Figure 2

From: Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses

Figure 2

MIR2911 targets various influenza viruses. (A) Prediction of the viral genome sequence targeted by MIR2911. (B) Validation of the predicted MIR2911 target sequence using a luciferase reporter assay (n = 3). (C) Western blot analysis of the PB2 and NS1 levels after transfecting HEK293T cells with the plasmids expressing PB2 or NS1, MIR2911 or ncRNA. (D) Western blot analysis of the mutant PB2 and NS1 levels after transfecting HEK293T cells with the plasmids expressing mutant PB2 or NS1, MIR2911 or ncRNA. (E, F) Anti-H1N1 effects of synthetic MIR2911 (E) and HS total RNA (F) in MDCK cells (MOI = 0.1, n = 6). (G, H) Effects of synthetic MIR2911 (G) and HS total RNA (H) on mutant H1N1 virus in MDCK cells (MOI = 0.1, n = 6). *P < 0.05; ***P < 0.001. All values are the mean ± SEM. P values were determined using Student's t-test.