Figure 6 | Cell Research

Figure 6

From: Kinetochore dynein generates a poleward pulling force to facilitate congression and full chromosome alignment

Figure 6

ZW10 depletion attenuates chromosome oscillation on monoasters. Selected frames of a typical control cell (A) and a ZW10-depleted cell (C) during the first 180 seconds of recording are shown. HEK293T cells transfected as in Figure 2 for 68 h were treated with monastrol for additional 4 h to induce monopolar spindles. H2B-GFP-positive mitotic cells were then imaged at 10-second intervals for 20 min. The innermost side of each of 10 chromosomes is marked with a color dot, and its distance to the cell center (red spot) is measured frame by frame. Data are then presented as a curved line of the same color in (B) or (D), respectively. Also see Supplementary information, Video 67.

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