Figure 8

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 8

Effects of IAPP on the expression of PI3K/AKT-mTOR and p38/JNK MAPK signaling. (a) Western blot analysis revealed the phosphorylated and unphosphorylated protein levels of PI3K/AKT-mTOR signaling pathway. (b) Western blot analysis revealed the phosphorylated and unphosphorylated protein levels of P38 and JNK. (ce) Statistical analysis of the ratio of P-PI3k/PI3K (c), p-AKT/AKT (d), and p-mTOR/mTOR (e) in different groups, including NC, pLV-veh, pLV-IAPP, Ctrl siIAPP, pLV-siIAPP, and Pramlintide. (fh). Statistical analysis of the ratio of P-P38/P38 (f), p-JNK1/JNK1(g), p-JNK2/JNK2 (h) in different groups. Data are representative of three independent experiments, and P-values are shown: *P<0.05; **P<0.01.