Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Report
  • Published:

Identification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS)

Cellular & Molecular Immunology volume 8, pages 88–91 (2011)Cite this article

Abstract

The multifunctional protein p100 is a vital transcriptional regulator that increases gene transcription by forming a physical bridge between promoter-specific transcription factors and the basal transcription machinery. To identify potential signal transduction pathways in which human p100 acts as a coregulator and to find target promoter regions that may interact with p100, we performed a promoter microarray assay called chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). From this assay, we determined that a set of promoter fragments, including several factors in the transforming growth factor beta (TGF-β) signaling pathway, exhibited interaction with p100. The ChIP-GLAS data were validated by RT-PCR assessing the mRNA expression of various factors in the TGF-β signaling pathway in cell lines.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1

Similar content being viewed by others

References

  1. Tong X, Drapkin R, Yalamanchili R, Mosialos G, Kieff E . The Epstein–Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE. Mol Cell Biol 1995; 15: 4735–4744.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Leverson JD, Koskinen PJ, Orrico FC, Rainio EM, Jalkanen KJ, Dash AB et al. Pim-1 kinase and p100 cooperate to enhance c-Myb activity. Mol Cell 1998; 2: 417–425.

    Article  CAS  PubMed  Google Scholar 

  3. Broadhurst MK, Lee RS, Hawkins S, Wheeler TT . The p100 EBNA-2 coactivator: a highly conserved protein found in a range of exocrine and endocrine cells and tissues in cattle. Biochim Biophys Acta 2005; 1681: 126–133.

    Article  CAS  PubMed  Google Scholar 

  4. Paukku K, Yang J, Silvennoinen O . Tudor and nuclease-like domains containing protein p100 function as coactivators for signal transducer and activator of transcription 5. Mol Endocrinol 2003; 17: 1805–1814.

    Article  CAS  PubMed  Google Scholar 

  5. Valineva T, Yang J, Palovuori R, Silvennoinen O . The transcriptional coactivator protein p100 recruits histone acetyltransferase activity to STAT6 and mediates interaction between the CREB-binding protein and STAT6. J Biol Chem 2005; 280: 14989–14996.

    Article  PubMed  Google Scholar 

  6. Yang J, Aittomaki S, Pesu M, Carter K, Saarinen J, Kalkkinen N et al. Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J 2002; 21: 4950–4958.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. ten Dijke P, Miyazono K, Heldin CH . Signaling inputs converge on nuclear effectors in TGF-beta signaling. Trends Biochem Sci 2000; 25: 64–70.

    Article  CAS  PubMed  Google Scholar 

  8. Massagué J, Wotton D . Transcriptional control by the TGF-beta/Smad signaling system. EMBO J 2000; 19: 1745–1754.

    Article  PubMed  PubMed Central  Google Scholar 

  9. Jakowlew SB . Transforming growth factor-beta in cancer and metastasis. Cancer Metastasis Rev 2006; 25: 435–457.

    Article  CAS  PubMed  Google Scholar 

  10. Ho J, Kong JW, Choong LY, Loh MC, Toy W, Chong PK et al. Novel breast cancer metastasis-associated proteins. J Proteome Res 2009; 8: 583–594.

    Article  CAS  PubMed  Google Scholar 

  11. Kuruma H, Kamata Y, Takahashi H . Staphylococcal nuclease domain-containing protein 1 as a potential tissue marker for prostate cancer. Am J Pathol 2009; 174: 2044–2050.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Tsuchiya N, Ochiai M, Nakashima K, Ubaqai T, Suqimura T, Nakagama H . SND1, a component of RNA-induced silencing complex, is up-regulated in human colon cancers and implicated in early stage colon carcinogenesis. Cancer Res 2007; 67: 9568–9576.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank H. Zhang for his kind technical assistance. This work was supported by grants from the National Basic Research Program (973 Program, 2009CB918903), 863 Project of the Ministry of Science and Technology of China (2007AA02Z115), NSFC (90919032, 30970562, 30670441, 30811130394 and 30870562), Tianjin Municipal Science and Technology Commission (08ZCGHHZ01900 and 08JCYBJC07700), Specialized Fund for the Doctoral Program of Higher Education (20091202110001) and Tianjin Educational Committee Foundation (2008ZD01).

Author information

Author notes

  1. Xin Liu and Lijie Dong: These authors contributed equally to the work.

  2. Dr J Yang Dr Z Yao, Department of Immunology, Tianjin Medical University, Heping District Qixiangtai Road No. 22, Tianjin 300070, China. E-mail: yangj@tijmu.edu.cn, E-mail: yaozhi@tmu.cn

Authors and Affiliations

  1. Department of Immunology, Basic Medical College, Tianjin Medical University, Tianjin, China

    Xin Liu, Lijie Dong, Xuejun Zhang, Baoya Wang, Xinting Wang, Hu Li, Jinyan He, Lin Ge, Zhi Yao & Jie Yang

  2. Tianjin Key Laboratory of Cellular and Molecular Immunology, Tianjin, China

    Xin Liu, Lijie Dong, Xuejun Zhang, Baoya Wang, Xinting Wang, Hu Li, Jinyan He, Lin Ge, Zhi Yao & Jie Yang

  3. Key Laboratory of Educational Ministry of China, Tianjin, China

    Xin Liu, Lijie Dong, Xuejun Zhang, Baoya Wang, Xinting Wang, Hu Li, Jinyan He, Lin Ge, Zhi Yao & Jie Yang

  4. Laboratory of Molecular Immunology, Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin, China

    Xinting Wang, Hu Li & Jie Yang

  5. Department of Ultrasonography, Tianjin Third Central Hospital, Tianjin, China

    Xiang Jing

Authors
  1. Xin Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lijie Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xuejun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Baoya Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xinting Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jinyan He
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lin Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Xiang Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Zhi Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Jie Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, X., Dong, L., Zhang, X. et al. Identification of p100 target promoters by chromatin immunoprecipitation-guided ligation and selection (ChIP-GLAS). Cell Mol Immunol 8, 88–91 (2011). https://doi.org/10.1038/cmi.2010.47

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/cmi.2010.47

Keywords

This article is cited by

Search

Advanced search

Quick links