Oral verrucous carcinoma (OVC) is a verrucous variant of oral cavity squamous carcinoma (OSCC). The expression of miRNA from OVC and OSCC including their matched normal oral mucosa tissues was profiled through the Affymetrix GeneChip miRNA Arrays. TargetScan and miRanda databases were used to predict the target gene of miRNA-195. The quantitative real-time PCR was applied to validate the expression of miRNA-195. The expression of CDK6 was investigated by the quantitative real-time PCR and immunohistochemistry. In this study, a total of 23 and 35 differentially expressed miRNAs were identified in OVC and OSCC, respectively. Moreover, 44 miRNAs were differentially expressed between OSCC and OVC. In addition, miRNA-195 was significantly decreased in both OVC and OSCC compared to normal oral mucosa. Target gene prediction demonstrated that CDK6 was a potential target gene of miRNA-195. In the quantitative real-time PCR, miR-195 was decreased in OVC and OSCC, which was consistent with the result of miRNA chip analysis. CDK6 was increased in OVC and OSCC, which was opposite to the expression of miRNA-195. In conclusion, miRNA-195 could be the potential diagnosis biomarker and therapy target of OVC.
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Ackerman LV . Verrucous carcinoma of the oral cavity. Surgery 1948; 23: 670–678.
Strojan P, Soba E, Budihna M, Auersperg M . Radiochemotherapy with Vinblastine, Methotrexate, and Bleomycin in the treatment of verrucous carcinoma of the head and neck. J Surg Oncol 2005; 92: 278–283.
Wang Y, Tang Z, Zhao S, Xie X, Yao Z, Liu C et al. Proteomic analysis of human oral verrucous carcinoma. Afr J Biotechnol 2011; 10: 13004–13013.
Kang CJ, Chang JT, Chen TM, Chen IH, Liao CT . Surgical treatment of oral verrucous carcinoma. Chang Gung Med J 2003; 26: 807–812.
Walvekar RR, Chaukar DA, Deshpande MS, Pai PS, Chaturvedi P, Kakade A et al. Verrucous carcinoma of the oral cavity: A clinical and pathological study of 101 cases. Oral Oncol 2009; 45: 47–51.
Shafer WG . Verrucous carcinoma. Int Dent J 1972; 22: 451–459.
Noble-Topham SE, Fliss DM, Hartwick RW, McLachlin CM, Freeman JL, Noyek AM et al. Detection and typing of human papillomavirus in verrucous carcinoma of the oral cavity using the polymerase chain reaction. Arch Otolaryngol Head & Neck Surg 1993; 119: 1299–1304.
Bustos DA, Pavan JV, Carricart SE, Talavera AD, Secchi D, Carrica V et al. Human papillomavirus detection in oral cancer lesions in the city of Cordoba. Revista de la Facultad de Ciencias Medicas 1999; 56: 65–71.
Tian Z, Guo W, Zhang WG . The expression of CD44v6 in oral cancer with different biological behavior. Shanghai J Stomatol 2002; 11: 353–355.
Impola U, Uitto VJ, Hietanen J, Hakkinen L, Zhang L, Larjava H et al. Differential expression of matrilysin-1 (MMP-7), 92 kD gelatinase (MMP-9), and metalloelastase (MMP-12) in oral verrucous and squamous cell cancer. J Pathol 2004; 202: 14–22.
Angadi PV, Krishnapillai R . Cyclin D1 expression in oral squamous cell carcinoma and verrucous carcinoma: correlation with histological differentiation. Oral Surg Oral Med Oral pathol Oral Radiol Endodontics 2007; 103: e30–e35.
Ambros V . The functions of animal microRNAs. Nature 2004; 431: 350–355.
Ambros V . MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell 2003; 113: 673–676.
Bartel DP . MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116: 281–297.
Pasquinelli AE . MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship. Nat Rev Genet 2012; 13: 271–282.
Lovat F, Valeri N, Croce CM . MicroRNAs in the pathogenesis of cancer. Semin Oncol 2011; 38: 724–733.
Palmero EI, de Campos SG, Campos M, de Souza NC, Guerreiro ID, Carvalho AL et al. Mechanisms and role of microRNA deregulation in cancer onset and progression. Genet Mol Biol 2011; 34: 363–370.
Zhao L, Chen X, Cao Y . New role of microRNA: carcinogenesis and clinical application in cancer. Acta Biochim Biophys Sin 2011; 43: 831–839.
Simon GR, Sharma S, Cantor A, Smith P, Bepler G . ERCC1 expression is a predictor of survival in resected patients with non-small cell lung cancer. Chest 2005; 127: 978–983.
Liu L, Chen L, Xu Y, Li R, Du X . microRNA-195 promotes apoptosis and suppresses tumorigenicity of human colorectal cancer cells. Biochem Biophys Res Commun 2010; 400: 236–240.
Li D, Zhao Y, Liu C, Chen X, Qi Y, Jiang Y et al. Analysis of MiR-195 and MiR-497 expression, regulation and role in breast cancer. Clin Cancer Res: Off J Am Assoc Cancer Res 2011; 17: 1722–1730.
Gyugos M, Lendvai G, Kenessey I, Schlachter K, Halasz J, Nagy P et al. MicroRNA expression might predict prognosis of epithelial hepatoblastoma. Virchows Archiv: Int J Pathol 2014; 464: 419–427.
Wang YH, Tian X, Liu OS, Fang XD, Quan HZ, Xie S et al. Gene profiling analysis for patients with oral verrucous carcinoma and oral squamous cell carcinoma. Int J Clin Exp Med 2014; 7: 1845–1852.
Xu T, Zhu Y, Xiong Y, Ge YY, Yun JP, Zhuang SM . MicroRNA-195 suppresses tumorigenicity and regulates G1/S transition of human hepatocellular carcinomacells. Hepatology 2009; 50: 113–121.
Lee RC, Feinbaum RL, Ambros V . The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 1993; 75: 843–854.
Lewis BP, Shih IH, Jones-Rhoades MW, Bartel DP, Burge CB . Prediction of mammalian microRNA targets. Cell 2003; 115: 787–798.
Lu J, Getz G, Miska EA, Alvarez-Saavedra E, Lamb J, Peck D et al. MicroRNA expression profiles classify human cancers. Nature 2005; 435: 834–838.
Lee EJ, Gusev Y, Jiang J, Nuovo GJ, Lerner MR, Frankel WL et al. Expression profiling identifies microRNA signature in pancreatic cancer. Int J Cancer 2007; 120: 1046–1054.
Zhu X, Li Y, Shen H, Li H, Long L, Hui L et al. miR-137 inhibits the proliferation of lung cancer cells by targeting Cdc42 and Cdk6. FEBS Lett 2013; 587: 73–81.
Xiao F, Zhang W, Zhou L, Xie H, Xing C, Ding S et al. microRNA-200a is an independent prognostic factor of hepatocellular carcinoma and induces cell cycle arrest by targeting CDK6. Oncol Rep 2013; 30: 2203–2210.
Konecny GE, Winterhoff B, Kolarova T, Qi J, Manivong K, Dering J et al. Expression of p16 and retinoblastoma determines response to CDK4/6 inhibition in ovarian cancer. Clin Cancer Res: Off J Am Assoc Cancer Res 2011; 17: 1591–1602.
This study was supported by the surface project of the State Natural Science Fund (81671003), the Fundamental Research Funds for the Central Universities of Central South University (2016zzts117) and the doctoral fund of the Ministry of Education (20130162110064).
The authors declare no conflict of interest.
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Deng, Z., Wang, Y., Fang, X. et al. Research on miRNA-195 and target gene CDK6 in oral verrucous carcinoma. Cancer Gene Ther 24, 282–288 (2017). https://doi.org/10.1038/cgt.2017.18