Figure 4 | Cell Research

Figure 4

From: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

Figure 4

Genome-wide expression analysis of cells transfected with the CRISPR-on system. (A) The histogram showing distribution of Log2 fold changes of gene expression in sample transfected with dCas9VP160/sgTetO over dCas9VP160/sgTetO-mut control. (B) A histogram showing distribution of log2 fold changes of gene expression in cells transfected with dCas9VP160/sgIL1RN13 over dCas9VP160/sgTetO-mut control. Vertical line marks the fold change of the target gene IL1RN. (C) Column graph showing the Log2 fold changes of genes upregulated by at least 2-fold in cells transfected with dCas9VP160/sgTetO over dCas9VP160/sgTetO-mut. (D) Column graph showing the Log2 fold changes of genes upregulated by more than 2-fold in cells transfected with dCas9VP160/sgIL1RN13 over dCas9VP160/sgTetO-mut. Dotted line indicates the 2-fold cut-off.

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