Cushing's disease, also known as adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas (PAs) that cause excess cortisol production, accounts for up to 85% of corticotrophin-dependent Cushing's syndrome cases. However, the genetic alterations in this disease are unclear. Here, we performed whole-exome sequencing of DNA derived from 12 ACTH-secreting PAs and matched blood samples, which revealed three types of somatic mutations in a candidate gene, USP8 (encoding ubiquitin-specific protease 8), exclusively in exon 14 in 8 of 12 ACTH-secreting PAs. We further evaluated somatic USP8 mutations in additional 258 PAs by Sanger sequencing. Targeted sequencing further identified a total of 17 types of USP8 variants in 67 of 108 ACTH-secreting PAs (62.04%). However, none of these mutations was detected in other types of PAs (n = 150). These mutations aggregate within the 14-3-3 binding motif of USP8 and disrupt the interaction between USP8 and 14-3-3 protein, resulting in an elevated capacity to protect EGFR from lysosomal degradation. Accordingly, PAs with mutated USP8 display a higher incidence of EGFR expression, elevated EGFR protein abundance and mRNA expression levels of POMC, which encodes the precursor of ACTH. PAs with mutated USP8 are significantly smaller in size and have higher ACTH production than wild-type PAs. In surgically resected primary USP8-mutated tumor cells, USP8 knockdown or blocking EGFR effectively attenuates ACTH secretion. Taken together, somatic gain-of-function USP8 mutations are common and contribute to ACTH overproduction in Cushing's disease. Inhibition of USP8 or EGFR is promising for treating USP8-mutated corticotrophin adenoma. Our study highlights the potentially functional mutated gene in Cushing's disease and provides insights into the therapeutics of this disease.
Adrenocorticotropic hormone (ACTH)-secreting pituitary adenomas (PAs), referred to as Cushing's disease, arise from pituitary corticotroph cells and secrete large amounts of ACTH, promoting the adrenal glands to produce elevated levels of cortisol and then induce endogenous hypercortisolism1,2. Cushing's disease accounts for up to 85% of corticotrophin-dependent Cushing's syndrome cases3. Cushing's disease is a severe and potentially fatal disease. Patients may have many clinical features such as central obesity, hypertension, cardiovascular disease and metabolic syndrome2,4, which are variably present in any given case. Both the diagnosis of hypercortisolism and the preoperative location of PAs in Cushing's disease are complicated and sometimes difficult1,4. Currently, the first-line treatment of Cushing's disease is pituitary adenomectomy, generally performed via a transsphenoidal approach. However, only some patients achieve initial remission after surgery and PAs tend to recur after periods of remission5, emphasizing the need to develop novel therapeutic approaches for Cushing's disease.
ACTH hypersecretion is a hallmark of Cushing's disease and reported to be caused by several molecular defects. For example, downregulation of Brg1 and histone deacetylase 2 (HADC2) is found in Cushing's disease, which promotes proopiomelanocortin (POMC) transcription6. Reduced expression of the cell cycle inhibitor p27 (Kip1) is detected in human Cushing's adenoma7, and mice with disrupted p27(Kip1) develop ACTH-secreting PAs8. High expression of EGFR is frequently observed in ACTH-secreting PAs and blocking EGFR signaling effectively inhibits ACTH secretion9,10. However, the genetic basis for these molecular alterations remains elusive.
Several genetic alterations have been described in PAs. For example, somatic activating mutations of guanine nucleotide-binding protein α subunit (GNAS) are found in 30%–40% of sporadic somatotroph adenomas and account for McCune-Albright syndrome accompanied by PAs11,12. Germline AIP mutations are found in sporadic PA patients without a family history of this disease13,14. However, ACTH-secreting PAs are rarely reported to associate with adenoma-predisposing syndromes as well as mutations of GNAS or AIP15. Although case reports of mutations in p5316, DAX-117, DICER118 or the glucocorticoid receptor gene NR3C119 have been described in ACTH-secreting PAs or pituitary blastomas, the prevalent genetic alterations in these adenomas are still unknown.
In this study, we identified a total of 17 types of USP8 (ubiquitin-specific protease 8) variants, which occurred in more than 60% of ACTH-secreting corticotrophin adenomas. These mutations could induce ACTH overproduction via deregulation of EGFR signaling and may lead to different clinical phenotypes. We further showed that USP8 knockdown or gefitinib (a clinically available EGFR inhibitor) treatment significantly reduced ACTH secretion in primary USP8-mutated corticotrophin adenoma cells, but not in wild-type cells.
Somatic USP8 mutations in Cushing's disease
To search for the genetic alterations in ACTH-secreting PAs, whole-exome sequencing was performed in DNA from 12 tumors and matched blood samples (Supplementary information, Table S1). Mean tumor purity was estimated as 90%. The average sequencing depth was 74× (42× to 162×) for the tumors and 113× (47× to 175×) for blood (Supplementary information, Figure S1A). Furthermore, 94% (91%-95%) of target regions were covered by at least 10× sequencing depth (Supplementary information, Figure S1B). This study revealed a low number of somatic mutations (median, 5; range, 1-9) per case and a total of 45 non-synonymous and 12 synonymous somatic mutations (Figure 1A and Supplementary information, Table S2), which were further validated by Sanger sequencing.
Except for nonsense mutation of NR3C1 in one patient, no mutations were detected in any previously reported genes associated with adrenal Cushing's syndrome or PAs, including GNAS or PRKACA (Figure 1A). Strikingly, three recurrent somatic mutations (c.CTC2151-2153del/p.S718del; c.C2159G/p.P720R; c.T2152C/p.S718P), exclusively in exon 14 of the USP8 gene, were found in 8 out of 12 tumors (Figure 1A). Sanger sequencing revealed that there was no allelic imbalances of USP8 mutations in tumor tissues (Supplementary information, Figure S2), indicating a heterozygous status of these mutations, which is consistent with the observation by exome sequencing that their variant allele frequencies were comparable to those of other somatic mutations in tumor cells (Supplementary information, Figure S3). Except for USP8 mutations, no other mutations occurred in more than one tumor tissue (Supplementary information, Table S2). Finally, no copy number variation (CNV) of the USP8 gene was detected in 12 ACTH-secreting PAs based on whole-exome sequencing data.
To study the prevalence of USP8 mutations in various PAs, we screened an additional 258 PAs including 108 ACTH-secreting, 50 growth hormone (GH)-secreting, 50 prolactin (PRL)-secreting and 50 non-functioning (NF) PAs (Supplementary information, Table S1). In addition to the three types of USP8 mutations detected above, targeted sequencing identified other 14 types of somatic USP8 mutations distributed in 67 out of 108 ACTH-secreting PAs (62.04%, Figure 1B and Supplementary information, Figure S4). Notably, the S718del, P720R and S718P mutations account for the vast majority of the USP8-mutated cases (77.61%; Figure 1B). All these mutations were not detected in matched blood samples. All the 17 types of detected USP8 mutations were not found in dbSNP v138 and 1000 Genomes Project. Except for c.C2159G/p.P720R and C2159A/p.P720Q mutations, other mutations were not found in the COSMIC v68 database, indicating that these mutations are novel somatic mutations. Strikingly, none of the other types of PAs had similar genetic alterations in exon 14 of USP8 (Supplementary information, Table S1). Taken together, these data indicate that USP8 mutations are common and unique genetic alterations in ACTH-secreting PAs.
Functional characterization of USP8 mutants
USP8 is a deubiquitinating enzyme that protects growth factor receptors including epidermal growth factor receptor (EGFR) from degradation20,21. EGFR activation promotes POMC transcription in primarily cultured human ACTH-secreting tumor cells10. USP8 contains a putative 14-3-3 binding motif RSXSXP that is encoded by part of exon 14 of USP8 in humans (Figure 1B) and is identically conserved in various species (Supplementary information, Figure S5). The phosphorylation of USP8 on the fourth serine (S718) of its 14-3-3 binding motif results in its binding with 14-3-3 proteins and catalytic inactivation22,23. A structural study demonstrated that within the 14-3-3 binding motif, the amino acid preferences in each position are very similar, and the phosphorylation of serine at the fourth position is essential for its binding ability24. Furthermore, this serine (S718) was most frequently disrupted via deletion or missense mutation in all USP8-mutated cases (37/75). Other affected amino acids were either in the 14-3-3 binding motif or its adjacent regions (Figure 1B). Thus, we examined whether three prevalent tumor-derived USP8 mutations abolish or reduce USP8 binding ability with 14-3-3ε and increase the capability of blocking EGFR degradation.
As expected, WT USP8 was shown to bind 14-3-3ε in a GST pull-down assay. Interestingly, the three tested USP8 mutants were not detected in the 14-3-3ε bound complexes, although they were expressed at levels similar to WT (Figure 2A). Moreover, EGFR ubiquitination was obviously reduced in cells transduced with mutant USP8 (Figure 2B). EGF treatment induced a faster degradation of EGFR protein in HeLa cells expressing WT USP8 compared to mutants, although EGFR protein levels were comparable in these cells under serum-starved conditions (Figure 2C). Finally, EGF treatment induced a stronger phosphorylation of Erk1/2 in HeLa cells expressing mutant USP8 (Figure 2D).
We next assessed the consequences of USP8 mutation in tumor tissue. Immunochemical analysis demonstrated that the USP8 protein was ubiquitously expressed in normal pituitary tissue and in USP8 WT or mutated ACTH-secreting PAs at comparable levels (Figure 3A). However, only part of PAs were positive for EGFR staining, and EGFR-positive cases were more frequently detected in USP8-mutated PAs (P = 0.01, Pearson's χ2 test), where they account for ∼80% of the cases (28/35), compared to 50% in WT cases (15/30). Notably, EGFR staining intensity in EGFR-positive tumors with WT USP8 was generally lower (Figure 3A). Furthermore, immunoblot analysis demonstrated notably increased EGFR protein levels in half USP8-mutated PAs and Erk1/2 phosphorylation in most USP8-mutated PAs examined (Figure 3B and Supplementary information, Figure S6). RT-qPCR analysis revealed a higher mRNA abundance of POMC (P = 0.009, Mann-Whitney U-test), but not USP8 and EGFR, in ACTH-secreting PAs with USP8 mutations (Figure 3C). To assess the important role of USP8 mutation in ACTH production, we established surgically resected primary human ACTH-secreting tumor cells. USP8 knockdown resulted in a significant reduction of ACTH secretion in culture media from USP8-mutated tumors cells (P < 0.05, Mann-Whitney U-test), but not WT tumor cells (Figure 3D). Treatment of gefitinib, a clinically available EGFR inhibitor, also effectively attenuated ACTH secretion in USP8-mutated tumor cells (Figure 3E). Moreover, USP8 knockdown reduced EGFR protein level in USP8-mutated tumor cells (Supplementary information, Figure S7). Taken together, these results indicate that USP8 mutations contribute to the pathogenesis of ACTH-secreting PAs.
Clinical phenotypes and USP8 mutational status
Few genetic alterations have been linked to the clinical phenotype of Cushing's disease. We explored whether patients with Cushing's disease carrying USP8 mutations display distinct clinical features. USP8 mutations were identified in both female and male patients, but their frequency was significantly higher in female patients (67.7%) compared to male patients (38.1%) (P = 0.01, Pearson's χ2 test; Figure 4A). Contrast-enhanced T1-weighted magnetic resonance imaging (MRI) demonstrated that 16 of 39 USP8-WT PAs (41.0%) were very large and extended into the parasellar space, including the cavernous sinus and the sphenoidal sinus. In contrast, the vast majority of USP8-mutated PAs was small in size (maximal diameter) and diffusely distributed within the sella, and only 8 of 63 cases (12.7%) displayed parasellar invasion (P = 0.001, Pearson's χ2 test; Figure 4B and 4C)25,26. This morphological difference is further supported by the fact that USP8-mutated PAs were generally smaller in size compared to USP8-WT PAs (P < 0.001, Mann-Whitney U-test; Figure 4D). However, patients with mutated USP8 showed comparable levels of plasma ACTH, midnight serum cortisol and 24-h urinary free cortisol (UFC; Table 1), indicating that USP8-mutated PAs have higher ACTH production.
The rate of immediate biochemical remission after initial transsphenoidal surgery was indistinguishable between USP8 WT and mutated patients. One month to nine years follow-up of patients receiving initial remission further demonstrated that the recurrence rate and the average recurrence period were unrelated to the USP8 mutational status (Table 1).
ACTH-secreting adenomas are monoclonal in nature, although sporadic in origin, and genetic alterations have been implicated in their tumorigenesis27,28. However, genetic lesions have seldom been reported in these tumors. Our current study revealed that 62.04% of ACTH-secreting PAs harbor somatic mutations in exon 14 of the USP8 gene. The number of somatic mutations per case was low, and except USP8 mutations, no other recurrent mutations were found in 12 ACTH-secreting PAs examined. Moreover, the CNV of the USP8 gene was not detected based on exome sequencing data from 12 ACTH-secreting PAs. Collectively, our study identified somatic USP8 mutations as the dominant genetic alterations in ACTH-secreting PAs, suggesting that Cushing's disease may result from genetic alterations. It is expected that other rare recurrent gene mutations may be found in Cushing's disease if we increase sample size for exome sequencing and/or examine mutational status of each of other 56 mutated genes (listed in Supplementary information, Table S2) in 108 PAs using Sanger sequencing. Importantly, none of other types of PAs harbor mutations in exon 14 of USP8. Previous studies reported that GNAS or AIP mutation-associated PAs usually secrete GH or PRL15. Genetic alteration of either the GNAS or AIP genes was rarely detected in ACTH-secreting PAs. Thus, our finding provides evidence that the genetic origin of PAs is heterogeneous and that ACTH-secreting PAs are pathogenically distinct. Of note, a nonsense mutation of NR3C1, resulting in a truncated glucocorticoid receptor, was detected in one WT-USP8 patient. Inactivation of the glucocorticoid receptor signal is involved in the pathogenesis of ACTH-secreting PAs, especially for ACTH overproduction29,30. Therefore, the NR3C1 mutation could be an important genetic lesion in some PAs, especially for WT-USP8 cases.
Strikingly, all three prevalent tumor-derived USP8 mutants fail to bind 14-3-3 proteins and display an elevated deubiquitinating activity toward EGFR, indicating that these represent gain-of-function mutations. Several lines of evidence support that USP8 mutation contributes to ACTH hyperproduction: (1) USP8-mutated PAs expressed higher level of POMC mRNA, (2) patients with USP8-mutated PAs had similar levels of plasma ACTH, although PAs were markedly smaller in size, and (3) USP8 knockdown attenuated ACTH secretion in primary USP8-mutated tumor cells. Therefore, we conclude that gain-of-function USP8 mutation plays an important role in ACTH overproduction.
USP8 deubiquinates numerous proteins, and some of them are implicated in ACTH production. As a well-studied USP8 target, EGFR appears to play a central role in ACTH hyperproduction in Cushing's disease carrying USP8 mutation. We found that USP8-mutated PAs have a higher incidence of EGFR expression, increased EGFR protein abundance and activation of downstream Erk1/2, indicating that USP8 mutations enhance EGFR signaling in tumors. Importantly, USP8 inactivation attenuated ACTH secretion and EGFR expression in primary tumor cells. Activation of EGFR promotes POMC transcription via MAPK signaling in Cushing's disease10. Taken together, we conclude that USP8 mutation sustains EGFR-MAPK signaling to promote ACTH production in Cushing's disease (Figure 5). Interestingly, low p27(Kip1) expression is more frequently observed in corticotropinoma expressing high level of EGFR9. Considering that activation of EGFR-MAPK signaling induces p27(Kip1) degradation, and p27(Kip1)-deficient mice develop corticotropinoma7,31, we speculate that through activating EGFR signaling USP8 mutation accelerates p27(Kip1) degradation, representing an important molecular mechanism underlying ACTH hyperproduction (Figure 5). In addition, USP8 regulates the expression of other receptor tyrosine kinases (RTKs) such as ErbB3 and C-Met32,33, which share very similar signaling cascades with EGFR. Indeed, we observed the high expression level of ErbB3 and C-Met in a substantial number of USP8-mutated PAs via immunochemistry (Supplementary information, Figure S8). Smoothened, a key component of Hedgehog pathway, is deubiquitinated by USP834 and activation of Hedgehog pathway induces ACTH secretion35. It is expected that USP8 mutations deregulate these molecules to drive ACTH production and secretion. Further studies are required to fully understand the role of USP8 mutations in the pathogenesis of ACTH-secreting PAs and the underlying molecular mechanisms.
Our findings have important clinical implications. Most USP8-mutated PAs are small in size (< 0.5 cm) and diffusely distributed within the sella, making them difficult to be detected by MRI scanning and even by those experienced neurosurgeons using intraoperative neuronavigation. In contrast, almost half of the USP8-WT PAs were very large and featured by parasellar invasion, increasing the difficulty and risk of surgery. In each situation, it is almost impossible to completely remove the entire tumor, which could explain the similarly low remission and high recurrence rate after initial surgery in both USP8 WT and mutated ATCH-secreting PAs. In addition, considering that USP8-mutated PAs usually display much smaller size, we speculate that patients with small tumor even difficultly detected by MRI scanning, most likely carry USP8 mutations. Therefore, inhibiting USP8 activity might be the first-line treatment of choice for patients with such PAs. As surgery alone seems to be unlikely to cure most cases of Cushing's disease, new therapeutic approaches are needed to specifically target tumor and suppress ACTH production1,5. Our findings strongly indicate that inhibiting USP8 catalytic activity might be a promising therapeutic strategy for Cushing's disease cases with USP8 mutations, especially for patients with either residual or recurrent adenomas. Moreover, anti-EGFR therapy, a potential therapeutic strategy for treatment of Cushing's disease10, seems to be more effective for patients carrying USP8 mutation, given that these patients more likely harbor activated EGFR-MAPK signaling.
While this manuscript was in preparation, Reincke et al.36 reported similar findings that dominant mutations in USP8 lead to Cushing's disease through activation of EGFR signaling. Of note, there are some significant differences between our study and study by Reincke et al. To our knowledge, our study which includes 120 corticotrophin adenomas is the largest genetic investigation for Cushing's disease so far. The study by Reincke et al.36 only reported 5 types of USP8 mutations in 6 of 17 Cushing's disease cases. We reported a total of 17 types of USP8 mutations in 75 of 120 Cushing's disease cases. We further identified three highly prevalent USP8 mutations, which account for the vast majority of mutated cases (77.61%). These data clearly define the spectrum of USP8 mutations. Furthermore, our study added valuable information which may provide guidance for the clinical practice in future: (1) Attributing to the larger sample size of the cohort, our study presents more clinical data to imply the clinical relevance of USP8 mutation. The major novel clinical finding is that USP8-mutated corticotrophin adenomas are significantly smaller in size but possess higher capacity to produce ACTH (P < 0.001), which clearly describes the clinical characteristics of the potential subjects who may benefit from targeted therapy developed from USP8 mutations. (2) We performed inhibitory functional experiments in primary human ACTH-secreting corticotrophin adenoma cells, which was a more genetically and clinically faithful cell model. For the first time, we showed that USP8 knockdown or gefitinib treatment significantly reduced ACTH secretion in primary USP8-mutated corticotrophin adenoma cells, but not in wild-type cells. These novel findings provide direct evidence that inhibition of USP8 and EGFR is promising for treating USP8-mutated corticotrophin adenoma. Taken together, the findings from our study and study by Reincke et al. will contribute to the development of novel therapies for Cushing's disease. New therapy targeting USP8 and/or EGFR might become the first-line treatment for USP8-mutated corticotrophin adenomas, especially for those surgically uncured and relapsed cases.
Materials and Methods
Patients were recruited at the department of neurosurgery at Huashan Hospital affiliated to Shanghai Medical College, Fudan University from 2003 to 2014 for this study. A total of 120 patients (99 women and 21 men; age range from 14 to 60) with Cushing's disease were included in this study. 12 randomly selected cases were used for initial exome sequencing, and the remaining 108 cases were used to study the prevalence of USP8 mutation (Supplementary information, Table S1). In addition, we analyzed 50 NF PAs, 50 GH-secreting PAs and 50 PRL-secreting PAs (Supplementary information, Table S1). Two normal pituitary tissues were obtained from cadaveric organ donations and tested to be suitable for immunohistochemical studies. All patients gave written informed consent, and the ethics committee at Huashan Hospital approved the study.
Cushing's disease was diagnosed using previously described and currently accepted criteria4. Briefly, the diagnosis of hypercortisolism was based on typical catabolic features (ie, facial plethora, purple striae, muscle atrophy of the limbs) and a combination of biochemical test results are as follows: increased awake midnight serum cortisol levels (> 7.5 μg/dl (> 207.3 nmol per liter)), elevated 24-h UFC (> 129.13 μg (> 356.9 nmol)) and nonsuppressible serum cortisol levels (> 5 μg/dl (> 138.2 nmol per liter)) after the administration of 1 mg of dexamethasone. The pituitary source of hypercortisolism was defined by increased morning plasma ACTH levels (> 20 pg/ml (> 4.4 pmol per liter)) and > 50% suppression of 24-h UFC in the classic high-dose dexamethasone-suppression test (HDST). Finally, contrast-enhanced T1-weighted MRI scanning was used to visualize adenomas. The presence of microadenomas (usually maximal diameter < 6 mm), which were undetected by MRI, was inferred by bilateral inferior petrosal sinus sampling (BIPSS). The final diagnosis of all Cushing's disease cases was histologically confirmed by two senior pathologists in blinded fashion. Of note, all surgically resected PAs from these patients are positive for ACTH staining, and ACTH immunostaining for 12 PAs used for whole-exome sequencing is shown in Supplementary information, Figure S9. The clinical details of 120 patients with Cushing's disease are provided in Supplementary information, Table S3. All reference ranges over the endocrine parameters in this study are provided in Supplementary information, Data S1.
Whole-exome sequencing and candidate gene validation
We performed exome sequencing of DNA from fresh tumor tissues of 12 ACTH-secreting PAs and matched blood samples on the HiSeq 2500 platform (Illumina). Exome sequencing libraries were enriched using the TruSeq™ Exome Enrichment 62 Mb Kit (Illumina). Sanger sequencing was performed to validate the candidate gene in 108 ACTH-secreting PAs and 150 other PA types. Detailed methods of DNA extraction, library preparation and variant detection are described in Supplementary information, Data S1.
DNA constructs and site-directed mutagenesis
Full-length wild-type human USP8 and 14-3-3ε expression plasmids were purchased from GE Dharmacon. cDNA encoding 14-3-3ε was cloned into pGEM2 (Promega) expression vectors. The USP8 encoding sequence was amplified and cloned into the T-easy vector (Promega), which was used for site-directed mutagenesis. The desired mutations in USP8 cDNA were introduced with the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). cDNA fragments encoding wild-type and mutant USP8 were subcloned into the Flag-tagged pcDH-EF-T2A-puromycin lentivirus expression vector (System Biosciences). The primers used in the mutagenesis are listed in Supplementary information, Table S4.
The statistical analysis was performed using SPSS version16.0 (SPSS Inc.), and graphs were prepared with Prism 6.0 (GraphPad) software. Pearson's χ2 test and the Mann-Whitney U-test were used for the categorical variables and the continuous variables, respectively. P < 0.05 was considered to be statistically significant.
Additional methods are provided in Supplementary information, Data S1.
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We specially thank Genetron Health (Beijing) Co., Ltd for assistance with genomic data analysis. We gratefully acknowledge Professor Zhengping Zhuang, Dr Lei Xiong, Professor Lu Xie, Dr Baofeng Lian, Professor Xingdang Liu and Professor Zhebao Wu for helpful discussions. We thank Professor Yuqing Wang, Mrs Qiuwei Song, Ms Yun Zhang, Mrs Yun Lu and Mr Qiongji Zhu for sample collection. We also thank Jingjing Zhu and Chao Li for their technical assistance with immunohistochemical staining. This study was supported by China Pituitary Adenoma Specialist Council (CPASC), the National High Technology Research and Development Program of China (863 program; 2014AA020611), the National Program for Support of Top-Notch Young Professionals, the National Natural Science Foundation of China (81172391), Program for New Century Excellent Talents in University (NCET-10-0356), Shanghai Rising-Star Tracking Program (12QH1400400) to YZ; the National Basic Research program of China (973 Program; 2015CB559100), the National High Technology Research and Development Program of China (863 program; 2012AA02A515), the National Natural Science Foundation of China (31325014, 81130022 and 81272302), Shanghai Jiao Tong University Liberal Arts and Sciences Cross-Disciplinary Project (13JCRZ02), Shanghai Key Laboratory of Psychotic Disorders (13dz2260500), “Shu Guang” project supported by Shanghai Municipal Education Commission and Shanghai Education Development Foundation (12SG17) to Y-Y S.
( Supplementary information is linked to the online version of the paper on the Cell Research website.)
Supplementary information, Table S1
Overview on the patients included in this genetic study and summary of USP8 mutational status of these patients. (PDF 112 kb)
Supplementary information, Table S2
Overview on mutations found in individual tumor sample upon exome sequencing and Sanger sequencing. (PDF 557 kb)
Supplementary information, Table S3
Clinical details of 120 patients with Cushing's disease. (PDF 305 kb)
Supplementary information, Table S4
Sequence of primers or shRNA against human USP8 (PDF 13 kb)
Supplementary information, Figure S1
Depth and coverage of whole-exome sequencing of target regions for paired tumor (T) and normal (N) DNA from 12 patients with Cushing's disease. (PDF 118 kb)
Supplementary information, Figure S2
Validation of three USP8 mutations detected by whole-exome sequencing. (PDF 165 kb)
Supplementary information, Figure S3
The allele frequencies of somatic mutations identified by whole-exome sequencing. (PDF 62 kb)
Supplementary information, Figure S4
Validation of other 14 types of USP8 mutations in 108 ACTH-secreting PAs by PCR and Sanger sequencing. (PDF 376 kb)
Supplementary information, Figure S5
Multi-alignment of partial amino acid sequence of USP8 in variety of species. (PDF 66 kb)
Supplementary information, Figure S6
Increased Erk1/2 phosphorylation in USP8-mutated PAs. (PDF 54 kb)
Supplementary information, Figure S7
Immunoblot analysis of EGFR and actin in primary USP8-mutated surgically resected cultured tumor cells infected with lentivirus expressing the indicated shRNA. (PDF 29 kb)
Supplementary information, Figure S8
Representative immunohistochemical staining of ErbB3 and C-Met in USP8-mutated ACTH-secreting PAs. (PDF 136 kb)
Supplementary information, Figure S9
Contrast-enhanced T1 weighted MRI and immunohistochemical staining of ACTH in 12 PAs used for whole-exome sequencing (WES). (PDF 312 kb)
Supplementary information, Data S1
Materials and Methods (PDF 91 kb)
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Cite this article
Ma, ZY., Song, ZJ., Chen, JH. et al. Recurrent gain-of-function USP8 mutations in Cushing's disease. Cell Res 25, 306–317 (2015). https://doi.org/10.1038/cr.2015.20
- Cushing's disease
- pituitary adenomas
- whole-exome sequencing
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