Recent studies have demonstrated that small double-stranded RNAs (dsRNAs) complementary to the promoter region of target genes enhance the expression of those genes following transfection into cells. Here we show that expression of the matrix metalloproteinase (MMP) inhibitor RECK is activated in the cultured tumor cell lines by transfection with dsRNA complementary to the promoter of the RECK gene, leading to suppression of the expression of MMPs and it inhibited tumor cell invasion. These results support the suggestion that dsRNA complementary to the promoter region of tumor suppressor genes would have potential as a novel antitumor agent.
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This study was supported by the Grant-in-Aid for challenging Exploratory Research from the Japan Society of Promotion of Sciences, grants from the Ministry of Health, Labor and Welfare of Japan, and a grant from Suzuken Memorial foundation (to FS).
The authors declare no conflict of interest.
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Sakurai, F., Nanjo, Y., Okamoto, S. et al. Upregulation of RECK gene expression by small double-stranded RNA targeting the promoter region. Cancer Gene Ther 21, 164–170 (2014) doi:10.1038/cgt.2014.12
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