Figure 5 | Cell Research

Figure 5

From: Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis

Figure 5

Mlkl is not involved in activation of NF-κB and MAPKs. (A) Wild-type and Mlkl−/− MEF cells were treated with TNF (20 ng/ml) for the indicated times, and then were analyzed by western blot for p-IκB, IκB, p-ERK, ERK, p-JNK, JNK, p-p38, p38, and β-actin (loading control). (B) BMDMs from wild-type and Mlkl knockout mice were treated with LPS (100 ng/ml) for different time periods as indicated, and then were analyzed by westen blot for p-IκB, IκB, p-ERK, ERK, p-JNK, JNK, p-p38, p38, and GAPDH (loading control). (C) Expression levels of cytokines in LPS-treated wild-type and Mlkl knockout bone marrow-derived macrophages were analyzed by real-time RT-PCR. (D) Expression levels of cytokines in LPS-treated wild-type and Mlkl knockout bone marrow-derived macrophages were analyzed by ELISA. Data are presented as mean ± SEM of triplicates. (E) Wild-type (n = 4) and Mlkl knockout mice (n = 4) at 8 weeks of age received 30 mg/kg LPS by intraperitoneal injection. The levels of IL-1β, and TNF-α in the serum after injection were measured at the indicated time points by ELISA.

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