In quorum sensing (QS) process, bacteria regulate gene expression by utilizing small signaling molecules called autoinducers in response to a variety of environmental cues. Autoinducer 2 (AI-2), a QS signaling molecule proposed to be involved in interspecies communication, is produced by many species of gram-negative and gram-positive bacteria. In Escherichia coli and Salmonella typhimurium, the extracellular AI-2 is imported into the cell by a transporter encoded by the lsr operon. Upstream of the lsr operon, there is a divergently transcribed gene encoding LsrR, which was reported previously to repress the transcription of the lsr operon and itself. Here, we have demonstrated for the first time that LsrR represses the transcription of the lsr operon and itself by directly binding to their promoters using gel shift and DNase I footprinting assays. The β-galactosidase reporter assays further suggest that two motifs in both the lsrR and lsrA promoter regions are crucial for the LsrR binding. Furthermore, in agreement with the conclusion that phosphorylated AI-2 can relieve the repression of LsrR in previous studies, our data show that phospho-AI-2 renders LsrR unable to bind to its own promoter in vitro.
Quorum sensing (QS) is a process of cell-cell communication that bacteria use to regulate specific physiological functions in response to the fluctuations of the population density in a community 1, 2. QS bacteria secrete small, diffusible signaling molecules called autoinducers that accumulate in the external environment. When the concentration of the autoinducers reaches a threshold, an alteration of gene expression is induced, allowing the bacteria to adopt behaviors that are only productive when the bacteria are working together as a group 3, 4. Among many different mechanisms for QS by bacteria, one is shared by both gram-negative and gram-positive bacteria, involving the production of autoinducer 2 (AI-2) 5, 6, 7, 8, 9, 10.
AI-2 QS system was first identified in the marine bacterium Vibrio harveyi as a part of a complex multi-layered QS system responsible for regulating bioluminescence and other virulence-associated traits 4, 11. Subsequently, AI-2 production was verified in a large number of species and the AI-2 QS system was recognized to be involved in the regulation of a range of functions in diverse bacteria 6, 7, 10, 12, 13, 14. In contrast to other autoinducers that are specific for a narrow range of organisms, the widely conserved AI-2 has been hypothesized to be a universal language for interspecies communication 6, 9, 10, 13, 15. In every case, AI-2 is synthesized by LuxS, which functions in the pathway for metabolism of S-adenosylmethionine (SAM), a major cellular methyl donor. In a metabolic pathway known as the activated methyl cycle, SAM is metabolized to S-adenosylhomocysteine, which is subsequently converted to adenine, homocysteine, and 4,5-dihydroxy-2,3-pentanedione (DPD, the precursor of AI-2) by the sequential action of the enzymes Pfs and LuxS 10, 16. DPD is a highly reactive product that can rearrange and undergo additional reactions, suggesting that distinct but related molecules derived from DPD may be the signals that different bacterial species recognize as AI-2. Experimental evidence showed that in V. harveyi, AI-2 is (2S, 4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran-borate (S-THMF-borate); and in E. coli and S. typhimurium, AI-2 is (2R, 4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF) 10, 16, 17, 18, 19, 20, 21, 22.
Although the pathway for AI-2 production is widely conserved among bacteria, the mechanisms of AI-2 detection and signal transduction are specific and have only been established in a few species such as V. harveyi, Vibrio cholerae, S. typhimurium, and E. coli. In V. harveyi, AI-2 detection and transduction rely on a two-component system similar to that of the QS system in gram-positive bacteria 11. Boron-AI-2 is recognized by a soluble periplasmic protein that interacts with cytoplasmic response regulator proteins, thus triggering a sensor-kinase couple to finally control the expression of the luciferase structural operon (luxCDABE) 4, 21, 23, 24.
In E. coli and S. typhimurium, AI-2 is required to be imported into the cell for gene expression control. In E. coli and S. typhimurium, extracellular AI-2 accumulates in the exponential phase, but decreases drastically upon entry into the stationary phase. The rapid disappearance of AI-2 is due to its import by the lsr transporter 19, 25, 26, 27, 28. In E. coli, the lsr operon is comprised of six genes lsrACDBFG (Figure 1). The first four genes, lsrACDB, encode components of the ATP-binding cassette transporter, and the remaining two, lsrFG, are proposed to be required for the modification of AI-2 following internalization 28, 29.
The regulatory network for AI-2 uptake is comprised of two other important components, lsrR and lsrK, both of which are located adjacent, but divergently transcribed from the lsr operon (Figure 1). LsrR is the repressor of the lsr operon and itself. LsrK is a kinase responsible for converting AI-2 to phospho-AI-2, which is required for relieving LsrR repression. Functions of LsrR and LsrK can be partially revealed using lsrK and lsrR mutants. In lsrR mutants, the LsrACDB transporter is produced, and extracellular AI-2 is continuously imported into the cell (cytoplasmic AI-2). In lsrK mutants, the expression of lsr is repressed, and AI-2 remains in the supernatant (extracellular AI-2) 25, 26, 27, 28, 30, 31. Since LsrR contains a helix-turn-helix (HTH) DNA-binding domain, it was hypothesized that LsrR represses the expression of lsr operon and itself by binding to their promoter regions 26, 29, 32. However, no evidence of their direct binding has been shown yet. It has also been postulated that phospho-AI-2 binds to LsrR and inactivates it to derepress the transcription of lsr 25, 26. Although a previous study has revealed that phospho-AI-2 can bind to LsrR directly using in vitro assays 29, whether it is the same case in vivo and the detailed mechanism by which phospho-AI-2 derepresses the lsr transcription still remain obscure. In addition, it is interesting that another previous study has suggested that LsrR and LsrK may serve as global regulators working on genes other than the lsr operon, and hypothesized that LsrR is a QS regulator that can regulate the transcription of a variety of genes after binding to phospho-AI-2 32. Taking all these into account, it is important to explore the precise LsrR-binding mode.
In this study, we provide evidence for direct LsrR binding to the lsr promoter and the promoter of its own by gel shift assays. DNase I footprinting assays show that LsrR binds to a region between −20 and −49 with respect to the lsrR promoter. In addition, gel shift data also show that phospho-AI-2 prevents LsrR and the lsrR promoter from forming a complex, providing in vitro evidence that phospo-AI-2 can alleviate the LsrR-mediated repression.
LsrR binds to lsrA and lsrR promoters
LsrR has been revealed to be able to repress transcription from the lsrA and lsrR promoters, with deletion of lsrR resulting in maximal transcription of the lsr operon and the lsrR gene in S. typhimurium and E. coli 26, 31. Since LsrR contains a predicted HTH DNA-binding domain, it has been suggested to repress the transcription of the lsr operon and itself by directly binding to their promoters. In order to determine whether LsrR can bind to the lsrA and lsrR promoter regions, we carried out gel shift assays. His6-tagged LsrR was overexpressed in E. coli BL21 (DE3) and purified using Ni-NTA affinity resin. Two DNA fragments p-lsrR (119 bp from −1 to −119 of the lsrR translation start site) and p-lsrA (129 bp from −1 to −129 of the lsrA translation start site) were PCR-amplified and DIG-labeled (Figure 2A) for use as the probes. Results show that LsrR specifically binds to both lsrA and lsrR promoter regions. As shown in Figure 2B, from lane 2 to 5, two bands are observed. The lower one is the free labeled probes present in molar excess, and the higher one represents a protein-DNA complex. As the amount of added LsrR protein increased from 5 to 40 pmol (the final concentration was from 0.5 to 4 μM), the amount of the complex formed increased and the amount of free probes decreased correspondingly. For control, no LsrR was added in lane 1 and 10-fold unlabeled fragments were used as specific competitors in lane 6, and no protein-DNA complex was formed under these two conditions. We also used a random DNA sequence as the probe for the negative control, and the results show that LsrR cannot bind to this random fragment (data not shown). These data indicate that LsrR can specifically bind to the lsrA and lsrR promoter regions in vitro.
Phosphorylated AI-2 causes LsrR to relieve its binding to the promoters in vitro
In S. typhimurium and E. coli, LsrK can phosphorylate AI-2, and phospho-AI-2 is thought to be the signal molecule that activates transcription of the lsr operon via inactivation of LsrR 25. A recent study showed that phospho-AI-2 can directly bind to LsrR in vitro 29. However, it remains unclear how phospho-AI-2 relieves the LsrR repression. In order to explore whether phospho-AI-2 affects LsrR binding to the lsrR promoter in vitro, a series of gel shift assays were performed.
In vitro phosphorylation of the chemically synthesized AI-2 by LsrK was performed as described previously 25, 29. A full conversion of AI-2 to phospho-AI-2 was confirmed by high performance liquid chromatography (HPLC) assays. The quantity of phosphorylated AI-2 in the final preparation is almost the same as that of AI-2 we utilized before the start of the reaction, indicating that the conversion ratio is nearly 100%. The phospho-AI-2 was size-fractionated by using an ultrafiltration column to remove the LsrK protein. Gel shift assays were then performed with the purified LsrR protein and a molar excess of labeled DNA fragment from the lsrR promoter. Phospho-AI-2 inhibits the binding of LsrR to the lsrR promoter in a dose dependent manner, while AI-2 has no such effect (Figure 3A). From lane 2 to 5, the amount of the LsrR protein was equivalent, and when different amounts of AI-2 (the final concentration was increased from 0 to 390 μM) were added in the reaction mixtures, no reduction of the DNA-protein complex was observed. By contrast, from lane 7 to 10, there are apparent reductions of the complexes with the addition of the different amounts of phospho-AI-2 (the final concentration of phospho-AI-2 was from 0 to 390 μM).
Since ADP exists as one of the products in the phospho-AI-2 preparation, and there may be residual ATP that was not converted to ADP during the in vitro phosphorylation reaction, we incubated ATP and ADP, respectively, with the LsrR protein and the DIG-labeled p-lsrR fragments under the same conditions. Our results show that ADP and ATP do not affect the binding of LsrR to the promoter regions (Figure 3B), ruling out the possibility that the above phenomenon was due to the presence of ADP or ATP. In summary, phospho-AI-2 renders LsrR unable to bind to the lsrR promoter in in vitro assays.
Identification of the LsrR-binding sites in the lsrR and lsrA promoters
To further identify the precise LsrR-binding sequence, a DNase I footprinting was performed. A fragment incorporating the +51 to −119 region relative to the translation start site of the lsrR gene was fluorescently 5′-labeled with 6-carboxyfluorescein (6-FAM), and incubated with increasing amounts of His6-LsrR before being treated with DNase I. The digestion patterns were examined by capillary electrophoresis (CE) using a 3730XL automated sequencer (Applied Biosystems). The results of the CE were analyzed by Genemapper V3.5 software, through which the precise LsrR-binding sequence was obtained. As shown in Figure 4, one region from −20 to −49 bp is protected, which is indicated by the disappearing nucleotide peaks as the LsrR protein concentration increases (from line A to D). These footprinting results further demonstrated that LsrR binds to a 30-bp region (tga aca att gca tta aag att taa ata tgt) of its own promoter located just within the −35 to −10 promoter box relative to the translation start site (Figure 5A). By sequence alignment, we also found a 30-bp sequence (tca aaa ctc acc tgc aaa act gaa cgg ggg) from the lsrA promoter region, which shared 66.7% identity with the p-lsrR-box. To assess whether it is also the LsrR-binding site, we carried out further gel shift assays. Two 30-bp DNA fragments (p-lsrR-box and p-lsrA-box) were DIG-labeled and used as the probes. As expected, both of them are able to form complexes upon the addition of 5 to 40 pmol of LsrR in a 10 μl reaction mixture (Figure 5B).
Sequence analysis of LsrR-binding box and phenotypic assays of the mutated promoters of lsrR and lsrA
LsrR has been proposed to be a QS regulator that interacts with phospho-AI-2 and regulates transcription of a variety of genes in the previous studies 32, suggesting that LsrR might possess the ability to bind to diverse gene promoters. As mentioned above, the p-lsrR-box and p-lsrA-box do not share high similarity at the sequence level, indicating that the binding sites of LsrR are loosely conserved. To narrow down the sequences essential for LsrR binding, we performed a detailed analysis of the two binding boxes. By sequence alignment, we found that a 6-bp sequence (AAAACT in p-lsrA-box and AACAAT in p-lsrR-box) and a 9-bp sequence (AAAACTGAA in p-lsrA-box and AAGATTTAA in p-lsrR-box) are relatively conserved (Figure 6A), which led us to hypothesize that these two sequences might be crucial for LsrR binding. To test this, the β-galactosidase reporter plasmids pPlsrR and pPlsrA were used for assessing transcriptional activities of the lsrA and lsrR promoters 33. We also constructed a series of derivatives of the above plasmids by making corresponding deletions (Figure 6B). The above plasmids were transformed into E. coli DH5α (which is a luxS mutant strain, so that the transcriptional activities of lsrA and lsrR are repressed from the beginning to the end of the growth and cannot be activated by AI-2) and β-galactosidase activities were examined. The data in Figure 6C (a) show that, compared with pPlsrA, both pPlsrAD6 and pPlsrAD9 are associated with apparent increase in the expression of the LacZ reporter throughout the growth cycle. Correspondingly, as shown in Figure 6C (b), compared with pPlsrR, pPlsrRD6 and pPlsrRD9 are also associated with increase in the expression of the LacZ reporter. These results suggest that LsrR was unable to bind to the promoters of lsrA and lsrR firmly when the 6-bp or 9-bp sequences were deleted. Considering that deletion of 6 to 9 base-pair segments in such a binding site is likely to result in the reduction in binding affinity regardless of whether it included the exact binding motif or not, we constructed plasmids containing site-specific mutations (the original 6 to 9 base-pair AT-rich segments were replaced by random GCs) (Figure 6B), and conducted β-galactosidase activity assays after transformation of these mutant plasmids. As shown in Figure 6C, the transcriptional activities of p-lsrRM6, p-lsrRM9, p-lsrAM6, and p-lsrAM9 (similarly to that of p-lsrRD6, p-lsrRD9, p-lsrAD6, and p-lsrAD9) are all increased to higher levels compared with those of their parental promoters.
Characteristics of the LsrR protein
Three-dimensional structure prediction indicates that LsrR is composed of a typical HTH domain at the N-terminal and a sugar-ligand domain at the C-terminal. We found that when overexpressed in vitro, the C-terminal His6-tag LsrR protein mainly existed as tetramers rather than as monomers as shown by molecular sieve assays and the ratio was about 9:1 (data not shown). However, in the form of tetramer, LsrR still could bind to DNA probably because of the exposure of the N-terminal HTH domain. In vitro expressed His6-tag LsrR protein had the characteristic of strong binding affinity but weak stability, resulting in its quick denaturation and precipitation, thus bringing many challenges for the gel shift assays. Easy precipitation of the protein caused the DNA-protein complex to remain in the well and be unable to enter the gel. To solve the problem, many attempts including improvement of the protein purification methods and optimization of the experimental conditions were made. We found that the instability of the LsrR protein was mainly due to the instability of the N-terminal HTH domain. We had attempted to improve the stability of LsrR by adding the GST tag at its N-terminus, but it did make LsrR more stable and also dramatically decreased its DNA-binding affinity. It is likely that GST, due to its relatively large size (27 kD), affected the conformation of the LsrR N-terminal, which is responsible for the DNA binding. At last, through improving experimental conditions to overcome the problem of easy precipitation of the protein, we were able to use the C-terminal His6-tag LsrR protein to obtain the above results. The optimization included some details in protein preparation and gel shift assays. First, expression of LsrR should be induced at 16 °C; the purification should be performed at low temperature (4 °C) and high salt concentration (1 M), and imidazole in the eluant should be removed by ultrafiltration rather than dialysis. Second, during gel shift assays, the binding reaction and electrophoresis need to be carried out at room temperature and 4 °C, respectively; the samples should be loaded onto the well as quickly as possible, and the time of the electrophoresis should be within 1 h.
Bacteria have evolved complex mechanisms to regulate their physiological activities and behaviors in response to a variety of environmental cues. The AI-2-dependent QS system is considered to be the main QS system in E. coli and S. typhimurium, and many details about the mechanisms of this system still need to be further investigated. In S. typhimurium and E. coli, the AI-2 QS system regulates gene expression in a cytoplasmic AI-2-dependent manner. However, as the derivative of a furan ring, neither AI-2 nor phospho-AI-2 can directly interact with a target gene and function as a direct regulator to control gene transcription. The LsrR protein, a member of the transcription factor family based on the structural prediction, most probably acts as the important connector linking the detection of AI-2 to the regulation of gene expression 25, 28, 29, 32. The role of LsrR as a transcriptional regulator that responds to the QS signal AI-2 has been determined in the previous studies. Meanwhile, a greater impact of LsrR on gene expression control has been revealed. According to a previous study, 146 genes are significantly affected by the lsrR deletion. This includes genes related to secretion systems, biofilm formation, and genes coding for transcriptional regulators, small RNAs or riboregulators, and regulatory proteins for stress responses and nutrient acquisition 32. LsrR has even been hypothesized to be a global QS regulator that acts in tandem with unphosphorylated AI-2 or its anomer to regulate the transcription of a variety of genes. No matter whether this hypothesis is true or not, the regulatory mode of LsrR will be a topic of great interest. In this study, we performed experiments to study the binding characteristics of LsrR to the promoter regions and performed detailed analysis of the binding sequences, providing clues for the prediction of the target genes modulated by LsrR and illuminating the relationship between LsrR and the genes regulated by the AI-2 QS system.
A previous study has demonstrated that LsrR can bind to phospho-AI-2 in vitro, the effective form of the QS signal, indicating that LsrR is capable of signal detection 29. However, as a widely predicted transcription factor, LsrR has not been shown to directly bind to any DNA fragment so far. Previous physiological studies showed that LsrR represses the transcription of lsr operon and itself, but did not reveal whether the effects are through direct or indirect interactions between LsrR and these promoters. Our present study has provided direct evidence for the DNA-binding ability of LsrR, and confirmed that LsrR represses the transcription of lsr operon and itself by directly binding to their promoter regions.
The study of the LsrR-binding sequences in the promoter regions (p-lsrA and p-lsrR) shows that both on p-lsrA and p-lsrR, there exists a 30-bp binding box, which can be recognized by LsrR. The sequences are from −20 to −49 bp relative to the translation start codon of lsrR and from −5 to −34 bp relative to the translation start codon of lsrA. Interestingly, these regions represent the usual binding sites of transcription factors or the transcriptional machinery, such as σ factor and RNA polymerase. We propose that the effect of LsrR repression is due to its competitive inhibition of these transcriptional factors, thus making them unable to bind to the promoter regions. By comparing the two 30-bp binding boxes, we found that the similarity between the p-lsrR-box and the p-lsrA-box is not high at the nucleotide level (Figure 5A), indicating that the LsrR-binding sequences do not need to share high homology. However, we found two highly homologous motifs within the two binding boxes: AAAACTNN---NNAAAACTGAA in the p-lsrA-box and AACAATNN---NNAAGATTTAA in the p-lsrR-box, and the motifs contain high ratios of A and T. Results of the in vivo physiological experiments further revealed that either deletion or mutation of these motifs resulted in an apparent increase of the transcriptional activity of the promoters. We used E. coli DH5α, a LuxS/AI-2-negative strain as the host to test transcriptional activities of the promoters through measuring the β-galactosidase reporter activities. Our data showed that the transcriptional activities of the parental p-lsrR and p-lsrA remained at low levels throughout the growth cycle because of the LsrR repression; in contrast, the transcriptional activities of the p-lsrR and p-lsrA mutants with 6- to 9-bp deletions or random GC replacement increased to higher levels. These data indicate that the above motifs might be essential for LsrR binding. In addition, high ratios of A and T within these motifs suggest that LsrR might preferentially bind to AT-rich sequences.
Materials and Methods
Bacterial strains and media
The bacterial strains and plasmids used in this study are listed in Table 1. All strains were grown in Luria broth (LB), which contained 10 g/1 Bacto tryptone (Oxoid), 5 g/1 yeast extract (Oxoid), and 10 g/1 NaCl. When necessary, media were supplemented with antibiotics at the following concentrations (mg/1): ampicillin (Amp), 100; kanamycin (Kan), 50. All E. coli strains were grown at 37 °C with aeration.
To construct plasmids pGlsrR and pGlsrK used for the overproduction of LsrR and LsrK, the DNA fragment (954 bp) containing the lsrR coding region and the DNA fragment (1 593 bp) containing the lsrK coding region were amplified by polymerase chain reaction (PCR) from the chromosome of E. coli MG1655, respectively, using two pairs of primers (g-lsrR-f and g-lsrR-r; g-lsrK-f and g-lsrK-r). The PCR products were digested with NcoI and XhoI, and subsequently were ligated with NcoI and XhoI-digested pET28a(+) (Novagen). The inserts were checked by DNA sequencing. Plasmids pPlsrR and pPlsrA were the β-galactosidase reporter plasmids used for measuring the transcriptional activity of the lsrA and lsrR promoters 33. Plasmids pPlsrRD6, pPlsrRM6, pPlsrRD9, pPlsrRM9, pPlsrAD6, pPlsrRM6, pPlsrAD9, and pPlsrAD9 were the plasmids with mutated promoter regions (6- or 9-bp nucleotide bases deletion or mutation) of pPlsrR and pPlsrA (Figure 6B). To create them, the method of site-directed mutagenesis by PCR 34, 35 was used and modified. For example, consider pPlsrRD6: there were 6-bp nucleotide bases (AACAAT) in the lsrR promoter region, which were proposed to be necessary for LsrR binding, the 6-bp nucleotide bases were deleted from pPlsrA by the method as follows: a DNA fragment (about 10.5 kb, containing the full length of the plasmid except the 6-bp nucleotide bases) was amplified by PCR using the pPlsrR as the template, and D6-p-lsrR-f and D6-p-lsrR-r as the primers and Primer Star DNA polymerase (Takara). The PCR product was digested with DpnI (New England Biolabs), and then the digested product was phosphorylated, self-ligated, and transformed into E. coli DH5α. The positive clones with mutational promoter regions were checked by DNA sequencing. The sequences of all primers used in this study are listed in Table 2.
Purification of LsrR and LsrK
The same purification methods were used for LsrR and LsrK. Plasmid was transformed into E. coli BL21 (DE3). The transformant was grown in 1 L of LB at 37 °C to an OD600 of 0.4, transferred to 16 °C, and induced overnight with 0.1 mM IPTG. Cells were harvested by centrifugation and washed with cell washing buffer (20 mM Tris-HCl, pH 8.0 and 1 M NaCl). The cells were resuspended in 50 ml of lysis buffer (20 mM Tris-HCl, pH 8.0 and 1 M NaCl) and were then lysed by sonication, and centrifuged at 12 500 rpm for 30 min at 4 °C. The supernatant was mixed with 2 ml of Ni-NTA agarose solution (Invitrogen), and the suspension was loaded onto a column at 4 °C. After washing the column with 5 ml of washing buffer I (5 mM imidazole, 20 mM Tris-HCl, pH 8.0, and 1 M NaCl), and then with 100 ml of washing buffer II (20 mM imidazole, 20 mM Tris-HCl, pH 8.0, and 1 M NaCl) and 10 ml washing buffer III (100 mM imidazole, 20 mM Tris-HCl, pH 8.0, and 1 M NaCl), the LsrR protein was eluted with 5 ml of elution buffer (250 mM imidazole, 20 mM Tris-HCl, pH 8.0, 1 M NaCl, and 10% glycerol). The imidazole in the eluant was removed by using a Centrifuge Biomax-5 column (Millipore), and then the LsrR protein solution was stored at −80 °C until use. The purity of the protein was analyzed by SDS-PAGE and the protein concentration was measured using the Bradford assay with bovine serum albumin (BSA) as a standard.
Gel shift assay
Two DNA fragments p-lsrA (129 bp) containing lsrA promoter and p-lsrR (119 bp) containing lsrR promoter were prepared by PCR from chromosome of E. coli MG1655 using two pairs of primers (p-lsrA-f, p-lsrA-r, p-lsrR-f, and p-lsrR-r) and pfu DNA polymerase (Promega). The PCR products were purified by polyacrylamide gel electrophoresis (PAGE). The fragments p-lsrA-box (30 bp) and p-lsrR-box (30 bp) were synthesized by the manufacturer (Invitrogen). A digoxigenin (DIG) gel shift kit (second generation, Roche) was used for labeling of DNA fragments and detection of signals according to the manufacturer's instructions. Binding reactions were performed by incubating the labeled DNA fragments with various amounts of purified LsrR at 25 °C for 10-15 min in 10 μl of incubation buffer (50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 3 mM magnesium acetate, 0.1 mM EDTA, and 0.1 mM dithiothreitol (DTT)). After the incubation, the mixtures were added with 2.5 μl of gel loading buffer (0.25× TBE, 60%; glycerol, 40%; and bromphenol, 0.2% (wt/vol)), and then were electrophoresed in a 4% native polyacrylamide gel in 0.5× TBE buffer (45 mM Tris-borate and 1 mM EDTA; pH 8.0). DNA bands were detected and analyzed according to the manufacturer's instructions.
DNase I footprinting assay
PCR primer was synthesized and subsequently 5′-labeled with 6-FAM by the manufacturer (Invitrogen), resulting in the labeled primer p-lsrR-f-FAM. The labeled DNA fragments were prepared by PCR using E. coli MG1655 genomic DNA as template, labeled p-lsrR-f-FAM, and unlabeled p-lsrR-r as primers. The labeled DNA fragments were purified by PAGE. DNase I footprinting assays were performed with a 3730XL DNA analyzer (Applied Biosystems) using a modified method based on previous studies 36, 37. For DNA footprinting assay, the labeled promoter fragments (1 pmol) were incubated with purified LsrR in 50 μl of incubation buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 3 mM magnesium acetate, 0.1 mM EDTA, and 0.1 mM DTT) for 20 min at the room temperature; subsequently, the Ca2+/Mg2+ solution (50 μl, 5 mM CaCl2 and 10 mM MgCl2) was added into the mixture to a final volume of 100 μl and then were incubated for 5 min at 25 °C. DNA digestion was initiated by the addition of 0.6 U RQ1 RNase-Free DNase I (Promega). After digestion for 1 min, the digest reaction was terminated by adding with 90 μl stop solution (200 mM NaCl, 30 mM EDTA, and 1% SDS). The final reaction mixtures then were extracted with 200 μl phenol-chloroform-isoamyl alcohol (25:24:1) and purified by using the ethanol precipitation method. Samples were loaded onto a 3730XL DNA analyzer (Applied Biosystems) for CE and detection. Electropherograms were aligned using GeneMapper 3.5 (Applied Biosystems).
In vitro phosphorylation of AI-2
In vitro phosphorylation assays were performed with the methods as described previously 26, 29. The reactions were performed in 2 ml mixtures containing 1 mg of purified His-tagged LsrK, 25 mM Hepes, pH 7.5, 0.1 mg/ml BSA, and 1 mM DTT. Each sample contained 2 mM cold ATP, 100 mM MgCl2, and 1.3 mM AI-2 (DPD, Omm.Scientific Inc.). Reaction mixtures were incubated at 37 °C for 3 h, and then the phospho-AI-2 was size-fractionated by using a Centrifuge Biomax-5 column (Millipore) to remove the LsrK protein. Serial dilutions were applied to HPLC analysis (Agilent). In the phospho-AI-2 preparation, phosphorylated AI-2 is in a 1:1 molar ratio with ADP. Quantification of phosphorylated AI-2 was performed by measuring the concentration of ADP by HPLC (standard curve method). The conversion efficiency is the ratio of the quantity of phosphorylated AI-2 in the final preparation and that of AI-2 we utilized before the start of the reaction. Freshly prepared ATP and ADP stock solutions of known concentrations were used to draw the standard curves and confirm the accuracy of this method.
Stationary phase cultures of E. coli DH5α were diluted 1:500 into 20 ml of LB with 100 μg/ml ampicillin and grown for the specified times. After growth, cells were harvested and resuspended in 1 ml of Z buffer, and β-galactosidase assays were performed as described previously 38. β-Galactosidase units are defined as (OD420 × 1 000)/ (OD600 × Volume (ml) × Time (min)). All assays are reported as the mean β-galactosidase activity of three independent cultures, and error bars represent the standard deviations.
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We thank our colleagues J Zang and X Liu for their technical assistance in protein purification. This work was supported by the One Hundred Talent Project of the Chinese Academy of Sciences and the National Natural Science Foundation of China (50738006).
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