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Promotion of cell proliferation and inhibition of ADCC by cancerous immunoglobulin expressed in cancer cell lines

Cellular & Molecular Immunology volume 9, pages 54–61 (2012)Cite this article

Abstract

To explore the significance of cancerous immunoglobulin (Ig) in cancer cell growth, HeLa cervical cancer cells were stably transfected with small interfering RNA (siRNA) that specifically, efficiently and consistently silences the expression of heavy chain genes of all immunoglobulin isotypes. This stable cell line was used to examine cell viability, colony formation and tumor growth in athymic nude mice. The results of these experiments indicated that siRNA-mediated knockdown of cancerous Ig inhibited cell growth in vitro and suppressed tumor cell growth in immune-deficient nude mice in vivo. Similarly, this siRNA also inhibited the growth of MGC gastric cancer cells and MCF-7 breast cancer cells. Furthermore, the presence of cancerous Ig specifically reduced antibody-dependent cell-mediated cytotoxicity (ADCC) induced by an anti-human epithelial growth factor receptor (EGFR) antibody in a dose-dependent manner, suggesting that the cancerous Ig-Fc receptor interaction inhibits natural killer cell (or NK cell) effector function. The prevalent expression of Ig in human carcinomas and its capacity to promote growth and inhibit immunity might have important implications in growth regulation and targeted therapy for human cancers.

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Acknowledgements

This work was supported by the Major Program of National Natural Science Foundation of China (39830410), National High Technology Research and Development Program (863) of China (2006AA02A404), CMB project (99665) and National Natural Science Foundation of China (30772465).

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Authors and Affiliations

  1. Key Laboratory of Ministry of Education, Key Laboratory of Ministry of Health, Laboratory of Tumor Molecular Biology, Cancer Research Institute, Central South University, Changsha, China

    Ming Li, Hui Zheng, Zhi Duan, Haidan Liu, Duosha Hu & Ya Cao

  2. Department of Immunology, Xiangya Medical College, Central South University, Changsha, China

    Ming Li

  3. The Hormel Institute, University of Minnesota, Minneapolis, MN, USA

    Ann Bode & Zigang Dong

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  1. Ming Li
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Correspondence to Zigang Dong or Ya Cao.

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Li, M., Zheng, H., Duan, Z. et al. Promotion of cell proliferation and inhibition of ADCC by cancerous immunoglobulin expressed in cancer cell lines. Cell Mol Immunol 9, 54–61 (2012). https://doi.org/10.1038/cmi.2011.40

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  • DOI: https://doi.org/10.1038/cmi.2011.40

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