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Specifically Binding of L-ficolin to N-glycans of HCV Envelope Glycoproteins E1 and E2 Leads to Complement Activation

Cellular and Molecular Immunology volume 6, pages 235244 (2009) | Download Citation

Abstract

L-ficolin, one of lectin families, is a recently identified complement factor that initiates lectin pathway of complement. Little is known about its role in viral hepatitis. In the present study, we found that L-ficolin in serum from 103 patients with hepatitis C virus (HCV), were significantly higher than that in 150 healthy controls. We further found that L-ficolin expressions were significantly increased in vitro study by HCV JFH-1 infected human hepatocyte cell line Huh7.5.1. Investigation of the mechanisms of the L-ficolin action on HCV demonstrated that L-ficolin protein could recognize and bind to envelope glycoproteins E1 and E2 of HCV, activating the lectin complement pathway-mediated cytolytic activity in HCV-infected hepatocyte. This interaction between L-ficolin and HCV E1 and E2 glycoproteins was attributed to the N-glycans of E1 and E2. These findings provide new insights into the biological functions of L-ficolin in clinically important hepatic viral diseases.

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Author notes

    • Jun Liu
    •  & Mohammed A M Ali

    These two authors contribute equally to this work as first authors

Affiliations

  1. The State Key Laboratory of Virology, Department of Immunology and Hubei Province Key Laboratory of Allergy and Immune-related Diseases, Wuhan University School of Medicine, Wuhan 430071, China

    • Jun Liu
    • , Mohammed A M Ali
    • , Yinghua Shi
    • , Yinglan Zhao
    • , Fenglin Luo
    • , Jin Yu
    • , Tian Xiang
    • , Jie Tang
    • , Dongqing Li
    •  & Xiaolian Zhang
  2. Center for Disease Control and Prevention of Wuhan, Wuhan 430071, China

    • Quan Hu
  3. Division of Allergy-Immunology, the Children's Hospital of Philadelphia, Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA, USA

    • Wenzhe Ho
  4. Central Hospital of Enshi Prefecture, Hubei 445000, China

    • Tian Xiang

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Corresponding author

Correspondence to Xiaolian Zhang.

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Received

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DOI

https://doi.org/10.1038/cmi.2009.32

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