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The novel gene HA117 promotes in vitro and in vivo drug resistance in mouse colon tumor cells

Cancer Gene Therapy volume 24, pages 304–308 (2017)Cite this article

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Abstract

In this study, we characterized the role of HA117, a novel gene we previously identified, in drug resistance in vitro and in vivo. Briefly, CT26 cells expressing HA117 were obtained by infection with a recombinant adenovirus, and tested for drug sensitivity by methyl thiazolyl tetrazolium assay. In addition, the effect of HA117 on drug sensitivity was assessed in CT26 colon tumors transplanted into nude mice. In vitro, expression of HA117 increased the resistance of CT26 cells to 5-fluorouracil 2.75 times (P<0.05). Similarly, expression of HA117 in subcutaneously xenografted CT26 colon cancer significantly lowered its sensitivity to 5-fluorouracil (P<0.05). Collectively, the results suggest that HA117 regulates the development of drug resistance in CT26 cells in vitro and in vivo.

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Acknowledgements

This study was a Surface project of the National Natural Science Foundation of China (No. 30471985). GZ thanks his supervisor, Professor Youhua Xu, for useful advice on writing, and for trying her best to improve the paper.

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Authors and Affiliations

  1. The Infection Department of the Children's Hospital of Chongqing Medical University, The Pediatrics Institution of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Key Laboratory of Pediatrics in Chongqing, CSTC2009CA5002, Chongqing International Science and Technology Cooperation Center for Child Development and Disorders, The Children's Hospital of Chongqing Medical University, Chongqing, China

    Y X Guo, Y H Xu, G H Zheng & X Q Jin

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  1. Y X Guo
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Correspondence to G H Zheng.

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Guo, Y., Xu, Y., Zheng, G. et al. The novel gene HA117 promotes in vitro and in vivo drug resistance in mouse colon tumor cells. Cancer Gene Ther 24, 304–308 (2017). https://doi.org/10.1038/cgt.2017.28

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