Lipolysis of adipose tissue is increased in male Casp2−/− mice. Histological analyses of gWAT from (a) male and (b) female fed and fasted WT and Casp2−/− mice (×20 magnification). Bar graphs show mean adipocyte size per field of view as measured in ImageJ from >200 cells per mouse from three random fields of view (n=4–8). Metabolic gene expression in gWAT of (c) male and (d) female fed and fasted mice. Values are mean±S.D. (n=4–8). One-way ANOVA (c and d) or unpaired t-test (a and b): *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. Scale bar, 100 μm.