Modulation of tumorigenesis by the pro-inflammatory microRNA miR-301a in mouse models of lung cancer and colorectal cancer

[This corrects the article DOI: 10.1038/celldisc.2015.5.].


Introduction
Tumor-promoting inflammation is a recognized hallmark of cancer, as neoplastic epithelial cells recruit ostensibly normal immune inflammatory cells and other stromal cells to create a tumor-promoting microenvironment. Both nuclear factor-κB (NF-κB) and Stat3 activation are important links between inflammation and cancer by regulating distinct functions in cancer cells and surrounding nontumorigenic cells. Within malignant and pre-malignant cells, NF-κB and Stat3 exert important oncogenic functions by promoting tumor cell survival and proliferation and producing cytokines to create a tumor-promoting microenvironment. At the same time, tumor-infiltrating immune cells with activated NF-κB and Stat3 produce cytokines that foster tumor cell growth and attract additional immune cells that sustain tumor-associated inflammation [1].
The study of inflammation-associated lung and colon carcinogenesis has revealed the role of NF-κB activation in both tumor cells and tumor-associated myeloid cells. For example, loss of the NF-κB subunit p65/RelA in mice reduces Kras-driven cellular transformation and lung tumor development [2,3]. It is demonstrated that NF-κB is activated in both tumor cells and myeloid cells within lung tumors in Kras LA2 mice, but its activation in myeloid cells has a more critical role in tumor progression than in epithelialderived tumor cells [4]. In colon tumorigenesis induced by azoxymethane and dextran sodium sulfate (AOM/ DSS), deletion of IKKβ-a key component in NF-κB activation-in intestinal epithelial cells does not decrease inflammation or affect tumor size, yet it leads to a marked decrease in tumor incidence. However, deleting IKKβ in myeloid cells results in a significant decrease in tumor size [5]. Similar to NF-κB, persistent activation of Stat3 is also critical to inflammationassociated tumorigenesis in the lung [6] and colon [7]. Overexpression of Stat3 in alveolar epithelial cells induces inflammation and adenocarcinomas in mouse lung [6], whereas loss of Stat3 in pancreatic or intestinal epithelial cells inhibits tumorigenesis [7,8]. Induced Stat3 ablation in hematopoietic cells in adult mice modulates cytokine production to inhibit tumorspecific immune responses and inflammation [9,10]. These cytokines, including interleukin (IL)-6, tumor necrosis factor-α (TNF-α), and IL-17, are transactivated by NF-κB and/or Stat3 and are direct mediators in the tumor microenvironment, stimulating tumor and immune cell proliferation, and fostering tumor growth and progression. High expression levels of these cytokines are found in different types of epithelial-derived tumors and is associated with poor prognosis [11].
Over 1000 miRNA genes have been identified in the human genome, rendering them one of the most abundant classes of regulatory molecules. Despite that a substantial number of miRNAs modulate NF-κB or Stat3 activation [12,13], there have been few investigations of miRNAs using animal models to appraise their roles in the pathobiology of inflammatory-associated cancer. MicroRNA-301a (miR-301a) is overexpressed in lung cancer [14][15][16] and colon cancer [17], and previous studies indicated that miR-301a is a potential oncogenic miRNA and contributes to tumor formation (18)(19)(20)(21). Inhibition of miR-301a reduces anchorageindependent colony formation of lung cancer cells [18] and attenuates xenograft pancreatic tumor growth [19]. In gastric cancer, overexpression of miR-301a promotes cell proliferation [20]. Of strongest relevance to inflammation at the molecular level, miR-301a is an activator of both NF-κB and Stat3 in tumor cells [19], and in inflammatory T cells [21]. Furthermore, NF-κB transactivates the miR-301a gene [19]. Collectively, these in vitro data implicate that miR-301a promotes inflammation during tumorigenesis. In the present study, we report that miR-301a deficiency inhibits the activation of both NF-κB and Stat3, and protects mice from tumorigenesis at the two major cancer sites. Through these genetic and chemical-induced carcinogenesis models, our findings suggest that miR-301a is a key positive regulator of inflammation, and that it can be attenuated to reduce the burden of cancer mortality.

miR-301a deficiency suppresses Kras-driven lung tumorigenesis
To explore the role of miR-301a in inflammation and tumorigenesis, we performed genetic targeting of the miR-301a gene, which is located between exons 1 and 2 of the Ska2 gene in the mouse genome (Supplementary Figure S1A). To delete miR-301a in mouse embryonic stem (ES) cells, we generated a vector that replaces the precursor to miR-301 with a neomycin (NEO)-resistance expression cassette flanked by FLP recombinase target sequences. Targeted ES colonies were selected for the presence of NEO (Supplementary Figure S1B). The ES cells and all mice used were on a pure C57bl/6J (B6) genetic background. To reduce the risk of altering the expression of the host gene Ska2, the NEO resistance cassette was removed by crossing miR-301 +/NEO mice with mice carrying the Flpe recombinase gene (Supplementary Figure S1C). Heterozygous (miR-301a +/ − ) mice were intercrossed to obtain homozygous (miR-301a −/− ) mice, which were born at the predicted Mendelian frequency and were viable, with no obvious developmental defects or physiological abnormalities.
We measured the expression levels of miR-301a in an experimental mouse model of non-small cell lung cancer using Kras LA2 mice with a targeted and latent mutant Kras G12D allele. These animals develop multifocal lung tumors with 100% penetrance, and, less frequently, develop thymic lymphomas and skin papillomas [22]. miR-301a was highly expressed both in lung tumors and in spleens in Kras LA2 mice compared with wild-type (WT) mice as determined by quantitative real-time PCR (qPCR; Figure 1a). Of note, the expression level of miR-301a in spleens represented a 9.4-fold upregulation, whereas that in lung tumors represented a 2.6-fold upregulation. To investigate the role of miR-301a in lung tumor development, we crossed the miR-301a −/− mice with Kras LA2 mice. Compared with Kras LA2 mutant mice, miR-301a −/− ;Kras LA2 mice showed significantly fewer lung tumors at 18 weeks, either by visual inspection (Figure 1b) or by histological analysis with hematoxylin and eosin staining (Figures 1c-e). In addition, smaller tumors developed in miR-301a −/− ;Kras LA2 mice ( Figure 1f). Next, we analyzed whether deletion of miR-301a alters the tumor spectrum. By the age of 18 weeks, all miR-301a −/− ;Kras LA2 mice developed lung tumors but showed a decreased incidence of thymic lymphomas (21.4% compared with 32.3% in Kras LA2 mice) and skin papillomas (7.1% compared with 17.6%; Figure 1g). In agreement with a reduced tumor burden, miR-301a −/− ;Kras LA2 mice showed significantly increased survival rates compared with their Kras LA2 littermates (Figure 1h; 261 ± 98 days versus 165 ± 64 days; Po0.0001). miR-301a regulates NF-κB and Stat3 activation in lung tumorigenesis miR-301a overexpression was associated with inhibition of apoptosis and augmentation of cell proliferation in breast cancer cells and xenografts [23]. We evaluated cell proliferation and apoptosis in lung tumors. There were significantly fewer Ki67 + tumor cells (Figures 2a and b) in miR-301a −/− ;Kras LA2 mice compared with Kras LA2 mice. However, there was no significant difference in cell apoptosis between miR-301a −/− ;Kras LA2 tumors, and Kras LA2 control tumors, as judged by terminal deoxynucleotidyl transferase dUTP nick end labeling terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL); Figures 2c and d). We examined NF-κB and Stat3 activation in lung tumors. NF- Values are means ± s.d. **P ⩽ 0.01 indicates the significance of the differences between experimental groups.
Xiaodong Ma et al. 3 κB and Stat3 activation were evaluated using immunostaining with antibodies against phospho-p65 or phospho-Stat3. Consistent with previous reports, both NF-κB and Stat3 were constitutively activated in Kras LA2 lung tumors [2,4], as judged by increased phosphorylation of RelA and Stat3. However, Modulation of tumorigenesis by miR-301a 4 significantly less NF-κB and Stat3 activation was found in miR-301a −/− ;Kras LA2 lung tumors compared with Kras LA2 lung tumors (Figures 2e and f).
To better understand how inhibition of NF-κB and Stat3 activation by miR-301a deletion leads to attenuated tumor growth, we first measured the expression of cytokine genes that are transactivated by NF-κB and/or Stat3 in lung tumors from miR-301a −/− ;Kras LA2 , and Kras LA2 mice at 18 weeks. IL-6 and TNF-α showed significantly lower expression in the lung tumors from miR-301a −/− ;Kras LA2 mice compared with those from Kras LA2 mice (Figure 2g). The expression levels of IL-17, IL-1β, Interferon gamma (IFNG), IL-22, and IL-10 (Supplementary Figures S2A-E) showed no significant differences. Next, we measured IL-6 and TNFα expression by using immunohistochemistry staining. As shown in Figure 2h, Both IL-6 and TNFα were constitutively activated in peritumoral lung tissue in Kras LA2 mice, yet such activation was markedly inhibited in miR-301a −/− ;Kras LA2 peritumoral lung tissue, mainly in infiltrating inflammatory cells.
To further determine whether miR-301a deletion reduces Kras-driven cell transformation, we used tumor cells transformed from WT and miR-301a −/− mouse embryonic fibroblasts cells (MEFs) using retroviruses expressing SV40 large T antigen and H-Ras. Transformed miR-301a −/− MEFs proliferated at a slower rate than WT counterparts (Supplementary Figure S3A). However, there was no significant difference in anchorage-independent colony formation between transformed WT and miR-301a −/− cells (Supplementary Figures S3B and C). We then examined the tumorigenicity of transformed WT and miR-301a −/− MEFs by subcutaneously injecting them into athymic-immunodeficient mice. As shown in Supplementary Figure S3D, tumors in nude mice inoculated with WT or miR-301a −/− MEFs showed no significant difference in growth.
NF-κB is activated upon TNF-α treatment, and Stat3 is activated upon IL-6 treatment in various cell types, including tumor cells and immune cells. Constitutive activity of NF-κB was maintained by activated Stat3 in tumor cells and tumor-associated immune cells [24], and NF-κB activation was also induced by IL-6 [25]. We sought to determine whether genetic deletion of miR-301a inhibits NF-κB or Stat3 activation with IL-6 or TNF-α stimulation in MEFs, tumor cells transformed from MEFs with H-Ras and SV40 large T antigen, CD4 + T cells, splenic CD11b + cells (including monocytes and macrophages), and CD11c + cells (including dendritic cells). We found that IL-6 activated both NF-κB and Stat3 in transformed cells, and three types of immune cells, and such activation was markedly inhibited by deletion of miR-301a (Supplementary Figure S4A-E). Furthermore, NF-κB activation by TNF-α was inhibited by miR-301a deficiency in MEFs, CD11b + cells, and CD11c + cells but not in transformed cells or in CD4 + T cells (Supplementary Figures S4A-E). These data suggest that miR-301a-mediated NF-κB and Stat3 activation in vitro is dependent on the cellular context.

miR-301a deficiency reduces chemical-induced colon tumorigenesis
Colitis-associated cancer (CAC) is a type of colon cancer that appears in patients suffering from ulcerative colitis, a chronic inflammatory bowel disease. Mouse models of CAC induced by the carcinogen AOM and repeated administration of the irritant DSS were the first in which it was demonstrated that inflammation is essential to carcinogen-induced tumorigenesis. We studied the role of miR-301a in inflammation and colon cancer by treating WT and miR-301a −/− mice with one dose of AOM followed by three cycles of oral DSS administration. All WT mice developed colon tumors, but a quarter of miR-301a −/− mice developed no tumors. All colon tumors developed in the middle and the distal colon (Figure 3a), and loss of miR-301a markedly inhibited tumor growth (Figures 3a and b) and resulted in fewer tumors per animal ( Figure 3c). The average tumor size was significantly smaller in miR-301a −/− mice (Figure 3d), and miR-301a −/− mice exhibited a lower frequency of large adenomas than WT mice ( Figure 3e). Next, we performed Ki67 staining and found that there was significantly decreased cell proliferation in miR-301a −/− mice compared with WT controls (Figures 3f and g). In agreement with the previous role of miR-301a in lung tumorigenesis, immunohistochemical staining revealed downregulated Stat3 and NF-κB activation in miR-301a −/− mice treated with AOM and DSS ( Figures  3h and i). These results demonstrate that miR-301a deficiency inhibits colon carcinogenesis in mice by downregulating Stat3 and NF-κB activation.

miR-301a deficiency decreases the severity of DSS-induced colitis
Inhibition of inflammation leads to reduction of tumorigenesis in the CAC mouse model [5,26], so we sought to determine whether attenuated tumorigenesis in miR-301a −/− mice is due to suppressed intestinal inflammation. We first measured the expression level of miR-301a in WT mice with drinking water containing 3.5% DSS for 5 days. Compared with controls, the ΔCt  values of miR-301a normalized to a control RNA (snoRNA292) were significantly lower in colon from mice with 3.5% DSS (P = 0.008; Figure 4a). The difference in ΔCt values represented a 2.8-fold upregulation (using mean values) of miR-301a during colitis. Next, we evaluated the severity of colitis in WT and miR-301 −/− mice treated with 3.5% DSS. Compared with WT controls, miR-301a −/− mice showed significantly less body weight loss ( Figure 4b) and reduced colon shortening (Figures 4c and d). There were fewer incidences of bloody stool, rectal bleeding, and anal prolapse in miR-301a −/− mice than in WT mice. Furthermore, histopathological assessment of colitis severity scores showed less-damaged colonic mucosa in miR-301a −/− mice than in WT mice when mucin depletion, epithelial degeneration, and epithelial cellular necrosis were evaluated (Figures 4e and f).
Intestinal epithelial cell proliferation, as judged by Ki67 staining, was significantly elevated in WT mice upon DSS treatment, while miR-301a deletion resulted in fewer proliferating cells than in WT controls (Figures 4g and h). We measured NF-κB and Stat3 activation in WT and miR-301a −/− mice following DSS exposure. Both NF-κB and Stat3 were constitutively activated in treated WT colon, yet such activation was markedly inhibited in miR-301a −/− colon (Figures 5a and b). Careful examination revealed that NF-κB and Stat3 activation were inhibited in both epithelial cells and infiltrating immune cells with miR-301a deletion. The mRNA levels of inflammatory cytokines such as IL-6, TNF-α, and IL-17 were elevated in WT colon treated with DSS, whereas they were downregulated in miR-301a −/− colon compared with WT controls (Figure 5c). As the production of these cytokines secreted from colon mucosa increased progressively during DSS treatment [27], we next prepared serum from mice treated with DSS. A marked increase in IL-6, TNF-α, and IL-17 levels was observed in WT mice, yet their levels decreased significantly in miR-301a −/− mice (Figures  5d-f). Furthermore, we also isolated colon tissues from mice after DSS treatment and examined cytokine secretion ex vivo. Secretion of IL-6, TNF-α, and IL-17 decreased with miR-301a deletion (Figures 5g-i). These data suggest that miR-301a deletion reduces NF-κB and Stat3 activation, downregulating the expression of cytokines during colon inflammation.

Deletion of miR-301a in bone marrow-derived cells inhibits colon inflammation and tumorigenesis
Bone marrow transplantation allows one to obtain chimeric mice with the same epithelial cells but different immune cells. Owing to the lethal doses of radiation, healthy mice are required as the host. We therefore chose the colon inflammation and tumorigenesis models rather than the lung or pancreas tumorigenesis models in which mice develop neoplasia at earlier ages. We performed bone marrow transplantation using WT mice (with CD45.1) as recipients and bone marrow from either miR-301a −/− or WT mice as donors (both with CD45.2). Four weeks after bone marrow transplantation, mice were analyzed for hematopoietic recovery and the level of chimerism. Flow cytometry analysis of peripheral blood mononuclear cells for the presence of donor-derived leukocytes expressing the CD45.2 isoform showed high levels of chimerism in recipient mice (92.3% in WT mice and 93.3% in miR-301a −/− mice). Both groups of mice were administered DSS, and WT mice transplanted with miR-301a −/− hematopoietic cells showed less-severe colitis, as indicated by less body weight loss, reduced colon shortening, fewer histology alterations, and lower colitis scores (Figures 6a-e). The sustained phosphorylation of NF-κB and Stat3 were reduced when miR-301a −/− bone marrow cells were transplanted into WT mice (miR-301a −/− → WT; Figure 6f). We next treated separate groups of transplanted mice with both AOM and DSS, and found that tumor incidence, total tumor size, and the frequency of larger adenomas were significantly reduced in WT mice with miR-301a −/− bone marrow (Figures 6g-j). In addition, the activation of NF-κB and Stat3 in WT mice transplanted with miR-301a −/− hematopoietic cells was reduced when mice were treated with AOM and DSS ( Supplementary  Figures S5A and B). Concomitantly, there was less IL-6 and TNFα expression in infiltrating macrophages and less IL-17 expression in T cells in the colonic mucosa of WT mice with miR-301a −/− bone marrow than that of WT mice with WT bone marrow ( Supplementary Figures S5C and D). These data support the conclusion that attenuated colon tumor development in miR-301a −/− mice was due to miR-301a function in hematopoietic cells rather than in intestinal epithelial cells, underscoring the importance of miR-301a in immune cells during colon tumorigenesis.

miR-301a deficiency in host cells inhibits tumorigenesis in murine syngeneic xenograft models
We assessed the role of miR-301a in tumor stroma using syngeneic xenograft models. Sex-and agematched WT and miR-301a −/− littermates were challenged with Lewis lung carcinoma (LLC1) cells. Tumors grown in miR-301a −/− mice were significantly smaller than those in WT mice (Figure 7a). Consistent with reduced tumor growth, there was a significant decrease in proliferation at the tumor-stroma border as determined by Ki67 staining in miR-301a −/− mice compared with WT mice (Figure 7b). This observation was not limited to LLC1 cells, as attenuated tumor Modulation of tumorigenesis by miR-301a 8 Cell Discovery | www.nature.com/celldisc growth was also found when miR-301a −/− mice were inoculated with B16 melanoma cells ( Supplementary  Figures S6A and B). Collectively, these data imply that loss of miR-301a in the tumor microenvironment but not in tumor cells (Supplementary Figure S3) impedes tumorigenesis in murine xenografts.
To validate the function of miR-301a in tumorinfiltrating immune cells, we isolated CD11b + myeloid and CD4 + T cells from tumors developed in WT mice xenografted with LLC1 cells or from spleens from xenografted or uninoculated mice. We found that miR-301a was highly expressed in tumor-associated CD11b + myeloid cells (12.8-fold; Supplementary Figure S7A) and CD4 + T cells (2.7-fold; Supplementary Figure S7B) compared with the splenic CD11b + and CD4 + counterparts from unxenografted mice. We next measured the activation of NF-κB and Stat3 in immune cells from spleens of WT and

Modulation of tumorigenesis by miR-301a
miR-301a −/− mice, with or without LLC1 injection, or from LCC1-xenografted tumors using flow cytometry. As shown in Figure 7c, splenic cells from WT mice subcutaneously injected with LLC1 tumor cells displayed increased NF-κB and Stat3 activation, and such activation was significantly inhibited in miR-301a −/− mice. Similar patterns were observed in the tumorinfiltrating immune cells isolated from xenografted tumors. When tumor-associated immune cells were separately measured, reduced NF-κB and Stat3 activation were found in macrophages (Gr1 -CD11b + ), myeloid-derived suppressor cells (Gr1 + CD11b + ), dendritic cells (CD11c + ), and CD4 + T cells (CD4 + ) isolated from tumors in miR-301a −/− mice compared with WT controls (Supplementary Figure S7C). We also performed western blot analysis with protein extracts from LCC1 tumors, and found higher IκBα protein levels and reduced Stat3 phosphorylation, an indication of reduced activation of both NF-κB and Stat3, in tumors from miR-301a −/− mice than those from WT mice. This is likely due to elevated expression of Nkrf and Pias3, respective inhibitors to NF-κB and Stat3, and validated miR-301a target genes [19,21] (Figure 7d). We determined the mRNA levels of Nkrf and Pias3 in CD11b + cells and CD4 + T cells isolated from LLC1 tumors using qPCR, and we found that the both Nkrf and Pias3 were upregulated in CD11b + cells and CD4 + T cells isolated from tumors grown in miR-301a −/− mice compared with those from WT mice (Supplementary Figure S7D). Finally, we examined whether miR-301a regulates cytokine gene expression in LLC1xenografted tumors in WT and miR-301a −/− mice. IL--6, TNF-α, and IL-17 (Figure 7e), but not IFNG, IL--10, IL-12A, IL-22, and IL-23 (Supplementary Figure  S7E), were downregulated in miR-301a −/− mice challenged with LLC1 compared with WT controls. Taken together, these results support that miR-301a is upregulated in tumor-associated immune cells during tumorigenesis and that miR-301a activates both NF-κB and Stat3 through targeting Nkrf and Pias3 and elevates cytokine production and tumor cell proliferation.

Discussion
Among the estimated 570 000 annual cancer deaths in the United States, cancers at the sites of lung and colon account for 157 000 and 51 000 deaths, respectively; combined, they cause~30-40% of all cancerrelated deaths [28]. The two types of deadliest cancers share certain features. First, Kras is one of the most frequently mutated oncogenes in both lung and colon cancers; it is mutated in~25-50% of lung cancers and 50% of colon cancers, and Kras-activation-based mouse models have been developed to recapitulate the pathobiology of lung cancer. In rodent colon carcinogenesis induced by AOM/DSS, Kras mutation is observed in most colon tumors from rats and less frequently in those from mice [29]. Kras activation constitutively induces the activation of NF-κB and Stat3 [2,30,31]. Second, inflammation is a significant risk factor for these cancers. Epidemiological studies have consistently linked the presence of chronic obstructive pulmonary disease to the development of lung cancer [32]. Long-standing ulcerative colitis predisposes to the development of CAC [33]. Third, the pro-inflammatory miRNA miR-301a, which activates both NF-κB and Stat3, is overexpressed in these two types of cancer. In the present work, we investigated the role of miR-301a in tumorigenesis at the sites of lung using a genetic model and of colon using a chemical-induced model. Our results show that deletion of miR-301a in Kras LA2 mice reduces multiplicity, incidence, and size of lung tumors, and miR-301a deficiency increases animal survival due to the moderated tumor burden. We also demonstrated that miR-301a-null mice are protected from DSS-induced colitis and AOM/DSS-induced carcinogenesis. These studies imply that miR-301a overexpression contributes to the etiology of the two deadliest solid malignancies.
Both NF-κB and Stat3 control the dialog between the tumor cell and its microenvironment by regulating several cytokine and inflammatory immune mediators. IL-6 and TNF-α are two pro-inflammatory cytokines that contribute to pronounced immune cell infiltration and create a tumor-promoting microenvironment; inactivated NF-κB or Stat3 in myeloid cells reduced tumor growth and decreased the production of IL-6 and TNF-α in the tumor microenvironment [34][35][36]. IL-17 is a pleiotropic pro-inflammatory cytokine and is generally considered to promote non-small cell lung cancer and colon cancer [37][38][39][40], although it was found to inhibit tumorigenesis in a xenograft model using MC38 murine colon cancer cells [41]. Thus, the net effects of IL-17 in tumorigenesis vary and are dependent on the context. Nonetheless, NF-κB, Stat3, and these three cytokines are interconnected: IL6 activates Stat3, TNF-α and IL-17 activate NF-κB, while NF-κB and Stat3 control the production of all three cytokines with the exception of TNF-α, which is not transactivated by Stat3. In our study, we found that there is higher expression of IL-6, TNF-α, and IL-17 during tumorigenesis at the sites of lung and colon, excluding IL-17 in lung tumors, where fewer CD4 + T cells are found. Without exception, upregulation of these cytokines is inhibited when miR-301a is deleted. These results underscore the importance of miR-301a activation of both NF-κB and Stat3 in controlling cytokine expression and promoting tumorigenesis.
Previously, miR-301a was shown to be an activator of NF-κB in epithelial-derived tumor cells [19] and macrophages [42], as well as an activator of Stat3 in T cells [21]. We dissected the role of miR-301a in tumor cells using Ras-driven MEF transformation and in immune cells using bone marrow transplantation. First, miR-301a-null transformed cells grew similar sizes as their WT counterparts when both are inoculated into the same immunodeficient host, yet the same syngeneic tumor cells (either LCC1 or B16) grew faster in WT mice than in miR-301a-null mice. Second, we replaced the bone morrow of WT mice with that from miR-301null mice or their WT littermates, and found that WT mice possessing miR-301-null bone marrow develop less-severe colon inflammation upon DSS treatment and fewer and smaller colon tumors upon AOM/DSS treatment than WT mice possessing WT bone marrow. Collectively, these data support the conclusion that miR-301a promotes tumorigenesis primarily by its effect on immune cells (and potentially other types of cells in tumor stroma) rather than by its direct effect on Rastransformed cells (Figure 8).
In summary, we have shown that miR-301a is critical for NF-κB and Stat3 activation in the tumor microenvironment in multiple mouse tumorigenesis models. Our results suggest that specific pharmacological inhibition of miR-301a in tumor-associated immune cells is an effective tool against deadly solid malignancies such as lung cancer and colon cancer. It is notable that many key components of NF-κB and Stat3 signaling are essential to embryonic viability in mice [43,44], and excessive and prolonged inhibition of NF-κB and Stat3 could be detrimental to the whole organism, owing to their roles in innate and adaptive immunity. miR-301a is expressed at extremely low levels in major tissues of adult mice [45], and except for being overexpressed in cancers and transiently expressed during embryonic development, miR-301a levels remain low in most human tissues under physiological conditions [46]. Moreover, mice without miR-301a appear to have no developmental or physiological abnormalities, which is surprising since miR-301a is such a critical regulator of two major inflammation pathways. This implicates that there is unknown functional redundancy of Nkrf and/or Pias3, as we note that mice with NKRF deletion are viable and are indistinguishable from WT mice under physiological conditions or challenged with various pathogens [47]. Nonetheless, targeting miR-301a may be superior to targeting NF-κB and Stat3 in therapeutic development against inflammation-associated cancers.

Lung tumorigenesis
Kras LA2 mice maintained in a C57BL/6J (B6) background were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Generation of miR-301a −/− mice in the C57BL/6J background was described in supplemental experimental procedures. We performed qPCR to determine the mRNA levels of SKA2 (the host gene) and miR-301a in lung and colon tissues from WT and miR-301a −/− littermates, and found that there was no difference in SKA mRNA levels, and that miR-301a was undetectable in miR-301a −/− tissues. miR-301a −/− mice were bred with Kras LA2 mice to obtain miR-301a +/ − ; Kras LA2 mice, which were intercrossed to generate miR-301a −/− ; Kras LA2 , and Kras LA2 mice. Mice were killed at the indicated experimental time or when significant tumor burden or distress was found. For survival analysis, 34 Kras LA2 mice and 28 miR-301a −/− ; Kras LA2 mice were analyzed weekly after 8 weeks of age and were killed when exhibiting shortness of breath or other signs of mortal sickness.

CAC models
CAC was induced with AOM/DSS as described [26]. Briefly, mice were injected intraperitoneally with 10 mg kg − 1 AOM (Sigma, St. Louis, MO, USA) on the first day. After 5 days, mice were fed with drinking water containing 2.5% DSS for 5 days. Animals were then given regular drinking water for 14 days, followed by two additional DSS treatment cycles. On day 140, colons were collected, and tumors were counted and measured under a dissecting microscope.

Acute colitis
Acute colitis was induced by following a standard DSSinduced inflammatory bowel disease protocol. Eight-week-old WT and miR-301a −/− male mice (n = 10 per group) were fed with drinking water containing 3.5% DSS for 5 days, followed by recovery for 5 days on normal drinking water. Mice were monitored twice weekly based on three parameters: stool consistency, fecal blood, and anal prolapse. Histopathological alterations in the colon were examined on hematoxylin and eosin-stained sections. The evaluation of colitis and severity score was performed as described [48]. Grade 0, normal colonic mucosa; Grade 1, shortening and loss of the basal one-third of the actual crypts with mild inflammation and edema in the mucosa; Grade 2, loss of the basal two-thirds of the crypts with moderate inflammation in the mucosa; Grade 3, loss of all crypts with severe inflammation in the mucosa but with the surface epithelium still remaining; and Grade 4, loss of all crypts and the surface epithelium with severe inflammation in the mucosa, muscularis propria, and submucosa.

Histology and immunohistochemistry analyses
To evaluate tumor burden and incidence, tissues and tumors were collected at the indicated dates and perfused with 10% neutral-buffered formalin. After 3 days fixation, tissues were paraffin-embedded, sectioned (5 μm), and stained with hematoxylin or eosin for morphological analyses. For immunohistochemical staining, deparaffinized and rehydrated sections were boiled in Na-citrate buffer (10 mM, pH 6.0) for 10 min for antigen retrieval. Sections were incubated with primary antibodies for 2 h at room temperature. The antibodies used in this study are listed in Supplementary Table S2. Tissue sections were developed using the Ultra Vision Detection System Anti-Rabbit (Thermo Fisher Scientific, Waltham, MA, USA). For cases in which cells per microscopic area were counted, 1500 cells were counted per unit area (n = 6) in each group to calculate the percentage of positively stained cells. For immunofluorescence staining to measure NF-κB and Stat3 in myeloid cells, antibodies against phospho-p65, phospho-Stat3, and CD11b/c were used. The CD11b/c antibodies react with macrophages, granulocytes, and dendritic cells. After rinsing with PBS, the sections were incubated with Alexa Fluor 633 goat anti-rabbit antibody (Invitrogen) for 1 h at room temperature. Sections were finally incubated with CD11b/c antibodies for 1 h, followed by 1 μg ml − 1 4′, 6-diamidino-2-phenylindole (Sigma) for 5 min. Antibodies from Abcam (Cambridge, MA, USA) (p-p65, ab28856; CD31, ab28364; CD11b/c, ab1211; IL-6, ab6672; IL-17, ab79056; CD3, ab16669; and TNFα, ab6671) and Cell Signaling (p-Stat3, no. 9145s; Ki67, no. 12202S) were used in these immunohistochemistry analyses. Slides were analyzed by confocal microscopy (Olympus, Center Valley, PA, USA). For the TUNEL assay, deparaffinized sections were incubated with 20 μg ml − 1 proteinase K for 15 min at room temperature, washed with PBS, and incubated with TUNEL reaction mixture (Roche Applied Science) for 1 h at 37°C in a humidified atmosphere. After staining with 4′, 6-diamidino-2-phenylindole, images were acquired using an Olympus IX51 microscope and analyzed using cellSens Dimension software (Olympus).

ELISA for cytokine expression
To determine cytokine secretion ex vivo, colon tissues were cut into small pieces, weighed, and incubated with Dulbecco's Modified Eagle's medium (DMEM) medium (~50 mg ml − 1 ) at 37°C for 48 h. IL-6, TNF-α, and IL-17a concentrations in culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol.

Mouse primary cell preparation
MEFs were prepared from E13.5 embryos according to an established protocol [49,50]. CD4 + T cells, CD11b + cells, and CD11c + cells were isolated from spleens using MicroBeads (Miltenyi Biotec, San Diego, CA, USA). Bone marrow-derived macrophages were prepared using a documented protocol [51]. Briefly, 8-week-old male mice were killed and the hind legs were removed. The femurs were separated and flushed with PBS using a 3-ml syringe and a 23-gauge needle. Bone marrow was passed through a pipette several times to make a single-cell suspension. Cells were washed with PBS twice before plating into a culture dish. Cells were maintained in DMEM supplemented with 10% fetal bovine serum and 10 ng ml − 1 murine granulocyte macrophage colony-stimulating factor (R&D Systems) and incubated at 37°C in 5% CO 2 .

MEF transformation, soft agar, and xenograft assay
Low-passage MEFs were transformed using retrovirus prepared with pBABE-puro SV40 LT and pWZL Blast H-Ras (Addgene, Cambridge, MA, USA). After antibiotic selection, cells were split into six-well plates at 1 × 10 5 cells per plate to evaluate cell proliferation. Cell numbers were determined using an automated cell counter (Countess, Invitrogen, Waltham, Modulation of tumorigenesis by miR-301a MA, USA). For soft agar assays, 5 × 10 3 cells were suspended in complete medium containing 0.3% Noble agar and seeded into a six-well plate containing 0.6% Noble agar in the wells. Medium was changed twice per week. After 21 days, colonies were stained with 0.005% crystal violet and counted by using a dissecting microscope. For in vivo tumor formation, 5 × 10 5 cells were injected subcutaneously into athymic nude mice (Foxn1 nu / Foxn1 nu ; Jackson Laboratory). For syngeneic xenografts, 1 × 10 5 cells were injected subcutaneously into WT or miR-301a −/− mice. Tumor size was measured every 3 days. Tumor volume was calculated by the formula of width 2 × length/2.

RNA preparation and qPCR
To measure cytokine expression, tissues or tumors were dissected from mice, and total RNA was extracted using Trizol (Invitrogen) and run on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) 2100 to ensure that the A260/ A280 ratio was in the range of 1.8-2.2 and the rRNA ratio (28S/ 18S) was 40.9. RNA was reverse transcribed with the iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). Real-time PCR was performed using the iQ SYBR Green Supermix (Bio-Rad) and CFX96 Real-Time PCR detection system (Bio-Rad). The primers (Supplementary Table S1) used for real-time PCR were designed based on the Universal Pro-beLibrary (Roche, Roche Life Science, Pleasanton, CA, USA) with ACTB as reference.

Statistical analysis
Statistical analysis was carried out using SPSS16.0 software (SPSS Inc. Chicago, IL, USA). An unpaired two-tailed Student's t-test was performed for two group comparisons where data were normally distributed; and a one-way analysis of variance test was performed for multiple group comparisons or nonparametric data. For the survival analysis, Kaplan-Meier curves were analyzed by log rank test. The statistical significance was set at a P-value o 0.05. Unless otherwise indicated, values were presented as mean ± s.d.
All experimental procedures involving animals in this study were reviewed and approved by the Institutional Animal Care and Use Committee at the University of Louisville.