Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Original Article
  • Published:

Long noncoding RNA PEG10 regulates proliferation and invasion of esophageal cancer cells

Cancer Gene Therapy volume 22, pages 138–144 (2015)Cite this article

Subjects

Abstract

Long chain noncoding RNAs (LncRNAs), a class of noncoding RNA nucleotides longer than 200 bp, have important roles in a variety of biological processes. Accumulating evidence has confirmed the involvement of LncRNAs in cancer initiation, development and progression. We investigated the expression of LncRNA PEG10 in a cohort of esophageal carcinomas to assess its impact on esophageal cancer cell proliferation, apoptosis and invasion. Quantitaive reverse transcription polymerase chain reaction assays were used to quantify LncRNA PEG10 expression levels in 43 paired EC samples and adjacent non-neoplastic tissues. Cell growth, apoptosis and Transwell invasion assays were used to evaluate the effects of LncRNA PEG10 on esophageal cancer cells. LncRNA PEG10 was expressed at higher levels in esophageal cancer tissues than in adjacent non-neoplastic tissues (P<0.05). This relatively high expression was significantly associated with the occurrence of lymph node metastases (P<0.05). Apoptosis and migration rates were significantly decreased in two esophageal cancer cell lines (EC9706 and KYSE150) transfected with si-LncRNA PEG10 (P<0.05). Downregulation of LncRNA PEG10 decreased the expression of PEG10 (P<0.05). Our results indicated that LncRNA PEG10 is upregulated in esophageal cancer tissues, and its downregulation inhibits proliferation and invasion, and promotes apoptosis in esophageal cancer cells. LncRNA PEG10 may serve as a therapeutic agent in esophageal cancer.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6

Similar content being viewed by others

References

  1. Parkin DM, Bray F, Ferlay J, Pisani P . Global cancer statistics, 2002. CA Cancer J Clin 2005; 55: 74–08.

    Article  Google Scholar 

  2. Holmes RS, Vaughan TL . Epidemiology and pathogenesis of esophageal cancer. Semin Radiat Oncol 2007; 17: 2–9.

    Article  Google Scholar 

  3. Brown LM, Devesa SS, Chow WH . Incidence of adenocarcinoma of the esoph-agus among white Americans by sex, stage, and age. J Natl Cancer Inst 2008; 100: 1184–1187.

    Article  Google Scholar 

  4. Bollschweiler E, Wolfgarten E, Gutschow C, Hölscher AH . Demographic variations in the rising incidence of esophageal adenocarcinoma in white males. Cancer 2001; 92: 549–555.

    Article  CAS  Google Scholar 

  5. Rinn JL, Chang HY . Genome regulation by long noncoding RNAs. Annu Rev Biochem 2012; 81: 145–166.

    Article  CAS  Google Scholar 

  6. Ponting CP, Oliver PL, Reik W . Evolution and functions of long noncoding RNAs. Cell 2009; 136: 629–641.

    Article  CAS  Google Scholar 

  7. Nacher JC . Community structure of non-coding RNA interaction network. J Integr Bioinform 2013; 10: 217.

    Article  Google Scholar 

  8. Du Toit A . Non-coding RNA: RNA stability control by Pol II. Nat Rev Mol Cell Biol 2013; 14: 128.

    Article  CAS  Google Scholar 

  9. Jin G, Sun J, Isaacs SD, Wiley KE, Kim ST, Chu LW et al. Human polymorphisms at long non-coding RNAs (lncRNAs) and association with prostate cancer risk. Carcinogenesis 2011; 32: 1655–1659.

    Article  CAS  Google Scholar 

  10. Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ et al. Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature 2010; 464: 1071–1076.

    Article  CAS  Google Scholar 

  11. Loewer S, Cabili MN, Guttman M, Loh YH, Thomas K, Park IH et al. Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells. Nat Genet 2010; 42: 1113–1117.

    Article  CAS  Google Scholar 

  12. Wang J, Liu X, Wu H, Ni P, Gu Z, Qiao Y et al. CREB upregulates long non-coding RNA, HULC expression through interaction with microRNA-372 in liver cancer. Nucleic Acids Res 2010; 38: 5366–5383.

    Article  CAS  Google Scholar 

  13. Khaitan D, Dinger ME, Mazar J, Crawford J, Smith MA, Mattick JS et al. The melanoma-upregulated long noncoding RNA SPRY4-IT1 modulates apoptosis and invasion. Cancer Res 2011; 71: 3852–3862.

    Article  CAS  Google Scholar 

  14. Yang F, Zhang L, Huo XS, Yuan JH, Xu D, Yuan SX et al. Long noncoding RNA high expression in hepatocellular carcinoma facilitates tumor growth through enhancer of zeste homolog 2 in humans. Hepatology 2011; 54: 1679–1689.

    Article  CAS  Google Scholar 

  15. Cui Z, Ren S, Lu J, Wang F, Xu W, Sun Y et al. The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor. Urol Oncol 2013; 31: 1117–1123.

    Article  CAS  Google Scholar 

  16. Wang Y, Chen W, Yang C, Wu W, Wu S, Qin X et al. Long non-coding RNA UCA1a(CUDR) promotes proliferation and tumorigenesis of bladder cancer. Int J Oncol 2012; 41: 276–284 14.

    CAS  PubMed  Google Scholar 

  17. Wilusz JE, Sunwoo H, Spector DL . Long noncoding RNAs: functional surprises from the RNA world. Genes Dev 2009; 23: 1494–1504.

    Article  CAS  Google Scholar 

  18. Ono R, Kobayashi S, Wagatsuma H, Aisaka K, Kohda T, Kaneko-Ishino T et al. A retrotransposonderived gene, PEG10, is a novel imprinted gene located on human chromosome 7q21. Genomics 2001; 73: 232–237.

    Article  CAS  Google Scholar 

  19. Clark MB, Janicke M, Gottesbuhren U, Kleffmann T, Legge M, Poole ES et al. Mammalian genePEG10 expresses two reading frames by high efficiency -1frameshifting in embryonic-associated tissues. J Biol Chem 2007; 282: 37359–37369.

    Article  CAS  Google Scholar 

  20. O'Doherty AM, O'Shea LC, Fair T . Bovine DNA methylation imprints are established in an oocyte size-specific manner, which are coordinated with the expression of the DNMT3 family proteins. Biol Reprod 2012; 86: 67.

    Article  Google Scholar 

  21. Jia HL, Ye QH, Qin LX, Budhu A, Forgues M, Chen Y et al. Gene expression profiling reveals potential biomarkers of human hepatocellular carcinoma. Clin Cancer Res 2007; 13: 1133–1139.

    Article  CAS  Google Scholar 

  22. Ip WK, Lai PB, Wong NL, Sy SM, Beheshti B, Squire JA et al. Identification of PEG10 as a progression related biomarker for hepatocellular carcinoma. Cancer Lett 2007; 250: 284–291.

    Article  CAS  Google Scholar 

  23. Liu Z, Yang Z, Liu D, Li D, Zou Q, Yuan Y et al. TSG101 and PEG10 are prognostic markers in squamous cell/adenosquamous carcinomas and adenocarcinoma of the gallbladder. Oncol Lett 2014; 7: 1128–1138.

    Article  Google Scholar 

  24. Deng X, Hu Y, Ding Q, Han R, Guo Q, Qin J et al. PEG10 plays a crucial role in human lung cancer proliferation, progression, prognosis and metastasis. Oncol Rep 2014; 32: 2159–2167.

    Article  CAS  Google Scholar 

  25. Saad Y, El-Serafy M, Eldin MS, Abdellatif Z, Khatab H, Elbaz T et al. New genetic markers for diagnosis of hepatitis C related hepatocellular carcinoma in Egyptian patients. J Gastrointestin Liver Dis 2013; 22: 419–425.

    PubMed  Google Scholar 

  26. Henke C, Ruebner M, Faschingbauer F, Stolt CC, Schaefer N, Lang N et al. Regulation of murine placentogenesis by the retroviral genes Syncytin-A, Syncytin-B and Peg10. Differentiation 2013; 85: 150–160.

    Article  CAS  Google Scholar 

  27. Xiong J, Qin J, Zheng Y, Peng X, Luo Y, Meng X . PEG10 promotes the migration of human Burkitt's lymphoma cells by up-regulating the expression of matrix metalloproteinase-2 and -9. Clin Invest Med 2012; 35: E117–E125.

    Article  CAS  Google Scholar 

  28. Chen H, Sun M, Zhao G, Liu J, Gao W, Si S et al. Elevated expression of PEG10 in human placentas from preeclamptic pregnancies. Acta Histochem 2012; 114: 589–593.

    Article  CAS  Google Scholar 

  29. Huang W, Long N, Khatib H . Genome-wide identification and initial characterization of bovine long non-coding RNAs from EST data. Anim Genet 2012; 43: 674–682.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Basic Medical Sciences, Zhengzhou University, Zhengzhou, People’s Republic of China

    W Zang, J Huang, M Li, Y Wang, Y Du, X Chen & G Zhao

  2. Department of Hemato-tumor, The First Affiliated Hospital of Henan University of TCM, Zhengzhou, People’s Republic of China

    T Wang

Authors
  1. W Zang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y Du
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. X Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. G Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G Zhao.

Ethics declarations

Competing interests

The authors declare no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zang, W., Wang, T., Huang, J. et al. Long noncoding RNA PEG10 regulates proliferation and invasion of esophageal cancer cells. Cancer Gene Ther 22, 138–144 (2015). https://doi.org/10.1038/cgt.2014.77

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/cgt.2014.77

This article is cited by

Search

Advanced search

Quick links