Figure 1

From: Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses

Figure 1

MIR2911 is highly enriched in HS decoction and is delivered into mouse lungs. (A, B) The levels (sequencing reads) of plant miRNAs in HS (A) and HS decoction (B) detected by Illumina sequencing. (C) The concentrations of MIR2911 in HS and HS decoction determined by RT-qPCR. (D) Northern blotting analysis showing MIR2911 expression in 10 g of HS or 10 ml of HS decoction (final concentration: 0.2 g HS/ml). (E) MIR2911 kinetics in mouse plasma after administration of 500 μl of HS decoction (MIR2911 concentration: 0.12 nM) by gavage (0 h, n = 30; other time point, n = 6). (F) MIR2911 kinetics in mouse lung tissue after administration of 500 μl of HS decoction (MIR2911 concentration: 0.12 nM) by gavage (n = 5). (G, H) The MIR2911 level in mouse plasma (G) and lung tissue (H) 3 days after drinking HS decoction (MIR2911 concentration: 0.06 nM) (n = 5). (I, J) The MIR2911 kinetics in mouse plasma (I) and lung tissue (J) after administration of 100 pmol synthetic MIR2911 in 100 μl PBS by gavage (n = 5). (K) Fluorescently labeled MIR2911 in mouse lung tissue 3 h after administration of 2 nmol synthetic, fluorescently labeled MIR2911 by gavage (representative images are shown). Note that labeled MIR2911 (green dots) rapidly accumulated in mouse lungs. *P < 0.05; ***P < 0.001. All values are the mean ± SEM. P values were determined using Student's t-test.