence complementarity, location of target sites, as well as piRNA reads that match their target sites. Even though the rules that we deduced are subject to further refinement, our data suggest that the productive piRNA:mRNA interactions require (1) a minimal ∼16-nt continuous complementarity between piRNAs and mRNAs, (2) strict base pairing at the 5′ end of piRNAs, (3) < 3 mismatches in subsequent 20 nt sequences, (4) conserved 3′-UTRs as preferred piRNA target sites, and (5) U and A residues enriched at the 1st and 10th positions in piRNAs that target specific mRNAs. Given that millions of piRNAs are expressed in mouse testes and their roles in silencing transposons have been well-documented, it will be of significance in future studies to determine which portion of the piRNA machinery is devoted to protecting the genome against transposable elements and which portion is involved in the regulation of protein-coding genes, as well as these two processes is coordinated in different stages of male germ cell development in mammals.
Materials and Methods
Plasmids, RNA oligonucleotides, qPCR primers and antibodies
For reporter pmirGLO-Psma8 3′-UTR, the 860-bp mouse Psma8 3′-UTR was cloned into pmirGLO vector (Promega) downstream of the Firefly luciferase gene. Eight nucleotides in Psma8 3′-UTR complementary to the 5′ portion of piR-671 were deleted using KOD-Plus-mutagenesis kit (Toyobo) to generate reporter Psma8 3′-UTR Mut. The wild-type and mutant 3′-UTR reporters for other target candidates, including Tox4, BC026590, Xrcc2, and Rad1, were similarly constructed (Figures 4B, 4C, 6A and Supplementary information, Figures S5B-S5D and S9A). p3×Flag-MIWI, p3×Flag-HIWI, and p3×Flag-HIWI mutants were constructed as recently described20. pLV-Prm-RAD1-IRES-GFP and pLV-Prm-XRCC2-IRES-GFP were derived from vector Lenti-EF1α-Oct4-IRES-EGFP (SIDANSAI) as previously described21, in which the Oct4 promoter was replaced by the 0.6 kb of the protamine1 promoter (Prm) sequences (Supplementary information, Figure S10A). All constructs were confirmed by Sanger sequencing. All RNA oligonucleotides, including piRNAs and siRNAs, were synthesized at Ribobio (Guangzhou, China), and the sequences are provided in Supplementary information, Table S4. Primer sequences for RT-qPCR are shown in Supplementary information, Table S5.
Mouse monoclonal anti-β-actin (A3854) antibody was purchased from Sigma, and anti-XRCC2 (sc-5895) was from Santa Cruz. Rabbit anti-Rad1 polyclonal antibodies (A1047) was from ABclonal Technology and anti-GFP (MBL-598) was from MBL.
The global 5′-RACE cleavage fragment data, gene expression profile, and MIWI-immunoprecipitated piRNA data in the control and catalytic mutant mouse testes were obtained from the SuperSeries in GEO: GSE3218317. We used the processed sequencing data generated from adult mice. Mouse genome data (mm9) and RefSeq mRNA data were downloaded from UCSC35,36.
Global 5′-RACE sequences were mapped to the mouse genome and RefSeq mRNAs using bowtie37 with no mismatches. When the 5′-RACE tags were mapped to mRNA isoforms of the same gene, only one mapping result was kept for further analyses. For piRNA target mapping, when applying the rule that 1-3 mismatches were allowed in piRNA positions 2-21, we extracted sequences from positions 2 to 21 of piRNA and aligned them to mRNAs using bowtie, allowing up to 3 mismatches. Otherwise, we used blastn with word size –W 8 and output format –m 1 and then summarized the details of the matches, mismatches and gaps using custom Perl scripts. G-U wobble base pairs were regarded as mismatches in our analyses.
Distribution of the number of potential piRNAs binding in the vicinity of mRNA fragments
For each cleaved mRNA fragment, we counted the number of unique piRNAs that could potentially bind to the mRNA at different distances from the 5′ end of the mRNA fragment to the 5 end of the piRNA.
We summed up the number of the piRNAs that could potentially bind at each position relative to all mRNA fragments. The piRNA data were obtained from controls (GSM822760 Miwi+/+) or MIWI catalytic mutants (GSM822762-4 Miwi−/ADH, 3 datasets). The mRNA fragment data were also extracted from controls (GSM822765 Miwi+/−) or MIWI catalytic mutants (GSM822766 Miwi−/ADH)17.
Analysis of public microarray data
Raw data (CEL file format) for 8 samples of round spermatids from adult mouse testes in GSE3218017 were loaded into R38 using the affy package, normalized, and converted to log2 gene expression by rma using the “mogene10stv1mmentrezgcdf” cdf package downloaded from customCDF (version 15) on the brainarray website39. The raw data for 6 samples of elongating spermatids (GSE59291) (Microarray performed in Shanghai Biotechnology Corporation)20 were processed in the same way using the “mouse4302mmentrezgcdf” cdf package. For the comparison of gene expression levels between these two platforms, expression levels were further normalized by the “normalizeBetweenArrays” tool in limma package.
Probe level expression of the GSE32180 microarray data was calculated using rma in R, and all MIWI catalytic mutant (Miwi−/ADH) and control (Miwi+/−) samples were extracted and converted to gct format for GSEA. MoGene_1_0_st.chip was used as platform annotation. Probe level expressions were collapsed to the gene level according to the gene annotation. A new set of potential piRNA target genes was created. Gene symbols were modified manually to fit the platform annotation. For permutation type, we chose gene_set according to the GSEA suggestion. Other parameters were set as default.
MIWI CLIP-seq and data processing
MIWI CLIP-seq was performed as described40. In brief, isolated round spermatids from adult C57BL/6J mice were UV-irradiated (254 nm) at 400 mj in a 15-cm plate before immunoprecipitation using a highly specific anti-MIWI antibody. Immunoprecipitated RNA-protein complexes were digested with micrococcal nuclease, labeled with [g-32P]-ATP (PerkinElmer) by T4 PNK (Fermentas), and isolated by SDS-PAGE, from which [32P]-labeled RNA-protein bands were cut for extracting RNAs for linker ligation, PCR amplification, and deep sequencing.
For each read, the first 4 random index sequences were removed and appended to the read name. The resulting read was 36 nt in length. The 3′-adaptor sequence (IndexTermCTCGTATGCCGTCTTCTGCTTG) was trimmed and short reads (< 16 nt) were filtered out. Reads were then mapped to the mouse genome (mm9) using bowtie37 with parameters: “-l25 -n2 -k101 -m100 -e200 --best --strata --sam --phred33-quals”. Mapped reads with ≤ 1 mismatch were chosen for downstream analysis. Reads with lengths of 25-33 nt were classified as piRNAs, and longer reads are considered as MIWI targets.
Cell culture and transfection
GC-2spd(ts) cells were obtained from the American Type Culture Collection (ATCC) and cultured with the medium and serum as ATCC recommended. Transfection was performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions. For transfection of the RNA oligonucleotides, 100 nM of piRNA or scrambled siRNA oligonucleotides were used. For plasmid transfection, 4 μg DNA was used in a 6-well plate.
Luciferase reporter assay
Luciferase reporter assays were carried out as described previously20. In brief, each 3′-UTR luciferase reporter construct was cotransfected into GC-2spd(ts) cells in 24-well plates with RNA oligonucleotides or vectors as indicated in Figure 4 and Supplementary information, Figure S5. piRNA negative controls were designed as the mutated forms of their cognate piRNAs (designated as piR-xxx(Mut)) by altering the first 8-nt sequences at the 5′ end to disrupt base pairing between piRNAs and target mRNAs. The sequences of piRNAs and their corresponding mutants are provided in Supplementary information, Figures S5B and S9A. All experiments were triplicated and P values were calculated by Student's t-test.
Northern blot was carried out according to standard protocols. In brief, 15 μg of total RNAs were loaded per lane on 1% formaldehyde agarose gel. After electrophoresis, samples were transferred to nylon membrane (Hybond N+, GE, USA) and probed with a ∼1-kb ORF fragment of target genes that were labeled with [α-32P]dCTP.
Ectopic expression of Rad1 or Xrcc2 in mouse spermatids
Lentivirus packaging and testis transduction were performed as described previously21. In brief, under a microscope, testes of pentobarbital sodium-anesthetized mice were pulled out and ∼10 μl of high-titer lentivirus were injected into each seminiferous tubule through efferent duct by a sharp glass capillary with a tip diameter of 50 μm. The testes were then returned to the abdominal cavity, and the abdominal wall and skin were closed with sutures. The testes were harvested 4-5 weeks after transduction of ectopic Rad1 or Xrcc2 expression vectors.
Isolation of mouse spermatogenic cells and sperms
Mouse total germ cells were extracted from the seminiferous tubules of adult male mice, and spermatocytes and haploid spermatids were isolated using a unit gravity sedimentation procedure as described previously21. Epididymal sperm isolation was carried out following standard procedures. In brief, epididymides were removed immediately after male mice were sacrificed, and placed into sterile Petri dishes containing ice-cold PBS. By gently shearing the tissues and rupturing the tubules, sperms were isolated from epididymides and diffused into the medium. Finally, spermatozoa were obtained by gently filtering on ice through fine nylon mesh to remove tissue debris.
All potential piRNA target sites in the mouse genome were located. Duplicate sites located too close to one another (within 10 nt) were removed manually. The non-redundant sites obtained were uploaded to cistrome41, and conservation analysis was performed using Conservation Plot in Integrative Analysis. Since Conservation Plot does not consider strand information, sites belonging to the plus and minus strands of individual chromosomes were separated before conservation analysis, and then the minus strand results were reversed and averaged with the plus strand results.
Availability of supporting data
The names of piRNA sequences used in the reporter assays, such as piR-mmu-77755, are available at piRBase24 (http://www.regulatoryrna.org/database/piRNA/). The additional files and information are available at: http://www.regulatoryrna.org/pub/miwi_cleave/index.html.
Thomson T, Lin H . The biogenesis and function of PIWI proteins and piRNAs: progress and prospect. Annu Rev Cell Dev Biol 2009; 25:355–376.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA . A germline-specific class of small RNAs binds mammalian Piwi proteins. Nature 2006; 442:199–202.
Aravin A, Gaidatzis D, Pfeffer S, et al. A novel class of small RNAs bind to MILI protein in mouse testes. Nature 2006; 442:203–207.
Lau NC, Seto AG, Kim J, et al. Characterization of the piRNA complex from rat testes. Science 2006; 313:363–367.
Grivna ST, Beyret E, Wang Z, Lin H . A novel class of small RNAs in mouse spermatogenic cells. Genes Dev 2006; 20:1709–1714.
Siomi MC, Sato K, Pezic D, Aravin AA . PIWI-interacting small RNAs: the vanguard of genome defence. Nat Rev Mol Cell Biol 2011; 12:246–258.
Brennecke J, Aravin AA, Stark A, et al. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell 2007; 128:1089–1103.
Gunawardane LS, Saito K, Nishida KM, et al. A slicer-mediated mechanism for repeat-associated siRNA 5′ end formation in Drosophila. Science 2007; 315:1587–1590.
Aravin AA, Naumova NM, Tulin AV, Vagin VV, Rozovsky YM, Gvozdev VA . Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline. Curr Biol 2001; 11:1017–1027.
Nishida KM, Saito K, Mori T, et al. Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad. RNA 2007; 13:1911–1922.
Saito K, Inagaki S, Mituyama T, et al. A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. Nature 2009; 461:1296–1299.
Rouget C, Papin C, Boureux A, et al. Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. Nature 2010; 467:1128–1132.
Juliano C, Wang J, Lin H . Uniting germline and stem cells: the function of Piwi proteins and the piRNA pathway in diverse organisms. Annu Rev Genet 2011; 45:447–469.
Aravin AA, Sachidanandam R, Bourc'his D, et al. A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice. Mol Cell 2008; 31:785–799.
Kuramochi-Miyagawa S, Watanabe T, Gotoh K, et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev 2008; 22:908–917.
Watanabe T, Tomizawa S, Mitsuya K, et al. Role for piRNAs and noncoding RNA in de novo DNA methylation of the imprinted mouse Rasgrf1 locus. Science 2011; 332:848–852.
Reuter M, Berninger P, Chuma S, et al. Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing. Nature 2011; 480:264–267.
De Fazio S, Bartonicek N, Di Giacomo M, et al. The endonuclease activity of Mili fuels piRNA amplification that silences LINE1 elements. Nature 2011; 480:259–263.
Subramanian A, Tamayo P, Mootha VK, et al. Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles. Proc Natl Acad Sci USA 2005; 102:15545–15550.
Gou LT, Dai P, Yang JH, et al. Pachytene piRNAs instruct massive mRNA elimination during late spermiogenesis. Cell Res 2014; 24:680–700.
Zhao S, Gou LT, Zhang M, et al. piRNA-Triggered MIWI ubiquitination and removal by APC/C in late spermatogenesis. Dev Cell 2013; 24:13–25.
Deng W, Lin H . Miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell 2002; 2:819–830.
Hofmann MC, Abramian D, Millan JL . A haploid and a diploid cell coexist in an in vitro immortalized spermatogenic cell line. Dev Genet 1995; 16:119–127.
Zhang P, Si X, Skogerbø G, et al. piRBase: a web resource assisting piRNA functional study. Database (Oxford) 2014 Nov 25. doi: 10.1093/database/bau110
Siepel A, Bejerano G, Pedersen JS, et al. Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes. Genome Res 2005; 15:1034–1050.
Beyret E, Liu N, Lin H . piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism. Cell Res 2012; 22:1429–1439.
Vourekas A, Zheng Q, Alexiou P, et al. Mili and Miwi target RNA repertoire reveals piRNA biogenesis and function of Miwi in spermiogenesis. Nat Struct Mol Biol 2012; 19:773–781.
Huang DW, Sherman BT, Lempicki RA . Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc 2009; 4:44–57.
Deans B, Griffin CS, O'Regan P, Jasin M, Thacker J . Homologous recombination deficiency leads to profound genetic instability in cells derived from Xrcc2-knockout mice. Cancer Res 2003; 63:8181–8187.
Lyndaker AM, Lim PX, Mleczko JM, et al. Conditional inactivation of the DNA damage response gene Hus1 in mouse testis reveals separable roles for components of the RAD9-RAD1-HUS1 complex in meiotic chromosome maintenance. PLoS Genet 2013; 9:e1003320.
Peng JC, Lin H . Beyond transposons: the epigenetic and somatic functions of the Piwi-piRNA mechanism. Curr Opin Cell Biol 2013; 25:190–194.
Nishibu T, Hayashida Y, Tani S, et al. Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. Biosci Trends 2012; 6:248–261.
Wang Y, Juranek S, Li H, et al. Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes. Nature 2009; 461:754–761.
Di Giacomo M, Comazzetto S, Saini H, et al. Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis. Mol Cell 2013; 50:601–608.
Waterston RH, Lindblad-Toh K, Birney E, et al. Initial sequencing and comparative analysis of the mouse genome. Nature 2002; 420:520–562.
Karolchik D, Hinrichs AS, Furey TS, et al. The UCSC Table Browser data retrieval tool. Nucleic Acids Res 2004; 32:D493–D496.
Langmead B . Aligning short sequencing reads with Bowtie. Curr Protoc Bioinformatics 2010; Chapter 11:Unit 11.7.
R_Development_Core_Team. R: A language and environment for statistical computing 2009.
Dai M, Wang P, Boyd AD, et al. Evolving gene/transcript definitions significantly alter the interpretation of GeneChip data. Nucleic Acids Res 2005; 33:e175.
Xue Y, Ouyang K, Huang J, et al. Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits. Cell 2013; 152:82–96.
Liu T, Ortiz JA, Taing L, et al. Cistrome: an integrative platform for transcriptional regulation studies. Genome Biol 2011; 12:R83.
Crooks GE, Hon G, Chandonia JM, Brenner SE . WebLogo: a sequence logo generator. Genome Res 2004; 14:1188–1190.
We are grateful to Xiaole Shirley Liu and Jianhua Yang for thoughtful discussions and valuable comments on the manuscript. This work was supported by grants from the Ministry of Science and Technology of China (2011CB504605 and 2014AA021103), the National Natural Science Foundation of China (31090253, 31210103912, 31325008 and 91419307), the Chinese Academy of Sciences (CAS; KJZD-EW-L01-2), the Key Laboratory of the Zoological Systematics and Evolution of the Chinese Academy of Sciences (O529YX5105), and the National Science Fund for Fostering Talents in Basic Research (Special subjects in animal taxonomy, NSFC-J1210002). This work was also supported by NIH grants (GM052872 and HG004659). The computing resource was supported by HPC Platform, Scientific Information Center, Institute of Zoology, CAS.
( Supplementary information is linked to the online version of the paper on the Cell Research website.)
(A) mRNA fragment reads and targeting piRNAs in Mdc1 3′ UTR. (PDF 1408 kb)
(A) The distribution of the mRNA fragments that have targeting piRNAs at each relative distance. (PDF 130 kb)
(A) GSEA enrichment plot using the gene expression profile GSE32180 data. (PDF 196 kb)
(A) Autoradiogram of 32P-labelled RNA crosslinked to MIWI purified by immunoprecipitation from enriched mouse round spermatids. (PDF 1945 kb)
(A) RT-qPCR analyses of the mRNA levels of Atr, BC026590, Gfpt1, Mdc1, Ppp1cb, Psma8, Tox4, Adam3, Csnk2b, and Fabp9 in enriched Miwi+/− spermatocytes and round spermatids (top), or in enriched Miwi+/− spermatocytes and round spermatids (bottom). (PDF 503 kb)
Exploration of the rules for piRNA binding to mRNA targets. (PDF 1491 kb)
(A) The size distribution of the mRNA-targeting piRNAs. (PDF 475 kb)
Accumulative distribution of expression levels of piRNA target genes in round spermatids (RS, GSE32180) and elongating spermatids (ES, GSE59291). (PDF 97 kb)
(A) Predicted piRNA regulatory elements at the 3′UTRs of Xrcc2, and Rad1, and the sequences of synthetic piRNAs, negative control piRNA (Mut), and wild-type 3′UTR and mutated 3′UTR Mut luciferase reporters. (PDF 1547 kb)
(A) Construction of Xrcc2-IRES-EGFP or Rad1-IRES-EGFP lentiviral vector driven by protamine 1 promoter (Prm). (PDF 310 kb)
Predicted piRNA target sites. (PDF 68 kb)
Functional annotation of predicted piRNA target genes. (XLSX 91 kb)
Predicted piRNA target genes that have been shown to be essential for spermatogenesis in mouse. (PDF 92 kb)
Sequences of chemically synthesized oligonucleotides (PDF 54 kb)
Primer sequences. (PDF 61 kb)
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Zhang, P., Kang, JY., Gou, LT. et al. MIWI and piRNA-mediated cleavage of messenger RNAs in mouse testes. Cell Res 25, 193–207 (2015). https://doi.org/10.1038/cr.2015.4
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