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Oral contrast enhances the resolution of in-life NIS reporter gene imaging


Sodium iodide symporter (NIS) reporter gene imaging is an excellent technology for noninvasive cell fate determination in living animals unless the NIS-transduced cells reside in perigastric organs such as the spleen, liver, diaphragm, omentum, pancreas, perigastric lymph nodes or perigastric tumor deposits. Here we report that orally administered barium sulfate enhances CT definition of the stomach, masks background gamma ray emissions from the stomach and enhances signal detection from radiotracer uptake in NIS-transduced organs.

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We thank Suzanne Greiner (Mayo Clinic) for her veterinary expertise, Michael K O’Connor (Radiology, Mayo Clinic) for critical review of the manuscript, Tracy Decklever and Brad Kemp (Radiology, Mayo Clinic) of the Mayo Clinic Small Animal Imaging Core. This work was supported by the Mayo Foundation and grants from the National Institutes of Health (R01CA129193, R01CA100634, R01CA129966).

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Correspondence to K-W Peng.

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Competing interests

SJR and KWP are co-founders of Imanis Life Sciences, a reporter gene imaging company. SJR, KWP and Mayo Clinic own equity in Imanis.

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Supplementary Information accompanies the paper on Cancer Gene Therapy website

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Suksanpaisan, L., Pham, L., McIvor, S. et al. Oral contrast enhances the resolution of in-life NIS reporter gene imaging. Cancer Gene Ther 20, 638–641 (2013).

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  • NIS
  • perigastric
  • reporter gene
  • oral contrast
  • barium sulfate

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