Abstract
P68 RNA helicase is a prototypical DEAD box RNA helicase. The protein plays a very important role in early organ development and maturation. Consistent with the function of the protein in transcriptional regulation and pre-mRNA splicing, p68 was found to predominately localize in the cell nucleus. However, recent experiments demonstrate a transient cytoplasmic localization of the protein. We report here that p68 shuttles between the nucleus and the cytoplasm. The nucleocytoplasmic shuttling of p68 is mediated by two nuclear localization signal and two nuclear exporting signal sequence elements. Our experiments reveal that p68 shuttles via a classical RanGTPase-dependent pathway.
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Introduction
In eukaryotic cells, the nucleus is separated from the cytoplasm. Maintenance of cellular functions requires trafficking of many bio-macromolecules into and out of the nucleus. Proteins that are targeted to the nucleus are marked by one or more sequence elements termed nuclear localization signal (NLS) 1, 2, while proteins that are transported out of the nucleus carry one or more nuclear exporting signal (NES) sequence tags 3. Nucleocytoplasmic trafficking occurs via the nuclear pore complex (NPC). Most protein transportation through the NPC is mediated by the interaction between specific cargos and a nuclear receptor system, importins and exportins 4, 5, 6. Interestingly, many proteins often carry both NLS and NES signals. This characteristic usually leads to shuttling of these proteins between the nucleus and the cytoplasm 7.
The nuclear p68 RNA helicase (hereafter referred to as p68) is a prototypical member of the DEAD box family of RNA helicases 8, 9. As an early example of a cellular RNA helicase, the ATPase and the RNA unwinding activities of p68 were previously documented 10, 11, 12. Expression of p68 correlates with cell proliferation and early organ maturation 13. P68 is suggested to function in DNA methylation/demethylation pathways 14. Our laboratory has demonstrated in vitro and in vivo that p68 is an essential splicing factor that plays a role in unwinding the transient U1:5′ splice site duplex 15, 16. Interestingly, results from several laboratories including our laboratory suggest that p68 may be involved in transcriptional regulation of a number of genes 17, 18, 19, 20, 21, 22, 23. Studies appear to suggest that p68 is involved in the transcriptional regulation by different mechanisms of action dependent on each individual regulated gene and cognate biological processes 13, 21, 24, 25, 26, 27. Experiments in our laboratory also demonstrate that p68 is phosphorylated at multiple amino acid residues, including serine/threonine and tyrosine 28, 29. Tyrosine phosphorylation of p68 correlates with tumor progression 25. Phosphorylation of p68 at Y593 mediates the effects of growth factors in promoting epithelial-mesenchymal-transition (EMT). The phospho-p68 promotes EMT by facilitating β-catenin nuclear translocation 30. In the present study, we demonstrate that p68 shuttles between the nucleus and the cytoplasm. P68 shuttling is mediated by two NLS and two NES sequence elements. Our data show that p68 shuttles via the classical RanGTPase-dependent pathway.
Results
P68 RNA helicase shuttles between the nucleus and the cytoplasm
We previously reported that Y593-phosphorylated p68 facilitates cytoplasmic β-catenin nuclear translocation by displacing the cytoplasmic β-catenin anchor protein axin 30. We reasoned that cytoplasmic localization of p68 is due to p68 shuttling between the nucleus and the cytoplasm. A number of nuclear localized proteins have been shown to be nucleocytoplasmic shuttles 31, 32. We thus employed a heterokaryon assay 33 using SW620 and NIH3T3 cells to test whether p68 shuttles between the nucleus and the cytoplasm. HA-tagged p68 proteins were exogenously expressed in SW620. After fusing the SW620 with NIH3T3 cells, the HA-p68 proteins were detected in the nucleus of NIH3T3 cells (Figure 1, upper panel). As a negative control, the non-shuttling protein MS2-DEK 34 expressed in SW620 cells could not be detected in the nucleus of NIH3T3 cells (Figure 1, bottom panel). These experimental results suggest that p68 is a nucleus-cytoplasm shuttling protein with a much longer residence time in the nucleus.
Identification of NLSs and NESs of p68
Most nucleocytoplasmic shuttling proteins carry sequence elements of both NLS and NES. We analyzed the amino acid sequence of p68 and found a number of sequence segments that resemble NLSs and NESs (Figure 2A and 3A). The NLS sequences were selected based on similarity to the classical SV40 and bipartite NLS sequences 35, 36, while the NES sequences were selected based on similarity to the consensus hydrophobic residue rich NES sequence, φX2-3φX2-3φXφ, where φ is a hydrophobic residue and X is any amino acid residue 37. To test the functionality of these putative NLSs and NESs in p68, we first fused each individual putative NLS or NES with a fluorescent protein DsRed. The fusion proteins were expressed in SW620 cells. It was clear that only NLS3 and NLS4 led to a substantial nuclear accumulation of the fluorescent protein (Figure 2B). To verify the functionality of NLS3 and NLS4, we made mutations in NLS3 (R352A, R353A, K360A and R362A) or NLS4 (R484A, R494A and K501A) in the context of full-length p68. The HA-tagged mutants were expressed in SW620 cells. Immunostaining of the exogenously expressed HA-p68, wt and the mutants, indicated that nuclear localization of the HA mutants was dramatically reduced (Figure 2C). Quantification of fluorescence intensity in the nucleus and the cytoplasm of a random group of cells confirmed the reduction of nuclear HA-p68 (the mean average of Cyto/Nu fluorescence intensity ratio were 0.038 ± 0.026 for wt, 1.477 ± 0.029 for NLS3-M and 1.489 ± 0.097 for NLS4-M). The results suggested that NLS3 and NLS4 indeed functioned as NLSs of p68. Fusion of NES2 and NES5 with the fluorescent protein resulted in high levels of cytoplasmic fluorescence protein (Figure 3B). Interestingly, fusion of NES8 with DsRed led to slightly higher levels of fluorescent protein localization in the cytoplasm (Figure 3B), indicating that NES8 is a weak nuclear export signal. Treatment of cells with leptomycin B (LMB) abolished the cytoplasmic localization patterns of DsRed fusion proteins (NES2, NES5 and NES8) (Figure 3C). The results suggested that NES2, NES5 and NES8 potentially function as nuclear export signals of p68. To further test the functions of NLSs and NESs of p68, we constructed several p68 deletion mutants (Figure 4A). These deletion mutants were expressed in SW620 cells either as HA-tagged proteins or as eGFP-fusion proteins (due to small sizes of truncates). Locations of each putative NLSs and NESs of p68 in the p68 truncation proteins are indicated. The localizations of these p68 truncates were analyzed by immunostaining with anti-HA antibody or imaging of eGFP. DII and DIII localized to the nucleus, indicating that NLS4 is a functional NLS, while the NES1 and NES7-8 are not functional NES. Strong cytoplasmic DIV and DV were observed, suggesting that one or more of NES2-6 are functional NES. The stronger fluorescence in DV than that of DIV and certain levels of cytoplasmic localization of DVI indicate a function of NLS3. In summary, these results support that NLS3 and NLS4 may function as NLSs of p68, while NES2 and NES5 may function as NESs of p68. Consistent with our experimental results, NLS3 is located in an exposed helical secondary structure, while NLS4 is located in an exposed loop that is flanked by two α-helixes in a computer-simulated p68 model structure (Figure 4B). Both NES5 and NES8 are also located on the exposed surface in the model structure, while NES2 is buried under an α-helix in the model structure. This structure model provided additional support for the identification of the NLSs and NESs of p68.
We next mutated both NLS3 and NLS4 by the same mutations described above (hereafter referred to as NLS-M). The mutant was expressed in SW620, T98G and SW480 cells. Immunofluorescence staining demonstrated that no significant levels of p68 mutant localized in the cell nucleus (Figure 2D). The results were further verified by immunoblot analyses of exogenously expressed HA-p68s (wt and the mutant) in the cytoplasmic and nuclear extracts made from HA-p68s-expressing T98G cells (Figure 2E). It was also clear that the mutant no longer shuttled between the nucleus and the cytoplasm as demonstrated by the heterokaryon assay (Figure 1, 2nd panel from top). The distribution pattern did not change significantly when the heterokaron cells were treated with LMB (data not shown). In our structure model, NES5 and NES8 are well exposed, while NES2 is buried. Thus, we constructed the p68 NES mutant by mutations at both NES5 and NES8, with charges of F293A, L294A, L298A, L305A, L456A and I457A (hereafter referred to as NES-M). Immunofluorescence staining of SW620 cells that expressed the mutant showed an exclusive nuclear localization of the mutant (Figure 3D). Heterokaryon assay demonstrated that the nucleocytoplasmic shuttling of p68 was almost completely abolished by the mutations (Figure 1, 3rd panel from top). In contrast, mutations separately on either NES5 or NES8 (same mutations) did not completely abolish but certainly reduced the shuttling of HA-p68 to the NIH3T3 cell nucleus (Figure 1, 4th and 5th panels from top). Our mutational analyses confirmed that the NLS3/NLS4 and NES5/NES8 elements were functional NLSs and NESs, and that these NLSs and NESs were required for p68 nucleocytoplasmic shuttling.
P68 shuttles via a RanGTPase-dependent pathway
CRM1 is an export receptor mediating nuclear export of proteins that carry leucine-rich NESs 38, 39. We demonstrated that p68 RNA helicase carries NLS and NES sequences that mediate nucleocytoplasmic shuttling. We thus asked whether overexpression of CRM1 would affect the localization of p68. We exogenously expressed CRM1 in SW620 cells (Figure 5A). Immunoblot of p68 indicated that there were significantly higher levels of cytoplasmic p68 after CRM1 was exogenously expressed in the cells, and the increases in cytoplasmic p68 were inhibited by LMB treatment (Figure 5B). Increases in cytoplasmic p68 by expression of CRM1 were not observed with p68 mutant that carries mutations at both NES5 and NES8 (Figure 5C). To further confirm the effects of CRM1 on export of p68, we probed the interaction between p68 and endogenous CRM1 via co-immunoprecipitation. It was clear that p68 co-immunoprecipitated with endogenous CRM1 (Figure 5D). The results suggest that p68 RNA helicase was exported from the nucleus mediated by the exportin pathway. The effects of exogenous expression of CRM1 on p68 export were further confirmed by immunostaining analyses. It was evident that staining of p68 in the cytoplasm was significantly increased upon the expression of CRM1, and the increases were inhibited by LMB (Figure 5E). To further verify that the p68 nucleocytoplasmic shuttling is mediated by the RanGTPase pathway, we tested whether p68 interacts with importin in vitro. We used a commercially available his-tag importin α2. When his-importin α2 was incubated with recombinant GST-p68, GST-p68 could not be pulled down by his-importin α2 (Figure 6A). It is known that importin α2 functions as a hetero-dimer with importin β 40, 41. Therefore, we added another commercially available recombinant importin β1. It was clear that GST-p68 co-precipitated with his-importin α2 when importin β1 was also present, but the p68 NLS mutant (NLS-M) did not co-precipitate with his-importin α2 under any condition (Figure 6A). We also carried out co-precipitation experiments with immunopurified HA-p68 from HEK293 cells. The his-importin α2/importin β1 co-immunoprecipitated with the purified HA-p68 using anti-HA antibody. However, the importins did not co-immunoprecipitate with HA-p68 NLS mutant (Figure 6B). The interaction of p68 with importins was also verified by co-immunoprecipitation experiment with endogenous importin α2/β1 (Figure 6C). The observations suggest that p68 interacts with the importin α2/β1 dimer, providing additional support for the nucleocytoplasmic shuttling of p68 via the RanGTPase pathway.
Discussion
P68 RNA helicase was shown to predominately localize in the cell nucleus 42. However, recent experiments carried out in Janknecht's and our laboratories showed a transient cytoplasmic localization of the protein 19, (Gao and Liu, unpublished observations). In this report, we presented data demonstrating the nucleocytoplasmic shuttling of p68 RNA helicase. Our experiments showed that p68 shuttling is mediated by two NLS and two NES sequence elements. The p68 nuclear export and import follow a RanGTPase-dependent pathway. Interestingly, a number of DEAD/DExH box RNA helicases have also been shown to shuttle between the nucleus and cytoplasm, including eIF-4AIII, An3, GRTH/Ddx25 and RNA helicase A 34, 43, 44, 45. P68 RNA helicase was previously shown to interact with mRNA/mRNP 16. Thus, one possibility is that the p68 export and shuttling is associated with mRNP export. It was shown that shuttling of GRTH/Ddx25 is dependent upon mRNP exporting 43. However, the interaction of p68 with CRM1 is RNA-independent, as p68 still co-immunoprecipitated with CRM1 in the nuclear extracts treated by RNase (data not shown). Although the result did not prove that p68 nuclear export is mRNA export-independent, the result does suggest a possibility that mRNA exporting is not necessarily required for the p68 nuclear exporting.
The cellular function(s) of the p68 RNA helicase nucleocytoplasmic shuttling is an open question. In general, proteins that shuttle between the nucleus and the cytoplasm usually have distinct cellular functions in these two separated subcellular compartments. They often re-localize to the nucleus or the cytoplasm to fulfill specific functional role(s) in response to particular cellular signal stimuli 46, 47. Alternatively, nucleocytoplasmic shuttling of a protein can function as 'chaperons' to help nuclear import or export of other proteins or RNAs 48, 49. Cellular functions of p68 helicase in the cell nucleus are well documented. The protein is functionally involved in gene transcription 21, 22, 23, 50, pre-mRNA 15, 16, pre-rRNA and pre-miRNA processing 51, 52. Whether p68 RNA helicase has any potential function(s) in the cytoplasm is currently not very clear. Goh et al. 53 detected the interaction of p68 with HCV-NS5B in the cytoplasm in the viral-infected or NS5B-expressing 293 cells and the interaction is essential for viral replication. In another independent study, Harris et al. 54 found that the 3′-nontranslated region of HCV interacts with p68 in an RNA affinity capture experiment using the cytoplasmic extracts of 293 cells that express the viral replicon. These observations are consistent with our experimental results indicating that p68 localizes both in the nucleus and the cytoplasm. However, despite the demonstration of the function of p68 in HCV replication in the cytoplasm of virus-infected cells, evidence suggesting the involvement of p68 in any cellular process in the cytoplasm is currently lacking. We have observed a significant increases in cytoplasmic p68 levels upon treatment of cells with several growth factors and chemokines 30, (Wang, unpublished observations), indicating that p68 may function in the cytoplasm under specific cellular conditions, such as abnormal growth or cell migration in response to growth factor or chemokine stimulations.
Localization of p68 is intriguing. The protein is shuttling between the nucleus and the cytoplasm, while the protein predominantly resides in the cell nucleus, and is nearly undetectable outside of the nucleus by immunostaining or by immunoblot analyses of cytoplasmic extracts. The phenomenon suggests that the function of the NESs of p68 is tightly regulated. It is possible that an interacting partner(s) in the nucleus masks the p68 NES sequences. Nuclear export of p68 may thus depend on the dissociation of the interaction partner(s). Our data demonstrate that p68 has three sequence elements that potentially function as NESs. On the other hand, structural modeling of p68 helicase core showed that one of the potential NES (NES2) is buried under an α-helix. Therefore, it is possible that a specific post-translational modification drives conformational changes, which subsequently expose this buried NES sequence for p68 export 55. Very similar regulatory mechanisms were observed in nuclear exporting and importing of Dok1 and NF-AT1 56, 57. Consistent with this notion, we observed a substantially increased cytoplasmic p68 level when the cells were treated with several growth factors and chemokines 30, (data not shown), suggesting a possibility that the treatments of growth factors and chemokines may trigger the exposure of the NES sequences for nuclear export of p68. There are two NLS sequences. Mutation of either NLS reduced nuclear localization (increased cytoplasmic p68, Figure 2C), indicating that both NLSs contribute partly to the nuclear localization of p68.
Materials and Methods
Reagents, antibodies and cells
LMB, PEG3350, cycloheximide, HA peptide and protease inhibitor cocktail were purchased from Calbiochem, Sigma and Roche. Antibodies against HA and His tags, GAPDH, Lamin A/C, β-actin, and Histone 2A were purchased from Roche, Cell signaling, Upstate and Chemicon. The monoclonal antibody P68-RGG and polyclonal antibody Pabp68 against p68 were produced in our laboratory. Cell lines SW480, SW620, HT29, NIH3T3 and T98G were purchased from ATCC and cultured by following the vendor's instructions.
Plasmids construction
The HA-tagged p68s expression plasmids (wild-type, and Y593F, Y595F, LGLD and NLS-M mutants) were constructed in pHM6 vectors as previously reported 30. The vectors for expression of eGFP and DsRed fusion proteins were constructed using eGFP-pcDNA-3.1(+) or pDsRed1-N1 vectors. Full-length p68 or deletion mutants of p68 variants were subcloned into the vector by EcoR321 and NotI restriction sites. The p68 or deletion mutants were fused at the C-terminal of the fluorescent protein tag. Different putative NLS- and NES-tagged DsRed were constructed by cloning into pDsRed-N1 vectors at 5′ (BamHI and EcoRI sites) with the addition of a starting methionine at the N-terminal of each NLS and NES, respectively. Site-directed mutagenesis was performed using QuikChange® Multi Site-Directed Mutagenesis Kit (Stratagene). All the DNA clones and mutations were verified by auto-DNA sequencing at GSU. The procedures for transfection of expression vectors of HA-p68, fluorescent proteins, and Crm1 and duplex RNAi were similar to our previous reports 30, 50.
Expression and purification of recombinant GST-p68
Recombinant GST-p68 was expressed and purified as described in our previous report 58.
Interactions between p68 and importins
Recombinant GST-p68 (8 μg) was incubated with commercial his-importin α2 (8 μg) and/or importin β1 (Calbiochem) overnight at 4 °C in 500 μl in PBS buffer. After incubation, the protein complex was pulled down by Ni-TED silica beads. The pull-down proteins were separated by 10% SDS-PAGE, and were analyzed by immunoblot using anti-GST or anti-his-tag antibodies. The presence of importin β1 was visualized by ponceau S staining. For co-immunoprecipitation of HA-p68 with importin α2, HA-p68s, wt or mutant, were expressed in HEK cells and were immunoprecipated using 30 βl anti-HA antibody. Protein G agarose (50 μl) was added to the mixture. After extensive washing, HA-p68s were eluted from the beads by competition using HA peptide (100 μg/ml). The eluted HA-p68s were dialyzed against PBS. The recombinant importin α2 and/or β1 was incubated with the purified HA-p68 in PBS for 4 h. The protein mixtures were immunoprecipated using anti-HA antibody. The precipitated complex was separated in 10% SDS-PAGE followed by immunoblot using anti-his-tag antibody. The presence of HA-p68 was detected by immunoblot using the antibody p68-rgg.
Heterokaryon analyses and immunofluoresence imaging
The experimental procedures for immunofluoresence staining and imaging were similar to our previous reports 30, 59. For heterokaryon analyses, SW620 cells were first transfected with HA-p68s (wt or mutants) or MS2-DEK. The cells were mixed with an equal number of mouse NIH3T3 cells 24 h post-transfection and reseeded in four-well chambers. Subsequently, 50 μg/ml of cycloheximide was added to the culture medium to inhibit protein synthesis. After 3 h, the co-cultured cells were fused using 50% PEG3350 for 2 min, washed and incubated with the medium containing 75 μg/ml cycloheximide for 3 h. The treated cells were then fixed and immunostained as described above. Rabbit polyclonal anti-MS2 antibody was employed to stain MS2-DEK, followed by Alex Fluor 555 goat anti-rabbit IgG antibody. In a random group of 30 cells, the numbers of cells with both NIH3T3 and SW620 nucleus and with HA-p68 in NIH3T3 nucleus or in the cytoplasm of fused cells were counted. The percentage of HA-p68 in the nucleus or cytoplasm of fused cells equals fused cells with HA-p68 in NIH3T3 nucleus or cytoplasm/total fused cells with expression of HA-p68.
Computational homology structure modeling the helicase core of p68
The sequence alignment of p68 RNA helicase core with homologous RNA helicase cores of several DEAD box family proteins (e.g., vasa, eIF4A, Dhhlp and UAP56) was performed by the program ClustalW. The secondary structure elements were predicted based on the consensus analysis using computational programs JPRED, PHD and PSIPRED 60, 61. The homology modeling of the p68 RNA helicase structure was constructed using the homology-modeling server SWISS-MODEL based on X-ray crystal structure of Drosophila vasa 62, which has the highest alignment score with p68 RNA helicase core. The putative NLSs and NESs were indicated in the modeling structure.
References
Goldfarb DS, Gariepy J, Schoolnik G, et al. Synthetic peptides as nuclear localization signals. Nature 1986; 322:641–644.
Schneider J, Schindewolf C, van Zee K, et al. A mutant SV40 large T antigen interferes with nuclear localization of a heterologous protein. Cell 1988; 54:117–125.
Wen W, Meinkoth, JL, Tsien, RY, et al. Identification of a signal for rapid export of proteins from the nucleus. Cell 1995; 82:463–473.
Nakielny S, Dreyfuss G . Transport of proteins and RNAs in and out of the nucleus. Cell 1999; 99:677–690.
Gorlich D, Kutay U . Transport between the cell nucleus and the cytoplasm. Annu Rev Cell Dev Biol 1999; 15:607–660.
Hill CS . Nucleocytoplasmic shuttling of Smad proteins. Cell Res 2009; 19:36–46.
Lusk CP, Blobel G, King MC . Highway to the inner nuclear membrane: rules for the road. Nat Rev Mol Cell Biol 2007; 8:414–420.
Crawford L, Leppard K, Lane D, et al. Cellular proteins reactive with monoclonal antibodies directed against simian virus 40 T-antigen. J Virol 1982; 42:612–620.
Lane DP, Hoeffler WK . SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000. Nature 1980; 288:167–170.
Iggo RD, Lane DP . Nuclear protein p68 is an RNA-dependent ATPase. EMBO J 1989; 8:1827–1831.
Ford MJ, Anton IA, Lane DP . Nuclear protein with sequence homology to translation initiation factor eIF-4A. Nature 1988; 332:736–738.
Hirling H, Scheffner M, Restle T, et al. RNA helicase activity associated with the human p68 protein. Nature 1989; 339:562–564.
Stevenson RJ, Hamilton SJ, MacCallum DE, et al. Expression of the 'dead box' RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetus. J Pathol 1998; 184:351–359.
Jost JP, Schwarz S, Hess D, et al. A chicken embryo protein related to the mammalian DEAD box protein p68 is tightly associated with the highly purified protein-RNA complex of 5- MeC-DNA glycosylase. Nucleic Acids Res 1999; 27: 3245–3252.
Liu ZR . p68 RNA helicase is an essential human splicing factor that acts at the U1 snRNA-5′ splice site duplex. Mol Cell Biol 2002; 22:5443–5450.
Lin C, Yang L, Yang JJ, et al. ATPase/helicase activities of p68 RNA helicase are required for pre-mRNA splicing but not for assembly of the spliceosome. Mol Cell Biol 2005; 25:7484–7493.
Fujita T, Kobayashi Y, Wada O, et al. Full activation of estrogen receptor alpha activation function-1 induces proliferation of breast cancer cells. J Biol Chem 2003; 278:26704–26714.
Watanabe M, Yanagisawa J, Kitagawa H, et al. A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA. EMBO J 2001; 20:1341–1352.
Rossow KL, Janknecht R . Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300. Oncogene 2003; 22:151–156.
Wilson BJ, Bates GJ, Nicol SM, et al. The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner. BMC Mol Biol 2004; 5:11.
Endoh H, Maruyama K, Masuhiro Y, et al. Purification and identification of p68 RNA helicase acting as a transcriptional coactivator specific for the activation function 1 of human estrogen receptor alpha. Mol Cell Biol 1999; 19:5363–5372.
Bates GJ, Nicol SM, Wilson BJ, et al. The DEAD box protein p68: a novel transcriptional coactivator of the p53 tumour suppressor. EMBO J 2005; 24:543–553.
Buszczak M, Spradling AC . The Drosophila P68 RNA helicase regulates transcriptional deactivation by promoting RNA release from chromatin. Genes Dev 2006; 20:977–989.
Kahlina K, Goren I, Pfeilschifter J, et al. p68 dead box RNA helicase expression in keratinocytes: regulation, nucleolar localization, and functional connection to proliferation and VEGF gene expression. J Biol Chem 2004; 279:44872–44882.
Yang L, Lin C, Liu ZR . Phosphorylations of DEAD box p68 RNA helicase are associated with cancer development and cell proliferation. Mol Cancer Res 2005; 3:355–363.
Wei Y, Hu MH . [The study of P68 RNA helicase on cell transformation]. Yi Chuan Xue Bao 2001; 28:991–996.
Warner DR, Bhattacherjee V, Yin X, et al. Functional interaction between Smad, CREB binding protein, and p68 RNA helicase. Biochem Biophys Res Commun 2004; 324:70–76.
Yang L, Liu ZR . Bacterially expressed recombinant p68 RNA helicase is phosphorylated on serine, threonine, and tyrosine residues. Protein Expr Purif 2004; 35:327–333.
Yang L, Lin C, Liu ZR . Signaling to the DEAD box-Regulation of DEAD-box p68 RNA helicase by protein phosphorylations. Cell Signal 2005; 17:1495–1504.
Yang L, Lin C, Liu ZR . P68 RNA helicase mediates PDGF-induced epithelial mesenchymal transition by displacing axin from beta-catenin. Cell 2006; 127:139–155.
Cartwright P, Helin K . Nucleocytoplasmic shuttling of transcription factors. Cell Mol Life Sci 2000; 57:1193–206.
Zhu J and McKeon F . Nucleocytoplasmic shuttling and the control of NF-AT signaling. Cell Mol Life Sci 2000; 57:411–420.
Fan XC, Steitz JA . Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs. EMBO J 1998; 17:3448–3460.
Shibuya T, Tange TO, Sonenberg N, et al. eIF4AIII binds spliced mRNA in the exon junction complex and is essential for nonsense-mediated decay. Nat Struct Mol Biol 2004; 11:346–351.
Kalderon D, Roberts BL, Richardson WD, et al. A short amino acid sequence able to specify nuclear location. Cell 1984; 39 (Part 2):499–509.
Robbins J, Dilworth SM, Laskey RA, et al. Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 1991; 64:615–623.
Neumann G, Hughes MT, Kawaoka Y . Influenza A virus NS2 protein mediates vRNP nuclear export through NES-independent interaction with hCRM1. EMBO J 2000; 19:6751–6758.
Fornerod M, Ohno M, Yoshida M, et al. CRM1 is an export receptor for leucine-rich nuclear export signals. Cell 1997; 90:1051–1060.
Watanabe M, Fukuda M, Yoshida M, et al. Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells and fission yeast. Genes Cells 1999; 4:291–297.
Goldfarb DS, Corbett AH, Mason DA, et al. Importin alpha: a multipurpose nuclear-transport receptor. Trends Cell Biol 2004; 14:505–514.
Lindsay ME, Plafker K, Smith AE, et al. Npap60/Nup50 is a tri-stable switch that stimulates importin-alpha:beta-mediated nuclear protein import. Cell 2002; 110:349–360.
Nicol SM, Causevic M, Prescott AR, et al. The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase. Exp Cell Res 2000; 257:272–280.
Sheng Y, Tsai-Morris CH, Gutti R, et al. Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis. J Biol Chem 2006; 281:35048–35056.
Aratani S, Oishi T, Fujita H, et al. The nuclear import of RNA helicase A is mediated by importin-alpha3. Biochem Biophys Res Commun 2006; 340:125–133.
Askjaer P, Bachi A, Wilm M, et al. RanGTP-regulated interactions of CRM1 with nucleoporins and a shuttling DEAD-box helicase. Mol Cell Biol 1999; 19:6276–6285.
Xu L, Massague J . Nucleocytoplasmic shuttling of signal transducers. Nat Rev Mol Cell Biol 2004; 5:209–219.
Kau TR, Way JC, Silver PA . Nuclear transport and cancer: from mechanism to intervention. Nat Rev Cancer 2004; 4:106–117.
Loyola A, Almouzni G . Histone chaperones, a supporting role in the limelight. Biochim Biophys Acta 2004; 1677:3–11.
Fried H, Kutay U . Nucleocytoplasmic transport: taking an inventory. Cell Mol Life Sci 2003; 60:1659–1688.
Yang L, Lin C, Zhao S, et al. Phosphorylation of p68 RNA helicase plays a role in platelet-derived growth factor-induced cell proliferation by up-regulating cyclin D1 and c-Myc expression. J Biol Chem 2007; 282:16811–16819.
Davis BN, Hilyard AC, Lagna G, et al. SMAD proteins control DROSHA-mediated microRNA maturation. Nature 2008; 454:56–61.
Fukuda T, Yamagata K, Fujiyama S, et al. DEAD-box RNA helicase subunits of the Drosha complex are required for processing of rRNA and a subset of microRNAs. Nat Cell Biol 2007; 9:604–611.
Goh PY, Tan YJ, Lim SP, et al. Cellular RNA helicase p68 relocalization and interaction with the hepatitis C virus (HCV) NS5B protein and the potential role of p68 in HCV RNA replication. J Virol 2004; 78:5288–5298.
Harris D, Zhang Z, Chaubey B, et al. Identification of cellular factors associated with the 3'-nontranslated region of the hepatitis C virus genome. Mol Cell Proteomics 2006; 5:1006–1018.
Wrighton KH, Lin X, Feng XH . Phospho-control of TGF-beta superfamily signaling. Cell Res 2009; 19:8–20.
Niu Y, Roy F, Saltel F, et al. A nuclear export signal and phosphorylation regulate Dok1 subcellular localization and functions. Mol Cell Biol 2006; 26:4288–4301.
Okamura H, Aramburu J, Garcia-Rodriguez C, et al. Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity. Mol Cell 2000; 6:539–550.
Huang Y, Liu ZR . The ATPase, RNA unwinding, and RNA binding activities of recombinant p68 RNA helicase. J Biol Chem 2002; 277:12810–12815.
Wang H, Liu Y, Gao X, et al. The recombinant beta subunit of C-phycocyanin inhibits cell proliferation and induces apoptosis. Cancer Lett 2007; 247:150–158.
Cuff JA, Clamp ME, Siddiqui AS, et al. JPred: a consensus secondary structure prediction server. Bioinformatics 1998; 14:892–893.
McGuffin LJ, Bryson K, Jones DT . The PSIPRED protein structure prediction server. Bioinformatics 2000; 16:404–405.
Sengoku T, Nureki O, Nakamura A, et al. Structural basis for RNA unwinding by the DEAD-box protein Drosophila Vasa. Cell 2006; 125:287–300.
Acknowledgements
We thank Drs Joan A Steitz (Yale University School of Medicine), Melissa J Moore (University of Massachusetts medical school) and Hung-Ying Kao (Case Western Reserve University) for providing the vectors for expression of MS2-DEK and human CRM1. We are grateful to Professor Peter Stockley (University of Leeds) for providing antibody against MS2-DEK. We also thank Birgit Neuhaus (Georgia State University) for assistance in confocal imaging. This manuscript is greatly improved by comments from Christie Carter, Michael Kirberger and Heena Dey (Georgia State University). This work is supported in part by research grants from National Institutes of Health (GM063874 and CA118113) and Georgia Cancer Coalition to ZR Liu. X Gao is supported by an MBD fellowship, GSU.
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Wang, H., Gao, X., Huang, Y. et al. P68 RNA helicase is a nucleocytoplasmic shuttling protein. Cell Res 19, 1388–1400 (2009). https://doi.org/10.1038/cr.2009.113
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DOI: https://doi.org/10.1038/cr.2009.113
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