Abstract
Brassinosteroids (BRs) are a major group of plant hormones that regulate plant growth and development. BRI1, a protein localized to the plasma membrane, functions as a BR receptor and it has been proposed that its kinase activity has an essential role in BR-regulated plant growth and development. Here we report the isolation and molecular characterization of a new allele of bri1, bri1–301, which shows moderate morphological phenotypes and a reduced response to BRs under normal growth conditions. Sequence analysis identified a two-base alteration from GG to AT, resulting in a conversion of 989G to 989I in the BRI1 kinase domain. An in vitro assay of kinase activity showed that bri1-301 has no detectable autophosphorylation activity or phosphorylation activity towards the BRI1 substrates TTL and BAK1. Furthermore, our results suggest that bri1-301, even with extremely impaired kinase activity, still retains partial function in regulating plant growth and development, which raises the question of whether BRI1 kinase activity is essential for BR-mediated growth and development in higher plants.
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Introduction
Brassinosteroids (BRs) are natural plant growth-promoting compounds with a structure similar to that of animal steroid hormones. Recent progress in plant molecular genetics has shown that, although BRs are distributed in plants at an extremely low level, they have significant effects on plant growth and development, including seed germination, stem and root elongation, seedling photomorphogenesis, vascular differentiation, leaf development, pollen tube growth, stress resistance, and senescence 1, 2.
Unlike animal steroid hormones that bind to nuclear receptors and directly activate target genes 3, BRs are perceived at the plasma membrane by BRI1, a leucine-rich repeat receptor-like kinase (LRR-RLK) in Arabidopsis thaliana 4, 5, 6, 7. Homologs of BRI1 have also been identified in rice, tomato, barley, pea, and cotton 8, 9, 10, 11, 12. The BRI1 contains a leucine-rich repeat (LRR) extracellular domain, a transmembrane domain, and a cytoplasmic kinase domain with serine/threonine specificity 6, 13. Studies have shown that the extracellular domain, especially the island and the neighboring C-terminal LRR repeat, is responsible for perceiving BRs 5. It has been proposed that the kinase activity of BRI1 is essential for the biological functions that mediate plant growth and development 13.
BAK1, another LRR-RLK that is localized to the plasma membrane, was identified by searching for BRI1-interacting proteins using a yeast two-hybrid screen and by screening for bri1 suppressors with an activation-tagging approach in A. thaliana 14, 15. It has been shown that the interaction of BAK1 with BRI1 forms a heterodimer, which initiates the signal transduction cascade 16. BAK1 is phosphorylated as a result of binding to BRI1 14, 15, 17. Furthermore, another two BRI1-interacting proteins, transthyretin-like (TTL) and BRI1 kinase inhibitor 1 (BKI1), have been identified by a yeast two-hybrid screen using the BRI1 kinase domain as bait 18, 19. TTL and BKI1 are substrates of BRI1 and act as negative regulators in BR-mediated plant growth 18, 19.
In this paper, we report the isolation and characterization of a new bri1 allele, bri1-301, which shows a weak morphological phenotype and a reduced sensitivity to BRs. The mutation from GG to AT in bri1-301 results in the conversion of 989G to 989I in the BRI1 kinase domain, which leads to undetectable kinase activity in bri1-301. Our findings from this new bri1 allele raise questions about the role of BRI1 kinase activity in plant growth and development.
Results
Isolation and characterization of a new bri1 allele, bri1-301
By using a two-step screening approach (see Materials and Methods), we isolated a morphological mutant with a much shorter hypocotyl compared with the wild type when grown under long-wavelength light (> 460 nm) (Figure 1A). In addition, when grown under white light conditions, the mutant displayed weak morphological abnormalities, including round leaves, short petioles, prolonged life span, and slightly shorter plant height with nearly normal fertility (Figures 1B and 1C). However, the mutant exhibited hypocotyl elongation under white light conditions and etiolated phenotype in the dark, similar to the wild type (Figure 1A).
To understand the molecular basis of this mutant, we cloned the gene responsible for the mutant phenotype using a map-based approach. Briefly, genetic analysis showed that all the 41 F1 progeny from a cross between the mutant and a wild-type plant had the wild-type phenotype. In the F2 generation there were 858 wild-type to 289 mutant plants. These results indicate that the mutant possesses a recessive mutation at a single nuclear locus. Using an F2 mapping population derived from a cross between the mutant and its polymorphic ecotype Landsberg erecta (Ler), the mutant gene was found to be tightly linked to the BRI1 locus 6 (data not shown). An allelic test in which the mutant was crossed with bri1-101, a strong allele of bri1 13, showed that the mutant is allelic to bri1 (data not shown). Therefore, the mutant was designated bri1-301, which has been extensively used in the study of BR signaling pathways 14, 18, 20, 21, 22.
Molecular lesion in bri1-301
The molecular lesion in bri1-301 was identified by comparing the genomic DNA sequences of the mutant and wild-type plants. A two-base alteration from GG to AT, which causes a conversion from Gly to Ile of codon 989 in the VIa subdomain of the kinase domain of BRI1, was identified (Figure 2). This two-base change in bri1-301 resulted in the elimination of the MboI restriction enzyme site, which allowed us to develop a co-dominant cleaved amplified polymorphic sequences (CAPS) marker to distinguish between bri1-301 and wild-type plants (Figure 2).
Altered expression patterns of light-regulated genes in bri1-301
The bri1-301 mutant was isolated using a two-step screening approach and each step was under a different light condition, suggesting that BRI1 may be involved in the light signaling pathway. To find out whether the altered response of bri1-301 to light resulted from an interruption to the light signaling pathway, we examined the expression patterns of two light-inducible genes, RbcS1A and Lhcb1.3, using real-time PCR analysis 23, 24, 25. As shown in Figure 3, the expression levels of RbcS1A and Lhcb1.3 in the wild-type seedlings are greatest under white light, but are reduced to approximately 50% under long-wavelength light and to 20% in the dark. In bri1-301, the expression levels of RbcS1A and Lhcb1.3 under long-wavelength light are almost the same as the levels under white light. Although the expression of RbcS1A and Lhcb1.3 in bri1-301 is reduced in the dark, the expression levels are >40% of the expression levels under white light. The expression patterns of RbcS1A and Lhcb1.3 in bri1-101 are similar to those of bri1-301 (Figure 3). These results demonstrate that the mutation in BRI1 was able to enhance the expression of the light-inducible genes RbcS1A and Lhcb1.3, both under long-wavelength light and in the dark, which suggests that BRI1 participates in the light signaling pathway.
Response of bri1-301 to BRs
As an essential plant growth regulator, BRs can affect plant morphology at an extremely low concentration. As shown in Figure 4, the wild-type seedlings responded to exogenous 24-epi-brassinolide (24-epi-BL) in a concentration-dependent manner (Figure 4A). The strong allele bri1-101 seedlings showed nearly undetectable additional phenotypic alterations at either low or high concentrations of BL (Figure 4B), whereas the bri1-301 seedlings responded to exogenous BL at a high concentration (1 μM) with a stimulated effect (Figure 4C). These results suggest that the response of bri1-301 to BRs is altered.
To substantiate the altered response of bri1-301 to exogenous BL, we compared the expression levels of BAS1 and CPD among wild-type, bri1-101, and bri1-301 plants. BAS1 encodes a cytochrome P450 that is involved in BL degradation and is upregulated by BRs 26. CPD encodes a steroid hydroxylase that is involved in BR biosynthesis and is downregulated by BRs 27. In wild-type plants, the expression of BAS1 was significantly induced by exogenous BL, but its expression was only slightly affected by BL in both bri1-301 and bri1-101 (Figure 4D). In wild-type plants, expression of CPD was strongly suppressed by BL (Figure 4D). However, in bri1-301, CPD expression was only moderately affected compared with the wild type (Figure 4D).
Kinase activity is undetectable in bri1-301
To understand whether the Gly-to-Ile missense replacement in the kinase domain of bri1-301 affects its kinase activity, we expressed and purified the bri1-301 kinase domain (bri1-301-KD), fused with glutathione S-transferase (GST), and assayed its kinase activity in vitro (Figure 5). The expressed kinase domain of the wild type, BRI1-KD, showed strong autophosphorylation activity as previously reported 13, whereas the kinase activity of the expressed bri1-301-KD or bri1-101-KD was undetectable (Figure 5A). In addition, we noticed that the electrophoretic mobility of BRI1-KD was slower than that of both bri1-301-KD and bri1-101-KD (Figure 5A), which suggests that the slower mobility may result from the autophosphorylation of BRI1-KD. In fact, after treatment with calf intestinal phosphatase (CIP), BRI1-KD showed the same electrophoretic mobility as bri1-301-KD and bri1-101-KD, which were not affected by the CIP treatment (Figure 5B). These results demonstrate that both bri1-301-KD and bri1-101-KD have defective autophosphorylation.
To test whether bri1-301 or bri1-101 has also lost its ability to phosphorylate its native substrates (an example is TTL 18), we expressed and purified the full-length TTL protein, fused with maltose binding protein (MBP), and measured its phosphorylation using the expressed kinase domain of BRI1. As shown in Figure 5C, the wild-type BRI1-KD showed strong kinase activity towards its substrate TTL, but neither bri1-301-KD nor bri1-101-KD had detectable kinase activity.
A similar result was also observed with BAK1 as a native substrate of BRI1 14, 15, 17. Phosphorylated BAK1-KD was barely detectable when BAK1-KD was incubated with bri1-301-KD or bri1-101-KD, and this low phosphorylation activity appears to come from BAK1 autophosphorylation activity, as has been previously described 15, 17 (Figure 5D). By contrast, the phosphorylation of BAK1-KD was apparently enhanced when incubated with BRI1-KD (Figure 5D).
Taken together, we may conclude that the mutation in the bri1-301 kinase domain results in undetectable kinase activity, at least in an in vitro assay system.
Discussion
In this paper, we report the isolation of a weak bri1 allele, bri1-301, using a two-step screening approach. A single amino-acid Gly-to-Ile change in the VIa subdomain of the BRI1 kinase domain produces a defective protein that has undetectable kinase activity. However, the bri1-301 mutant plant shows a mild morphological phenotype when under normal growth conditions, indicating that the loss of kinase activity in bri1-301 protein may not abolish the involvement of BRI1 in regulating plant growth and development.
Many bri1 alleles have been isolated to date and most of them are strong alleles that exhibit severe phenotypes, including extreme dwarfism, a prolonged life span, and complete male sterility 13, 28, 29. Many of them, for example bri1-1, bri1-3, bri1-101, bri1-103, bri1-105, bri1-115, and bri1-117, have defects in the kinase domain 13, 28, and the kinase activity of bri1-101 has been proved to decrease greatly, suggesting that the kinase activity is crucial for the biological function of BRI1 13. However, our studies on bri1-301 raise the question of whether kinase activity is essential for BRI1 in mediating plant growth and development. In fact, there have been some clues to indicate that BRI1 kinase activity might not be indispensable for its function. First, the BRI1 kinase domain is not involved in binding to its ligand BRs 5. Second, the interaction between BRI1 and its interacting proteins BAK1 and BKI1 is independent of kinase activity 14, 19. Third, the severity of the bri1 mutant phenotype does not always correlate with a loss of bri1 kinase activity. For example, bri1-104, a strong bri1 allele, has only 50% reduced kinase activity 18.
BRI1 has been identified to interact directly with several proteins, including the BR signaling positive regulator BAK1 14, 15 and negative regulators TTL 18 and BKI1 19. A possible interpretation of the differences between the bri1-301 and bri1-101 phenotypes is that the Gly-989-Ile mutation in the VIa subdomain in bri1-301 may not only abolish the kinase activity of BRI1 but also alters its conformation, leading to the constitutive release of negative regulatory interactors from BRI1 and/or the recruitment of positive regulatory interactors to BRI1 and thus the activation of BRI1 signaling. However, bri1-101 may be defective in generating or maintaining a functional conformation that is required for BRI1 to interact with its interactors.
Interestingly, bri1-8, another weak bri1 mutant, also carries a conserved amino-acid change from Arg to Gln in the Vla subdomain 29, implying that the Vla subdomain might be important for the BRI1 conformation. bri1-8 and bri1-301 are the only two as-yet-identified weak bri1 alleles that harbor mutations in the BRI1 kinase domain 28. Further investigation of the two weak alleles and characterization of more bri1 mutants that have mutations in the kinase domain will help us to further understand the role of BRI1 kinase activity in its biological functions.
In plants, growth and development are the consequence of an interplay between environmental factors and intrinsic programs. Light is one of the most important environmental factors and regulates many developmental processes throughout the plant life cycle 30, 31. Increasing studies have suggested that light signaling interacts with the BR signaling pathway 32, 33, 34, 35. The findings of undetectable kinase activity in bri1-301 and altered transcription patterns of the light-inducible RbcS1A and Lhcb1.3 genes in the bri1-301 seedlings grown under long-wavelength light and in the dark suggest that the BRI1 kinase domain might function in light signaling, providing an explanation for why BRs interact with light. Therefore, bri1-301 may also serve as a valuable tool in studying the mechanisms underlying the crosstalk between light and BRs.
Materials and Methods
Plant materials
A. thaliana plants were grown on vermiculite saturated with 0.3 × B5 medium 36 under continuous light (80-120 μE/m2 s) at 23 °C as described previously 37, or under light conditions as indicated in the text. For plants grown in petri dishes, seeds were surface-sterilized with 70% (v/v) ethanol for 3 min and 15% commercial bleach solution plus 0.025% Triton X-100 for 15 min, rinsed three times with sterile water, and suspended in 0.1% agar. The sterilized seeds were plated on 0.5 × MS medium 38 containing 0.8% agar and pre-incubated at 4 °C in the dark for 2 days before being cultured under the conditions as indicated.
Isolation and mapping of bri1-301
A. thaliana ecotype Columbia (Col-0) wild-type plants were mutagenized with EMS as described previously 39, and the bri1-301 mutant was isolated using a two-step screening procedure. The mutagenized M2 seeds were germinated and grown at 23 °C for 7 days under long-wavelength light (>460 nm) obtained using a yellow filter (Roscolux #10 Medium Yellow Filter, SSSL Syracuse Scenery & Stage Lighting Co., Inc), and dwarf seedlings were selected and grown under normal white light to produce M3 seeds, which were subsequently subjected to the second-step screening for those showing a normal etiolated phenotype when grown in the dark.
To isolate the gene responsible for the mutant phenotype through a map-based cloning approach, the homozygous mutant plant was pollinated with Ler pollens and an F2 mapping population was constructed using F1 hybrid self-pollination to generate a segregating F2 mapping population. Arabidopsis DNA was isolated from individual mutant plants as described previously 40. Linkage between bri-301 and molecular markers was determined using CAPS and simple sequence length polymorphisms (SSLP) markers 41, 42. To examine allelism, a complementation test was performed between bri1-301 and bri1-101.
Light conditions and treatment with 24-epi-BL
Seedlings were vertically grown on 0.5 × MS medium under continuous white light, continuous long-wavelength light (> 460 nm) and in the dark. Seeds were germinated on 0.5 × MS medium supplemented with various concentrations of 24-epi-BL in the dark.
Real-time PCR analysis
Total RNAs were prepared using the guanidine thiocyanate extraction method as described previously 43. Complementary DNA was reverse transcribed from total RNAs with oligo(dT) as the primer and real-time PCR analysis was carried out according to instructions from the manual of SYBR® GREEN PCR Master Mix using the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). Primers used in the experiments were Tubulin-rt-F and Tubulin-rt-R for Tubulin (5′-ACC TAC TGG TCT GAA GAT GGC AT-3′ and 5′-TTT CTC CTG AAC ATA GCT GTG AAC T-3′); rbcS1A-rt-F and rbcS1A-rt-R for RbcS1A (5′-ACA AGC AAC GGC GGA AGA-3′ and 5′-CGG AAT CGG TAA GGT CAG GA-3′); Lhcb1.3-rt-F and Lhcb1.3-rt-R for Lhcb1.3 (5′-GGA GCT CAA GAA CGG AAG ATT G-3′ and 5′-TCT CTA TCG GTC CCT TAC CAG TG-3′); BAS1-rt-F and BAS1-rt-R for BAS1 (5′-CCC GTT GGC TTC ATA CCG T-3′ and 5′-TTA CAG CGA GTG TCA ATT TGG C-3′); and CPD-rt-F and CPD-rt-R for CPD (5′-CTT ACC GCA AAG CCA TCC A-3′ and 5′-TCA TCA CCA CCA CCG TCA AC-3′).
Kinase assay
To produce the GST and MBP fusion proteins, the BRI1 kinase domain was cloned into the pGEX-6p-1 vector (Amersham Biosciences), and the full-length TTL ORF and the BAK1 kinase domain were cloned into the pMAL-p2X vector (New England Biolabs), respectively. Fusion protein induction and purification were performed according to the manufacturer's protocols (Amersham Biosciences for the GST-BRI1-KD fusion proteins and New England Biolabs for the MBP-TTL and MBP-BAK1-KD fusion proteins). Kinase assays were performed in vitro at 25 °C for 1 h in a 20-μl reaction mixture containing 20 mM Tris, pH 7.5, 100 mM NaCl, 12 mM MgCl2 and 1 μl of [γ-32P]ATP (3000 Ci/mmol; Amersham Biosciences), with approximately the same amount of purified proteins determined by western blot analysis. The kinase reaction was terminated by adding 5 μl of 5 × SDS sample buffer and boiling for 10 min, and then proteins were separated using 8% SDS-PAGE. The gel was exposed to phosphor plates and scanned with a phosphorimager (Typhoon 8600, Amersham Biosciences) to visualize the phosphorylated bands.
References
Clouse SD, Langford M, McMorris TC . A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development. Plant Physiol 1996; 111:671–678.
Clouse SD, Sasse JM . Brassinosteroids: essential regulators of plant growth and development. Annu Rev Plant Physiol Plant Mol Biol 1998; 49:427–451.
Mangelsdorf DJ, Thummel C, Beato M, et al. The nuclear receptor superfamily: the second decade. Cell 1995; 83:835–839.
He Z, Wang ZY, Li J, et al. Perception of brassinosteroids by the extracellular domain of the receptor kinase BRI1. Science 2000; 288:2360–2363.
Kinoshita T, Cano-Delgado A, Seto H, et al. Binding of brassinosteroids to the extracellular domain of plant receptor kinase BRI1. Nature 2005; 433:167–171.
Li J, Chory J . A putative leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. Cell 1997; 90:929–938.
Wang ZY, Seto H, Fujioka S, Yoshida S, Chory J . BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature 2001; 410:380–383.
Chono M, Honda I, Zeniya H, et al. A semidwarf phenotype of barley uzu results from a nucleotide substitution in the gene encoding a putative brassinosteroid receptor. Plant Physiol 2003; 133:1209–1219.
Montoya T, Nomura T, Farrar K, Kaneta T, Yokota T, Bishop GJ . Cloning the tomato curl3 gene highlights the putative dual role of the leucine-rich repeat receptor kinase tBRI1/SR160 in plant steroid hormone and peptide hormone signaling. Plant Cell 2002; 14:3163–3176.
Nomura T, Bishop GJ, Kaneta T, Reid JB, Chory J, Yokota T . The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea. Plant J 2003; 36:291–300.
Sun Y, Fokar M, Asami T, Yoshida S, Allen RD . Characterization of the brassinosteroid insensitive 1 genes of cotton. Plant Mol Biol 2004; 54:221–232.
Yamamuro C, Ihara Y, Wu X, et al. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell 2000; 12:1591–1606.
Friedrichsen DM, Joazeiro CA, Li J, Hunter T, Chory J . Brassinosteroid-insensitive-1 is a ubiquitously expressed leucine-rich repeat receptor Serine/Threonine kinase. Plant Physiol 2000; 123:1247–1256.
Nam KH, Li J . BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell 2002; 110:203–212.
Li J, Wen J, Lease KA, Doke JT, Tax FE, Walker JC . BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell 2002; 110:213–222.
Russinova E, Borst JW, Kwaaitaal M, et al. Heterodimerization and endocytosis of Arabidopsis brassinosteroid receptors BRI1 and AtSERK3 (BAK1). Plant Cell 2004; 16:3216–3229.
Wang X, Goshe MB, Soderblom EJ, et al. Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase. Plant Cell 2005; 17:1685–1703.
Nam KH, Li J . The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1. Plant Cell 2004; 16:2406–2417.
Wang X, Chory J . Brassinosteroids regulate dissociation of BKI1, a negative regulator of BRI1 signaling, from the plasma membrane. Science 2006; 313:1118–1122.
Kim TW, Lee SM, Joo SH, et al. Elongation and gravitropic responses of Arabidopsis roots are regulated by brassinolide and IAA. Plant Cell Environ 2007; 30:679–689.
Cano-Delgado A, Yin Y, Yu C, et al. BRL1 and BRL3 are novel brassinosteroid receptors that function in vascular differentiation in Arabidopsis. Development 2004; 131:5341–5351.
Zhao J, Peng P, Schmitz RJ, Decker AD, Tax FE, Li J . Two putative BIN2 substrates are nuclear components of brassinosteroid signaling. Plant Physiol 2002; 130:1221–1229.
Goda H, Shimada Y, Asami T, Fujioka S, Yoshida S . Microarray analysis of brassinosteroid-regulated genes in Arabidopsis. Plant Physiol 2002; 130:1319–1334.
Kenigsbuch D, Tobin EM . A region of the Arabidopis Lhcb1*3 promoter that binds to CA-1 activity is essential for high expression and phytochrome regulation. Plant Physiol 1995; 108:1023–1027.
Dedonder A, Rethy R, Fredericq H, Van Montagu M, Krebbers E . Arabidopsis rbcS genes are differentially regulated by light. Plant Physiol 1993; 101:801–808.
Turk EM, Fujioka S, Seto H, et al. CYP72B1 inactivates brassinosteroid hormones: an intersection between photomorphogenesis and plant steroid signal transduction. Plant Physiol 2003; 133:1643–1653.
Mathur J, Molnar G, Fujioka S, et al. Transcription of the Arabidopsis CPD gene, encoding a steroidogenic cytochrome P450, is negatively controlled by brassinosteroids. Plant J 1998; 14:593–602.
Vert G, Nemhauser JL, Geldner N, Hong F, Chory J . Molecular mechanisms of steroid hormone signaling in plants. Annu Rev Cell Dev Biol 2005; 21:177–201.
Noguchi T, Fujioka S, Choe S, et al. Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids. Plant Physiol 1999; 121:743–752.
Fankhauser C, Chory J . Light control of plant development. Annu Rev Cell Dev Biol 1997; 13:203–229.
Chory J . Light modulation of vegetative development. Plant Cell 1997; 9:1225–1234.
Nemhauser JL, Maloof JN, Chory J . Building integrated models of plant growth and development. Plant Physiol 2003; 132:436–439.
Kang JG, Yun J, Kim DH, et al. Light and brassinosteroid signals are integrated via a dark-induced small G protein in etiolated seedling growth. Cell 2001; 105:625–636.
Neff MM, Nguyen SM, Malancharuvil EJ, et al. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis. Proc Natl Acad Sci USA 1999; 96:15316–15323.
Li J, Nagpal P, Vitart V, McMorris TC, Chory J . A role for brassinosteroids in light-dependent development of Arabidopsis. Science 1996; 272:398–401.
Gamborg OL, Miller RA, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 1968; 50:151–158.
Mou Z, He Y, Dai Y, Liu X, Li J . Deficiency in fatty acid synthase leads to premature cell death and dramatic alterations in plant morphology. Plant Cell 2000; 12:405–418.
Murashige T, Skoog F . A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962; 15:473–497.
Jacobs M, Dolferus R, Van den Bossche D . Isolation and biochemical analysis of ethyl methanesulfonate-induced alcohol dehydrogenase null mutants of Arabidopsis thaliana (L.) Heynh. Biochem Genet 1988; 26:105–122.
Li J, Zhao J, Rose AB, Schmidt R, Last RL . Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes. Plant Cell 1995; 7:447–461.
Bell CJ, Ecker JR . Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics 1994; 19:137–144.
Konieczny A, Ausubel FM . A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 1993; 4:403–410.
Hu Y, Bao F, Li J . Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway in Arabidopsis. Plant J 2000; 24:693–701.
Acknowledgements
We thank Prof Joanne Chory (The Salk Institute for Biological Studies, USA) for providing the Arabidopsis bri1-101 mutant seeds. This work was supported by grants from the National Natural Science Foundation of China (grant numbers: 30070074, 30330040 and 30570161).
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Xu, W., Huang, J., Li, B. et al. Is kinase activity essential for biological functions of BRI1?. Cell Res 18, 472–478 (2008). https://doi.org/10.1038/cr.2008.36
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DOI: https://doi.org/10.1038/cr.2008.36
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