Table 2: Antibodies for western blot analysis

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Primary antibody Size Company Catalog No Dilution
Anti-rabbit GAPDH 37 kD GOOD HERE, Hanzhou AB-P-R 001 1 : 1000
Anti-rabbit RAMP3 16 kD Abcam ab78017 1 : 500
Anti-rabbit CALCR 56.9 kD Abcam ab11042 1 : 500
Anti-rabbit LC3 14, 16 kD CST 2775 1 : 1000
Anti-rabbit AKT 60 kD ProteinTech Group 10176-2-AP 1 : 1000
Anti-rabbit P-AKT 60 kD CST 4060P 1 : 2000
Anti-rabbit mTOR 289 kD Bioworld BS3611 1 : 800
Anti-rabbit P-mTOR 289 kD Bioworld BS4706 1 : 800
Anti-rabbit mmp3 60 kD CST 14351 1 : 1000
Anti-rabbit mmp9 92 kD Bioworld BS6893 1 : 1000
Anti-rabbit mmp13 54 kD Abcam ab39012 1 : 4000
Anti-rabbit ADAMTS4 90 kD Elabscience EAP1002 1 : 1000
Anti-rabbit ADAMTS5 102 kD Abcam ab135656 1 : 80
Anti-mouse Aggrecan 50–60 kD Abcam ab3778 1 : 100
Anti-goat colII 190 kD Santa SC-7764 1 : 500
Anti-rabbit P38 41 kD ProteinTech Group 14064-1-AP 1 : 800
Anti-rabbit P-P38 43 kD CST 4511P 1 : 1000
Anti-rabbit JNK 54, 46 kD CST 9252 1 : 1000
Anti-rabbit P-JNK 46, 54 kD CST 4668P 1 : 1000
Anti-rabbit PI3K 85 kD CST 4257P 1 : 1000
Anti-goat P-PI3K 85 kD Santa SC-12929 1 : 500