Abstract
The fresh water polyp Hydra belongs to the phylum Cnidaria, which diverged from the metazoan lineage before the appearance of bilaterians. In order to understand the evolution of apoptosis in metazoans, we have begun to elucidate the molecular cell death machinery in this model organism. Based on ESTs and the whole Hydra genome assembly, we have identified 15 caspases. We show that one is activated during apoptosis, four have characteristics of initiator caspases with N-terminal DED, CARD or DD domain and two undergo autoprocessing in vitro. In addition, we describe seven Bcl-2-like and two Bak-like proteins. For most of the Bcl-2 family proteins, we have observed mitochondrial localization. When expressed in mammalian cells, HyBak-like 1 and 2 strongly induced apoptosis. Six of the Bcl-2 family members inhibited apoptosis induced by camptothecin in mammalian cells with HyBcl-2-like 4 showing an especially strong protective effect. This protein also interacted with HyBak-like 1 in a yeast two-hybrid assay. Mutation of the conserved leucine in its BH3 domain abolished both the interaction with HyBak-like 1 and the anti-apoptotic effect. Moreover, we describe novel Hydra BH-3-only proteins. One of these interacted with Bcl-2-like 4 and induced apoptosis in mammalian cells. Our data indicate that the evolution of a complex network for cell death regulation arose at the earliest and simplest level of multicellular organization, where it exhibited a substantially higher level of complexity than in the protostome model organisms Caenorhabditis and Drosophila.
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Introduction
The mechanisms of programmed cell death in invertebrates have mainly been studied in Caenorhabditis elegans and Drosophila melanogaster. Comparison of the molecular pathways of apoptosis in invertebrates with the elaborate cell death machinery in vertebrates has shown that they are highly conserved throughout evolution. The major players are caspases, caspase inhibitors, members of the Bcl-2 family of pro- and anti-apoptotic proteins and adaptors of the Ced-4/APAF-1 type (reviewed in 1, 2, 3). Cell death is mediated extrinsically by members of the TNF family of ligands and their receptors, which rely on adaptor proteins that link activated receptors to intracellular cell death executioners (reviewed in 4).
Despite the high degree of conservation of these proteins, the level of complexity of the cell death pathways differs between Drosophila and Caenorhabditis. For example, extrinsic cell death pathways and caspase inhibitors of the IAP type have not been described in Caenorhabditis so far. Only one caspase, Ced-3, is involved in cell death in the nematode, whereas seven are present in Drosophila, including initiator and effector caspases. Similarly, differences exist in the Bcl-2 family of pro- and anti-apoptotic proteins. Caenorhabditis expresses the multidomain Bcl-2 homolog Ced-9 with a primarily anti-apoptotic function and the BH3-only proapoptotic protein Egl-1. In Drosophila, no functional anti-apoptotic Bcl-2 homolog has been found, although two homologs of multidomain Bcl-2 family members, Debcl and BUFFY, are present. Debcl has been shown to harbor a pro-apoptotic function, whereas BUFFY can apparently have anti-apoptotic characteristics in vivo in contrast to its ability to induce cell death when overexpressed ectopically in S2 insect cells 5.
In the face of such a diversity of apoptotic pathways in Caenorhabditis and Drosophila, it appears important to look for apoptotic networks in evolutionarily older metazoans. The phylum Cnidaria branched early from the main stem leading to bilaterian animals. Its members form two germ layers from which ectodermal and endodermal tissues are derived. They possess a simple nervous system constituting a nerve net across the whole animal without clearly defined central ganglia or brain-like structures. Caspase-dependent apoptosis has been described in the fresh water polyp Hydra, where it regulates cell numbers in response to feeding and is involved in oogenesis and spermatogenesis 6, 7, 8, 9, 10. In the colonial hydrozoan Hydractinia echinata, it is necessary for metamorphosis 11, 12. Recent genome sequencing projects have indicated the presence of large families of apoptotic proteins, including caspases, Bcl-2 family proteins and APAF-1 in cnidarians 13, 14, 15. However, their functional interactions and the roles they have in apoptosis are unknown.
In this study, we have focused on caspase and Bcl-2 family genes from Hydra magnipapillata. We have carried out functional studies to examine whether sequence homologies are paralleled by similarities in the function of apoptotic networks. Our results suggest that apoptotic pathways in cnidarians show almost the same functional complexity as in vertebrates.
Results
Caspases
A search of whole genome and EST sequences from Hydra has revealed 17 sequences with homology to caspases. With the exception of two (designated pseudo-caspases), all have putative active-site sequences with conserved histidine and cysteine residues 15. Two have been published previously (HvCasp3A and 3B 16) and have now been renamed as HyCaspA and HyCaspB 15. Nine of the Hydra caspase sequences have relatively long prodomains. A search for conserved protein-protein interaction motifs in these prodomains showed that two have CARD domains, one has a DED domain and, unusually for metazoan caspases, one has a DD domain. To further analyze Hydra caspases, we isolated cDNAs for the new genes HyCaspC and D and from all caspases with CARD, DED and DD domains. Figure 1 shows a schematic representation of the eight caspases that were investigated in these experiments. A review of all Hydra caspase sequences that have been identified in the Hydra genome has been published recently 15.
In the face of the complexity of caspase sequences in Hydra, we first analyzed whether some of them were responsible for caspase-3 activity in hydra extracts. Previous work using fluorogenic substrates had established that a DEVD-specific proteolytic activity is present in lysates of hydra cells in which apoptosis was induced 16. This activity could be labeled with the pan-caspase-specific active-site labeling reagent z-EK(biotin)D-AOMK. The activity also developed spontaneously in extracts of hydra cells incubated at room temperature for 1 to 2 h (data not shown). Active-site labeling was inhibited by the pan-caspase inhibitor z-VAD-FMK and the caspase-3 specific inhibitor DEVD-FMK, but not by the ICE-inhibitor YVAD-FMK (Figure 2A).
In order to establish the molecular identity of this activity, we purified the activated caspases responsible for cleaving the specific caspase-3 substrate DEVD-AMC from Hydra extracts. These extracts had been left at room temperature for 1 h and exhibited DEVD-AMC cleaving activity. After ion exchange chromatography and gel filtration, DEVD-AMC cleaving fractions were pooled. An antibody that had been raised against bacterially expressed recombinant HyCaspB (which recognizes both HyCaspB and HyCaspC because of their sequence similarity) stained a 38 kDa protein on western blot after SDS-PAGE, which corresponded in size with full-length HyCaspB and HyCaspC (Figure 2B, before). To isolate the catalytic fragment, caspase fractions were incubated with biotinyl-DEVD-CHO and affinity purified on streptavidin sepharose. A 28 kDa fragment was enriched and also stained with this antibody on a western blot after SDS-PAGE (Figure 2B, after). In contrast, antibody that had been raised against HyCaspA did not recognize this 28 kDa fragment (data not shown). The fragment had the same size as that of active-site-labeled proteins seen in extracts of apoptotic Hydra cells (Figure 2A). These data indicate that processed HyCaspB and/or C were at least in part responsible for the DEVD-specific caspase activity that is found in apoptotic Hydra cells, whereas HyCaspA was not.
We next investigated possible caspase activation cascades. In addition to the previously published HyCaspA and B, we cloned HyCaspC and D (HyCaspD has a very short prodomain of only eight amino acids), the two CARD-caspases, HyCARD1 and 2, the DED-caspase, HyDEDCasp, and the caspase with the DD domain, HyDDCasp, from Hydra vulgaris cDNA. We then expressed myc-tagged versions of these caspases in E. coli. For HyCaspA and HyDDCasp, we found evidence for autoprocessing and activation, as shown in Figure 3. Western blots of soluble extracts from bacteria expressing myc-tagged wild-type HyCaspA stained with anti-HyCaspA or anti-myc antibodies yielded three bands corresponding to full-length, unprocessed HyCaspA (PLS-myc), large subunit plus prodomain (PL) and the small subunit including the myc tag (S-myc) (Figure 3A and 3B). Expression of an active-site mutant (C211S) yielded only full-length unprocessed protein (PLS-myc). Deletion of the prodomain also blocked autoprocessing and yielded only the uncleaved product (LS-myc; Figure 3E). N-terminal sequencing of the small subunit (S-myc) indicated that autoprocessing occurred after aspartate in the sequence IRKDGIK (Figure 3G).
As expected, the 30 kDa fragment containing the large subunit could be labeled with the pan-caspase active-site label, FITC-VAD-FMK, and then detected with anti-FITC antibody after SDS-PAGE and western blot (Figure 3C). The active-site mutant (C211S) could not be labeled with this active-site reagent (data not shown). Together, these results clearly demonstrate that recombinant HyCaspA undergoes self-activation by autocatalytic cleavage and that this processing requires the prodomain of HyCaspA, although this prodomain has none of the known signatures for apoptotic caspases, such as CARD or DED domains.
In lysates from bacteria expressing HyDDCasp, only a small fraction of recombinant protein underwent autoprocessing, but this could be clearly documented with the active-site label FITC-VAD-FMK. The wild-type enzyme, but not the active-site mutant (C268S), yielded a 20 kDa band corresponding to the large subunit (Figure 3F).
To determine the substrate specificity of the HyCaspA and HyDDCasp, we performed competition experiments with unlabeled inhibitors. Figure 3C shows that the activity of HyCaspA was inhibited by the pan-caspase inhibitor z-VAD-FMK, but not by the caspase 3-specific inhibitor Ac-DEVD-CHO and the ICE-inhibitor Ac-YVAD-CHO. The HyDDCasp activity, on the other hand, was inhibited by Ac-YVAD-CHO, but not by Ac-DEVD-CHO or Ac-IETD-CHO (Figure 3D). Thus neither enzyme had the DEVD substrate specificity that was found in apoptotic extracts of hydra cells. Moreover, they both exhibited different substrate preferences, indicating that they cleave different substrates in vivo.
Similar experiments with HyCaspB, C, D, HyDEDCasp and HyCARD1 and 2 did not provide any evidence for autoprocessing or catalytic activity when expressed in E. coli (data not shown).
Bcl-2 family proteins
Caspase activation in metazoans involves permeabilization of the mitochondrial outer membrane, which leads to the release of pro-apoptotic factors. In mammals, these include cytochrome c, which participates in the formation of an apoptosome, including APAF-1 and procaspases. The permeability of the mitochondrial outer membrane is regulated by proteins of the Bcl-2 family, and hence, we searched the Hydra genome and EST sequences for Bcl-2 homologs. We identified nine Bcl-2 family members: two sequences show high similarity to vertebrate Bak proteins and were designated HyBak-like 1 and 2; the remaining sequences were designated HyBcl-2-like 15. Figure 4A shows alignments of the BH domains of the Hydra Bcl-2 family members. The BH1 and BH2 domains are very well conserved and show high sequence similarity with BH1 and BH2 domains from human Bcl-2 and human Bax. The BH3 domains, in contrast, are not well conserved, except for the presence of a conserved leucine residue in all sequences. Moreover, all Hydra Bcl-2-like and Bak-like proteins have a C-terminal transmembrane domain (Figure 4B).
All Hydra bcl-2 and bak genes have a conserved intron, splitting the coding region of the BH2 domain (Figure 4B). This intron is also found in human and invertebrate bcl-2 genes 17, supporting the idea that the Hydra genes are orthologs of the mammalian bcl-2 genes. Additional introns are found in the gene encoding HyBcl-2-like 7.
In order to understand the function of Bcl-2 proteins in Hydra, we analyzed their intracellular localization by expressing GFP-HyBcl-2-like and GFP-HyBak-like proteins in Hydra cells (Figure 5). Most of the Bcl-2-like proteins and both Bak-like proteins were localized to mitochondria. Deletion of the C-terminal transmembrane domain from HyBcl-2-like 1 blocked the association with mitochondria in Hydra (data not shown).
HyBcl-2-like 5, in comparison, localized to the cytoplasm and the nucleus in addition to mitochondria (Figure 5B), while HyBcl-2-like 7 localized to ER-like structures in the cytoplasm and not to mitochondria. This is shown by comparison of its localization with that of an RFP-tagged Hydra Bax inhibitor 1, a highly conserved integral membrane protein of the ER (see Materials and Methods) (Figure 5C).
We next expressed the Hydra Bcl-2 proteins in human HEK293T cells in order to test their effects on apoptosis. Apoptotic cells were scored by staining with the DNA dye TO-PRO-3 (see Figure 6A) and counting the percentage of apoptotic cells, in relation to all GFP-expressing cells. The result is shown in Figure 6B. Both HyBak-like proteins strongly induced apoptosis in this assay, as did human caspase-8, which was used as a control. Mutation (L265E) of the conserved leucine residue in the BH3 domain of HyBak-like 1 abolished this pro-apoptotic effect. While most of the remaining Hydra Bcl-2-like proteins had no effect when transfected into HEK293T cells, HyBcl-2-like 5 did exhibit a weak pro-apoptotic effect.
To analyze the anti-apoptotic effect of Hydra Bcl-2 family members, we transfected HEK293T cells with plasmids encoding Hydra Bcl-2 family proteins and then induced apoptosis with the topoisomerase inhibitor camptothecin. Except for HyBcl-2-like 5 (as expected because of its pro-apoptotic effect), all HyBcl-2-like proteins showed some protective effect. HyBcl-2-like 4 showed the strongest inhibition of camptothecin-induced apoptosis. Mutation (L256E) of the conserved leucine residue in the BH3 domain of HyBcl-2-like 4 abolished the protective effect (Figure 6C).
In addition, we analyzed interactions between Bcl-2 family members in a yeast two-hybrid system. The readout for interaction of two proteins in this system was growth of yeast on minimal medium lacking leucine and expression of a GFP reporter. Table 1 shows that Bcl-2-like 4 was capable of interacting with itself, with HyBak-like 1 and with human Bcl-xL. When the leucine residue in the BH3 domain of Bcl-2-like 4 was mutated (L256E), these interactions were abolished. All the other Hydra Bcl-2 family members failed to show interactions in the yeast two-hybrid system (Table 1), although they were expressed in yeast at similar levels based on protein detection with antibodies on western blots (data not shown).
BH3-only proteins
In view of the functional conservation of Hydra Bcl-2 family proteins in regulating apoptosis, we searched the Hydra genome for genes encoding BH3-only proteins, which might act to induce apoptosis in vivo. We carried out a profiled search for BH3 domains using structural and sequence motifs from known BH3-only proteins as a template 18. This yielded four Hydra gene models encoding novel proteins with conserved BH3 domains. They were designated as HyBH3-only 1, 2, 3 and 4 (Figure 7). Three of the gene models were validated by RT-PCR and cDNA cloning (these are printed in bold in Figure 7). Hma2.224514 codes for a short 83 amino acid protein with the BH3 domain followed by a transmembrane domain (HyBH3-only4). This domain structure is similar to the structure of human Hrk-1, although there is no further sequence homology outside these two domains 19. In order to test whether these Hydra BH3-only proteins could induce apoptosis, we expressed them in human HEK293T cells and scored apoptotic cells. Figure 8A shows four cells expressing GFP-HyBH3-only 2. Three cells in the left hand panel have rounded up and DNA staining indicates that they have apoptotic nuclei. By comparison, the cell on the right hand panel is not apoptotic. Figure 8B shows the percentage of apoptotic cells for each of the Hydra BH3-only proteins transfected into HEK293T cells. Only HyBH3-only 2 induced apoptosis in this system.
The apoptosis-inducing HyBH3-only 2 was then tested in the yeast two-hybrid system for interaction with the anti-apoptotic HyBcl-2-like 4 and the pro-apoptotic HyBak-like 1 proteins. As shown in Table 2, HyBH3-only 2 interacted strongly with HyBcl-2-like 4 but not with HyBak-like 1. By comparison, HyBH3-only4 did not interact with either HyBcl2-like 4 or HyBak-like 1.
Discussion
While genome searches in several lower metazoans have revealed that early nonbilaterian animals possess a large number of caspase and Bcl-2 family genes, there has been very little experimental evidence showing that these genes were involved in apoptosis. In contrast to this earlier work, our results show that these protein families, which are also expanded in Hydra, functionally participate in apoptotic pathways. Figure 9 summarizes the components of the apoptotic toolkit in Hydra, in comparison with apoptotic proteins from Homo sapiens. The similarity is striking and emphasizes the fact that complex apoptotic pathways evolved early in metazoan evolution and that they may have contributed significantly to stabilizing the multicellular state.
Caspases and caspase activation
We purified the DEVD-specific caspase activity from apoptotic extracts of hydra and showed, with the help of antibodies and specific substrates, that it corresponds to HyCaspB/C (Figure 2). Based on protein structure, these caspases appear to be executioner caspases and they are not autocatalytically activated when expressed in E. coli. This raises the question of how they become activated. Based on sequence homology, there are several potential initiator caspases in the Hydra genome that have CARD, DED and DD motifs in their prodomains. Most of them did not show autoactivation when expressed in bacteria; hence, we cannot yet define them as initiator caspases. However, they may require adaptor proteins for effective autoactivation. Two such activators, an APAF1 homolog with a CARD domain and a FADD homolog with a DED domain 15, are encoded in the Hydra genome and could fulfill this role. However, since caspase activation in our apoptotic extracts is quite fast, we have so far been unable to define a role for APAF1 or FADD.
Although we could not demonstrate autoactivation with CARD-caspase and DED-caspase, we did observe autoactivation in bacterial extracts with HyCaspA and with HyDDCasp (Figure 3). These two Hydra caspases have unusual prodomains. HyCaspA has a prodomain for which no homologs can be found in other caspases. However, it enabled autoprocessing and activation of HyCaspA, giving rise to a caspase with unusual substrate specificity. HyCaspA was inhibited with the pan-caspase inhibitor z-VAD-FMK, but not with any of the tetrapeptide inhibitors tested. Its autoprocessing site was C-terminal of the amino acids IRKD, consistent with the unusual substrate specificity of this caspase. The second caspase showing autoactivation has a DD-motif in its prodomain that has so far only been described in prodomains of paracaspases 20. Its caspase activity could be inhibited with Ac-YVAD-CHO, which suggests that it has a substrate specificity similar to human ICE (caspase 1) 21. Neither of these caspases appeared to be active in apoptotic cell lysates from hydra.
Hydra genome analysis has provided evidence for the presence of genes encoding components of the extrinsic pathway of apoptosis induction, which could participate in the activation of HyDEDCasp. These include a Hydra FADD homolog and a gene encoding a putative death receptor. This gene was cloned and shown to have two extracellular TNF-receptor domains and an intracellular DD domain, with high sequence conservation to vertebrate p75 NGF-receptors 15. Which ligands activate this receptor and what role it plays in Hydra biology is presently completely unclear. However, it should be noted that during oogenesis the developing oocyte initiates massive apoptosis in surrounding nurse cells and subsequently phagocytizes the nurse cell corpses 22. Thus the oocyte may be the source of a death ligand.
Extrinsic pathways for apoptosis induction via TNF-receptors, FADD-like adaptors and caspase-8 have so far not been documented in invertebrates. TNF-receptors have not been found in Caenorhabditis, nor have FADD homologs. In Drosophila, the TNF-receptor homolog Wengen does trigger cell death. However, it lacks an intracellular DD domain, and apoptosis is not dependent on the Drosophila caspase-8 homolog DREDD, but rather works through Drosophila DRONC (Caspase-9) and DARK (APAF1) 23, 24, 25.
Regulation of apoptosis in Hydra
The present experiments demonstrate the occurrence of Bcl-2 and Bak homologs in Hydra. Although it was not possible to test the function of these proteins in Hydra directly, expression of the proteins in mammalian cells indicated that several are functional homologs of their mammalian counterparts. Bcl-2-like 4 strongly inhibited camptothecin-induced apoptosis and this inhibition was blocked by mutation of the BH3 domain (Figure 6C). In comparison, HyBak-like 1 strongly induced apoptosis in HEK293T cells (Figure 6B) and interacted with Bcl-2-like 4 in the yeast two-hybrid assay (Table 1). Finally, HyBH3-only 2 also induced apoptosis in mammalian cells and interacted with HyBcl-2-like 4 in the yeast two-hybrid assay. Together, these results suggest that the Hydra Bcl-2 proteins have functional properties, which would permit them to regulate apoptosis in Hydra. This could potentially occur in a similar manner to mammalian cells, where BH3-only proteins induce apoptosis by interacting with anti-apoptotic Bcl-2 proteins and thus relieve the repression of proapoptotic multidomain Bcl-2 family proteins 26. These results constitute the first functional data that Bcl-2 family members in pre-bilaterian metazoans actually have the expected biochemical and cellular properties.
The diversity of the Bcl-2 family in mammals includes various biochemical characteristics, subcellular localizations and tissue-specific expression patterns. For instance, some human BH3-only proteins interact with all members of the anti-apoptotic Bcl-2 family, while others only interact with some. Binding affinities of BH3 domains for different family members also greatly vary. Moreover, BH3-only proteins can be induced by different stimuli such as death receptor signaling, growth factor deprivation and DNA damage. To induce apoptosis some are released from the ER or from the cytoskeleton (recently reviewed in 27). Our experimental data on the large Bcl-2 family in Hydra now suggest that the observed diversity could probably fulfill similar functions. The yeast two-hybrid experiments indicate that Hydra Bcl-2 family members have different binding affinities for each other or need different conditions for their interactions. Moreover, they are localized to different cellular compartments. Future experiments will show whether they are also expressed in a tissue-specific manner.
In summary, our data indicate that complex apoptotic pathways arose early in the evolution of multicellular animals. Raff 28 has proposed that apoptosis in animals is a means for social control between cell populations fulfilling different functions in the organism. Apoptotic mechanisms therefore evolved together with the evolution of such populations in multicellular animals 29.
Strikingly, this evolution gave rise to complex regulatory pathways right from the beginning. This complexity is also reflected in the variety of different roles for apoptosis in Hydra and other cnidarians including cell number regulation, gametogenesis and metamorphosis.
Materials and Methods
Hydra culture
Hydra were cultured in Hydra medium (0.1 mM KCl, 1 mM NaCl, 0.1 mM MgSO4, 1 mM Tris-HCl, 1 mM CaCl2 (pH 7.6)) at 18 °C. They were fed daily with freshly hatched Artemia nauplii larvae and washed after 6-8 h to remove undigested material, which was expelled into the medium.
Preparation of Hydra lysate
Animals were washed in Hydra medium and in buffer A (25 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM EGTA, protease inhibitors leupeptin, pepstatin A and aprotinin at 10 μg/ml each and 10 mM Pefabloc, Roche), homogenized by extrusion through a fine needle in buffer A and frozen at −80 °C. After thawing, samples were sonicated three times for 10 s and centrifuged for 15 min at 12 000× g. The supernatant was used for active-site labeling with z-EK(biotin)D-AOMK or FITC-VAD-FMK.
Purification of DEVD-specific Hydra caspase
Lysates were prepared from 10 000 animals and incubated at room temperature for 1 h to induce caspase activity, which was measured with DEVD-AMC 16. The extract was loaded on a HiPrep16/10 DEAE-anion exchange column (Amersham Biosciences) equilibrated with 20 mM Tris, 0.1% CHAPS, 1 mM DTT (pH 7.8) (buffer A), the column was washed with buffer A and proteins were eluted with a NaCl gradient from 0 to 0.5 M NaCl. Caspase activity was high in fractions eluted at 0.2 M NaCl. Active fractions were collected, concentrated and loaded onto HiLoad 16/10 Superdex 200, prep-grade gel filtration column (Amersham Biosciences) equilibrated with buffer A with 0.2 M NaCl. The column was eluted with buffer A with 0.2 M NaCl. Active fractions were again collected, concentrated and incubated with 8 μM biotinyl-DEVD-CHO in 20 mM Tris/HCl, 0.1% CHAPS, 10 mM DTT, 5 mM MgCl2 (pH 7.8). They were then loaded on a HiTrap-streptavidin column (Amersham Biosciences). The column was washed with buffer A with 0.15 M NaCl and then eluted with 2 mM biotin in this buffer. All eluted fractions were analyzed in SDS-PAGE, western blotted and stained with anti-HyCaspB/C antibody.
Caspase activity assay with z-EK(biotin)D-AOMK or FITC-VAD-FMK
Bacterial soluble extract was incubated for 2 h at room temperature in assay buffer (25 mM HEPES (pH 7.5), 0.1 % (w/v) CHAPS, 10 mM DTT, 10 μg/ml pepstatin A, 10 μg/ml aprotinin, 10 μg/ml leupeptin, 10 mM pefabloc) with 1 μM of the active-site labeling reagents z-EK(biotin)D-AOMK (Peptide Institute Inc, Japan) or FITC-VAD-FMK (Alexis). To test for caspase inhibition, the sample was preincubated for half an hour with 1 μM of the indicated inhibitors before adding the active-site labeling reagent. After incubation, SDS sample buffer was added and SDS-PAGE and western blot analysis was performed. Active fragments were detected by streptavidin-peroxidase for z-EK(biotin)D-AOMK substrate or by the monoclonal mouse anti-FITC-antibody (kind gift from Burkhard Micheel, University of Potsdam, Germany) for FITC-substrate.
Generation of antibodies
Affinity-purified denatured His6-myc-tagged HyCaspA and HyCaspB were used for chicken immunization (Davids, Regensburg, Germany). An IgY preparation was used for the experiments. The antibodies were shown to recognize their respective bacterially expressed caspase antigens on western blots.
Cloning
HyBcl-2-like 1-7, HyBak-like 1 and 2 and HyBH3-only 2, 3 and 4 were first amplified using Hydra cDNA as template. Primers were designed for amplification of the coding sequences, as deduced from gene models and EST sequences. The PCR products were cloned into pCR®2.1-TOPO using the TOPO TA-cloning Kit (Invitrogen), followed by sequencing. The 3′ end of HyBcl-2-like 7 was obtained by RACE reactions using the GeneRacer Kit (Invitrogen). For the fluorescent fusion proteins GFP-HyBcl-2-like 1-7 and GFP-HyBak-like 1 and 2, the corresponding sequences were cloned into the vector hoT G 30 to the 3′ end of the sequence encoding the fluorescent protein, using the EcoRI site. For yeast two-hybrid assays, the coding sequences of the HyBcl-2 family genes were cloned into the EcoRI and XhoI sites of the bait vector pEG202 or the prey vector pJG4-5 (MoBiTec). For expression in mammalian cells, HyBcl-2 family genes were cloned into the vector pEGFP (Clontech), allowing expression of N-terminal GFP-tagged HyBcl-2 family proteins.
Transfection of hydra cells
Gold particles (1.0 μm, BioRad) were coated with plasmid DNA according to the instructions of the manufacturer. They were introduced into hydra cells with the Helios gene gun system (BioRad), as previously described 30.
Immunofluorescence
Animals were relaxed for 2 min in 2% urethane, fixed with 2% paraformaldehyde for 1 h at room temperature, washed with PBS, permeabilized (0.5% Triton/PBS) and blocked (0.1% Triton, 1% BSA/PBS). Incubation with the primary antibody (for mitochondria: anti-OxPhos Complex V subunit α-mouse IgG2b, monoclonal Ab 7H10 (Invitrogen)) in blocking solution was carried out overnight at 4 °C. The secondary antibody (Cy3 AffiniPure sheep anti-mouse IgG (H+L); Dianova/Jackson Immuno Research) was incubated for 2 h at room temperature followed by washing and DAPI/TO-PRO-3 staining. Finally, the animals were mounted on slides with Vectashield mounting medium (Alexis Biochemicals).
Hydra ER-labeling
RFP-HyBax-inhibitor 1 was used as a marker for Hydra ER. Bax inhibitor 1 is an integral ER-membrane protein with high homology between mammals and Hydra 31. By searching the Hydra genome (at http://hydrazome.metazome.net/cgi-bin/gbrowse/hydra), we found a gene model for the Hydra homolog (hma1.130444) and cloned this sequence from Hydra cDNA. We then inserted it into the vector hoTRed 32 for expression in Hydra cells. When expressed in mammalian cells, the HyBax inhibitor 1 co-localized with calnexin (data not shown) and is therefore a specific marker for ER.
Confocal laser scanning microscopy
Optical serial sections were acquired with a confocal laser scanning microscope (TCS SP1, Leica Microsystems, Heidelberg, Germany) equipped with an oil immersion plan-apochromatic ×100/1.4 NA objective. The fluorochromes were scanned sequentially. EGFP was excited with a 488-nm argon laser line and Cy3 with a 568-nm krypton laser line using an excitation beam splitter TD 488/568/633. Emissions were recorded between 520-540 and 575-625 nm, respectively. The gray-scale single channel images were overlaid to an RGB image assigning a false color to each channel and then reassembled using Adobe Photoshop 7.0 software (Adobe Systems, USA).
Detection and induction of apoptosis in mammalian cells
Human embryonic kidney cells (293T cells) were cultured in DMEM (Sigma) supplemented with 10% FCS and 5% pen/strep. 293T cells were transfected using PEI (pH 7.0). Cells were fixed and counterstained with DAPI 24 h later. The nuclei of GFP-expressing cells were microscopically assayed for apoptotic morphology and the percentage of apoptotic nuclei was counted. To induce apoptosis, cells were treated with 10 μm camptothecin for 24 h.
Yeast strains, media and transformation
Standard genetic techniques were used for growth and manipulation of yeast strains.
S. cerevisiae strain EGY 48 (MATα, trp 1, his 3, ura 3, leu2::2/4/6 LECAop-LEU2) was used. Transformation of yeast was carried with lithium acetate; cells were grown in complete minimal medium lacking uracil, tryptophane, histidine or leucine to select for the presence of various plasmids. Interaction studies, including positive and negative controls, were performed according to the manufacturers instructions (MoBiTec (2004) Grow'n'Glow: The GFP Two-Hybrid System Product Information and Protocols Handbook).
Software and accession numbers
ClustalW2 und Clustal X (http://www.ebi.ac.uk/Tools/clustalw2/index.html) were used to generate alignments. Search for genes was performed using the Hydra genome server (http://hydrazome.metazome.net/cgi-bin/gbrowse/hydra/). BH3-only proteins were searched using the software HHsearch. A sequence context-specific profile for homology searching was used.
Accession numbers for human proteins used in alignments are: NP_000624 for human Bcl-2; NP_001179 for human Bak and NP_620116 for human Bax. Members of the Bcl-2 protein family in Hydra are accessible by the numbers EF104646 for HyBcl-2-like 1, EF104647 for HyBcl-2-like 2, EU035765 for HyBcl-2-like 3, EU035764 for HyBcl-2-like 4, EU035763 for HyBcl-2-like 5, EU035762 for HyBcl-2-like 6, EU035761 for HyBcl-2-like 7, EF104645 for HyBak-like 1 and EU035760 for HyBak-like 2.
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Acknowledgements
We thank Burkhard Micheel (Potsdam University) for the anti-FITC antibody, Rainer Deutzmann (Regensburg University) for sequencing the HyCaspA cleavage site and the DFG for support (BO1448-2).
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Lasi, M., Pauly, B., Schmidt, N. et al. The molecular cell death machinery in the simple cnidarian Hydra includes an expanded caspase family and pro- and anti-apoptotic Bcl-2 proteins. Cell Res 20, 812–825 (2010). https://doi.org/10.1038/cr.2010.66
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DOI: https://doi.org/10.1038/cr.2010.66