Development of the anterior-posterior axis is a self-organizing process in the absence of maternal cues in the mouse embryo

Development of the anterior-posterior axis is a self-organizing process in the absence of maternal cues in the mouse embryo


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Establishment of the anterior-posterior (AP) axis in the mouse embryo is crucial for proper induction of the germ layers. Despite this importance, it is unclear whether specification of the AP axis is an autonomous process or if it requires an embryonic-maternal interaction at the time of implantation. By embryonic day 5.5 (E5.5), the combined activities of Nodal, GDF3 and the Cripto co-receptor generate a proximal-distal gradient that establishes a signaling center at the distal tip of the embryothe distal visceral endoderm (DVE). This proximo-distal asymmetry is transformed into the AP axis as the DVE cells migrate. DVE cells express the Nodal antagonists, Lefty1 and Cer1, which specify the anterior identity of the underlying epiblast [1]. The expression of these key DVE markers is already triggered before implantation [2][3][4]. Cells that express Lefty1 and Cer1 in the late blastocyst contribute to DVE formation, together with cells that acquire de novo Cer1 and Lefty1 expression after implantation [3][4][5]. This pre-implantation expression of the Nodal antagonists led to the suggestion that the DVE, and therefore the AP axis, arise independently of any interaction between mother and embryo.
However, recently an alternative mechanism was proposed. By growing early post-implantation embryos in gels of different agarose concentrations Hiramatsu et al. [6] found that DVE is established only in stiffer gels, concluding that in vivo the maternal tissues should exert similar mechanical forces to enable DVE formation. Moreover, this biomechanical stress was reported to result in the transmigration of epiblast cells into the visceral endoderm (VE), which were suggested to generate the DVE at the distal tip. Therefore, two fundamentally different concepts about the mother's role in AP axis specification are currently considered.
To resolve whether establishment of the AP axis depends on mother-embryo interactions, or if it is an embryo-autonomous process, we performed a series of embryo culture and lineage tracing experiments. We used a Cer1-GFP line, previously established as a faithful DVE reporter [6,7], to monitor DVE formation and migration directly in living embryos.
First, we recovered embryos before implantation, at E3.5, and monitored their development and DVE formation through implantation stages in vitro on plastic plates, as we previously described [5,8,9]. Cer1-expressing cells became detectable after 1-1.5 day of culture and as development progressed they consolidated at the distal tip to establish the DVE (Supplementary information, Figure S1A, S1B and Movie S1). To capture the dynamics of DVE formation, we imaged pre-to post-implantation morphogenesis by time-lapse microscopy ( Figure  1A and Supplementary information, Movie S2). We analyzed 66 embryos and found that 87.8% established a Cer1-expression domain during the in vitro culture: in 58.3% it was positioned asymmetrically, and in 29.5% it remained located distally. Only in 12.2% of the embryos a Cer1-expression domain was not detectable (Supplementary information, Figure S1C and S1D). The migratory Cer1-expressing cells assembled into stringlike configurations, extending in a distal-to-proximal direction (Supplementary information, Figure S1E). We analyzed 39 divisions of Cer1-expressing cells in seven in vitro developing embryos, of which 69.9% were parallel to the direction of migration (Supplementary information, Figure S1E-S1G), suggesting that proliferating DVE cells become organized due to their pattern of divisions and migratory properties.
Next, we asked whether peri-implantation embryos (E5.0) could also establish the DVE in the absence of interactions with the mother. We analyzed the development of 59 E5.0 embryos and found that after 1 day of culture, 86% of them established a Cer1-expression domain: in 74.5% it was positioned asymmetrically and in 11.5%, it was located distally. Only 14% of the embryos lacked detectable Cer1 expression ( Figure 1B Figure S2K and Movie S3). As controls, we cultured 66 E5.5 embryos that had already established the DVE in utero by that stage in 87.9% of cases (Supplementary information, Figure S2D-S2F). Upon culture, the Cer1-expression domain moved asymmetrically in 81.3% of embryos (Supplementary information, Figure S2G, S2H and S2J). These observations were confirmed using another DVE reporter Lefty1(mVenus) [2]. Both E5.0 and E5.5 Lefty1(mVenus) embryos established asymmetric Lefty1 expression during 24 h of culture, without the requirement of external biomechanical pressure (Supplementary information, Figure S3A-S3C).
To exclude any possibility that interaction of the embryo with the substrate provides a mechanical stimulus leading to DVE formation, we cultured embryos free-floating in hanging drops of medium. We first cultured 40 E5.0 embryos, from which the majority (75.8%, n = 40) established a Cer1-expression domain and in 60.7% of cases this domain translocated anteriorly (Figure 1F, Supplementary information, Figure S4B and S4C). To determine whether the DVE is established in free-floating embryos from an even earlier stage, we cultured blastocysts in hanging drops. Again the majority of embryos (77.2%, n = 42) developed into egg cylinders that established a Cer1-expression domain and in 54.3% of embryos this domain translocated anteriorly (Supplementary information, Figure S4D-S4E).
Together, these experiments demonstrate that the DVE can form and migrate without a requirement of external spatial restriction on the embryo. These results are consistent in all experimental conditions irrespective of whether the embryos developed on a plastic substrate, in It has also been proposed that physical constriction of the embryo leads to transmigration of epiblast cells into VE to generate the DVE [6]. Since our results strongly indicate that DVE can form without any need of physical constriction, we next sought to determine whether the epiblast transmigration is essential for DVE formation. To this end, we used Rainbow transgenic mice [10] in combination with mice carrying a Sox2Cre transgene [11] to follow individual genetically labelled epiblast cells.
The first labelled epiblast cells in a color other than the default red were detected at E4.75 (n = 7; Figure 1G). While analyzing Rainbow-Sox2Cre embryos, we found that when the proamniotic cavity starts expansion (E5.25), a small proportion of embryos had cells of epiblast origin in the VE. We analyzed 33 embryos recovered at E5.25-E5.75 and found that 33% had epiblast-originating cells in VE ( Figure 1H and 1I). On average, two cells of epiblast-origin were present in the VE per embryo ( Figure 1J). Such cells in an embryo were the same color and usually in close proximity to each other, suggesting that they were sister cells. In two cases two non-adjacent cells of different colors were found in the VE (Figure  1Hd), raising the possibility that the epiblast can contribute to VE on more than one occasion in the same embryo. The position of epiblast-originating cells was not skewed to a particular site in the embryo: in three cases, epiblast-originating cells in the VE were in close proximity to the embryonic-extra-embryonic junction; in five cases, in the lateral part of the VE; and in four cases, at the distal tip ( Figure 1K (in vivo)). A similar situation was observed at E6.5, where 4 out of 16 embryos had epiblast-originating cells in VE (29%, Figure 1I). As with E5.25-E5.75 embryos, E6.5 embryos had an average of two epiblast-originating cells in the VE that were of the same color and always in the lateral part of the VE ( Figure  npg www.cell-research.com | Cell Research 1J). The presence of epiblast-originating cells in the VE suggests that epiblast cells transmigrate as the embryo develops. To test that, we filmed the in vitro development of Rainbow-Sox2Cre embryos recovered at E5.25 or E5.75 by time-lapse microscopy. In 14 filmed embryos, we found 6 in which epiblast cells transmigrated into the VE at random positions ( Figure 1K (post-IVC), Supplementary information, Figure S5A and S5B).
Finally, to determine whether the epiblast-to-VE transmigration could occur in the absence of any physical influence of the maternal environment, we filmed the in vitro development of blastocysts into egg cylinders (n = 14). As the pro-amniotic cavity started expansion, a single epiblast cell transmigrated into the VE in half of observed embryos ( Figure 1L, Supplementary information, Figure S5A and Movie S4). These transmigrated cells became embedded at different positions in the VE or excluded from the embryo and in none of the cases contributed to the DVE ( Figure 1K (pre-post-IVC) and 1L).
These results demonstrate that the epiblast-to-VE transmigration does take place, which is in agreement with the recent report [6]. However, the results we present here indicate that the epiblast-to-VE transmigration occurs not just at the distal tip, but in random positions along the egg cylinder, and that it is not required for DVE formation.
In a series of embryo culture experiments in different environmental conditions we show here that the AP axis in the mouse embryo can be specified in the absence of embryo-maternal interaction and without any external constraint. These findings contradict the suggestion that maternal tissues are essential to exert mechanical forces that enable DVE formation [6]. In the previous study, the authors report that it is necessary to encase embryos in an agarose gel in order to induce DVE formation in vitro. Moreover, gels containing less than 0.5% agarose were described as unable to support embryo elongation and DVE formation, contrary to our observations. How might the differences between these results arise? One possibility is the method of embryo culture. Whereas medium containing rat serum was used by Hiramatsu et al., here we culture embryos in a serum-free medium. Initially we attempted a similar embryo culture approach reported by Hiramatsu et al.; however we were unable to obtain a batch of rat serum of sufficient quality to support normal embryo growth (data not shown). To overcome this obstacle, we used the chemically defined IVC2 medium that we have recently established, which supports embryonic development beyond the blastocyst stage [8,9]. It is possible that the defined medium has an advantage over the serum-containing media as it excludes variability between batches of serum and the possibility of supply-ing growth factors and morphogens found in serum that might influence the outcome of the culture. Therefore, our results indicate that if satisfactory culture conditions are provided, the DVE can be specified outside the maternal environment in the absence of external pressure. Together, our results suggest that the processes by which the DVE forms and migrates to set up the AP axis are embryo-autonomous, illustrating the self-organizational properties of the mouse embryo.