Figure 2 | Cell Research

Figure 2

From: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

Figure 2

dCas9VP160 activates endogenous genes. (A) Protein architecture of dCas9VP160 compared to VP48. (B) Schematic of the human IL1RN promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of the sgRNAs. (C) Activation of human IL1RN expression in HEK293T cells. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (D) Schematic of the human SOX2 promoter region. Locations of TSS and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (E) Activation of SOX2. Cells were analyzed by qRT-PCR 2 days after transfection with dCas9VP160 and the indicated sgRNAs. (F) Schematic of the human OCT4 promoter region. Locations of transcription start site (TSS) and start codon (ATG) are indicated. Short lines with number indicate targeting sites of sgRNAs. (G) Activation of OCT4. Cells transfected with dCas9VP160 and the indicated sgRNAs were analyzed by qRT-PCR 2 days later. sgTetO-mut, negative control sgRNA. Error bars show SD among triplicates.

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