Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays

Cellular & Molecular Immunology volume 5, pages 471–474 (2008)Cite this article

Abstract

The wild-type human Fas-associated death domain (FADD) protein was expressed as a His-tag fusion protein in Escherichia coli. Recombinant FADD proteins were purified under the denatured condition. After denatured protein purification, it was refolded and obtained at a yield of about 23 mg/L. Purified FADD exhibited as a homogenous band corresponding to the molecular weight of 31 kDa. Immunization of rabbits against the refolded FADD protein was allowed the production of high titre polyclonal antiserum. This new polyclonal antibody could recognize recombinant FADD protein in Western blot. Immunoreactivity was also observed in immunofluorescence assay. The low cost polyclonal antiserum was applicable to extensive detection of FADD in various immunoassays.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

Author information

Authors and Affiliations

  1. The State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Center of Hepatobiliary Diseases, Nanjing University, Nanjing, 210093, China

    Faiz MMT Marikar, Dingyuan Ma, Jianqiang Ye, Bo Tang, Weijuan Zheng, Jing Zhang, Min Lu & Zichun Hua

Authors
  1. Faiz MMT Marikar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dingyuan Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianqiang Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Bo Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Weijuan Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Min Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zichun Hua
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zichun Hua.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marikar, F., Ma, D., Ye, J. et al. Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays. Cell Mol Immunol 5, 471–474 (2008). https://doi.org/10.1038/cmi.2008.59

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/cmi.2008.59

Keywords

This article is cited by

Search

Advanced search

Quick links