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lncRNA-HIT promotes cell proliferation of non-small cell lung cancer by association with E2F1

Abstract

Lung cancer is the leading cause of cancer-related death around the world. Long noncoding RNA (lncRNA) has pivotal roles in cancer occurrence and development. However, only a few lncRNAs have been functionally characterized. In the present study, we investigated the effects of lncRNA-HIT (HOXA transcript induced by TGFβ) expression on non-small cell lung cancer (NSCLC) cell phenotype with the gain-of-function and loss-of-function assays. We found that ectopic expression or knockdown of lncRNA-HIT markedly increased or decreased NSCLC cell proliferation, respectively. Moreover, we also showed that lncRNA-HIT interacted with E2F1 to regulate its target genes, such as Survivin, FOXM1, SKP2, NELL2 and DOK1. Collectively, our findings indicated that lncRNA-HIT affected the proliferation of NSCLC cells at least in part via regulating the occupancy of E2F1 in the promoter regions of its target genes. The lncRNA-HIT–E2F1 complex may be a potential target for NSCLC treatment.

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Acknowledgements

This research is supported by a grant from the projects of science and technology development plan of Jilin province (No. 20140311050YY) and the projects of health and family planning commission of Jilin province (No. 20152009).

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Correspondence to Y Yang or Z Wang.

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Yu, L., Fang, F., Lu, S. et al. lncRNA-HIT promotes cell proliferation of non-small cell lung cancer by association with E2F1. Cancer Gene Ther 24, 221–226 (2017). https://doi.org/10.1038/cgt.2017.10

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  • DOI: https://doi.org/10.1038/cgt.2017.10

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