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Engineering humanized mice for improved hematopoietic reconstitution

Cellular & Molecular Immunology volume 9, pages 215–224 (2012)Cite this article

Abstract

Humanized mice are immunodeficient animals engrafted with human hematopoietic stem cells that give rise to various lineages of human blood cells throughout the life of the mouse. This article reviews recent advances in the generation of humanized mice, focusing on practical considerations. We discuss features of different immunodeficient recipient mouse strains, sources of human hematopoietic stem cells, advances in expansion and genetic modification of hematopoietic stem cells, and techniques to modulate the cytokine environment of recipient mice, in order to enhance reconstitution of specific human blood lineage cells. We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications.

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Acknowledgements

We would like to thank Bettina Iliopoulou, Ryan Phennicie, Ed Browne and Peter Bak for their critical reading of the manuscript. This work was supported by funds from the Singapore-MIT Alliance for Research and Technology and the Ragon Institute of MGH, MIT and Harvard (to JC).

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Author notes

  1. Adam C Drake and Qingfeng Chen: These authors contributed equally to this work.

  2. Dr J Chen, Koch Institute for Integrative Cancer Research, MIT, 76-261, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. E-mail: JChen@mit.edu

Authors and Affiliations

  1. Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA

    Adam C Drake & Jianzhu Chen

  2. Interdisciplinary Research Group in Infectious Diseases, Singapore–MIT Alliance for Research and Technology, Singapore

    Qingfeng Chen & Jianzhu Chen

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  1. Adam C Drake
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Drake, A., Chen, Q. & Chen, J. Engineering humanized mice for improved hematopoietic reconstitution. Cell Mol Immunol 9, 215–224 (2012). https://doi.org/10.1038/cmi.2012.6

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Keywords

  • cytokines
  • expansion
  • genetic modification
  • hematopoietic stem cells
  • humanized mice

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