Figure 7

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 7

Apoptosis and autophage Assay in NP cells induced by IAPP. (a) TUNEL assay to identify apoptotic cells (red) in NP cells transfected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2- Puro-IAPP, or treated with pramlintide. (Scale bar magnification: ×400) (c). Statistical analysis showing results of apoptosis incidence. (b) EM analysis showed autophagosome formation in NP cells of different groups. Scale bar: 1 μm. (d) Statistical diagram showing autophagosome counts. Values represent the means±S.D. *P<0.05, **P<0.01, compared with NC.