Figure 6

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 6

ROS content and apoptosis induced by IAPP were determined by Flow Cytometry. (a) Intracellular ROS generation was determined by measuring the fluorescence intensity using flow cytometry in NP cells infected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2- Puro-IAPP, or treated with pramlintide. (b) Statistical analysis showing results of cell fluorescence for DHE. (c) Flow cytometric measurement for apoptosis incidence. (d). Statistical analysis showing TUNEL-positive rate. For quantitative evaluations, data were shown as means±S.D. *P<0.05, **P<0.01, compared with the NC.