Figure 4 | Cellular & Molecular Immunology

Figure 4

From: Common genetic heterogeneity of human interleukin-37 leads to functional variance

Figure 4

IL-37 Var2, but not other variants, was heavily polyubiquitinated. HEK293T cells expressing IL37-Var1 and IL37-Var2 were transfected with plasmids encoding Myc-tagged ubiquitin, and empty HEK293T cells were used as a control. At 24 h post-transfection, cells were treated with MG132 (10 μM) for 8 h. IL-37 variants were then immunoprecipitated with anti-Flag gel and immunoblotted with an anti-Ub antibody. A representative blot from three independent experiments is shown.

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