Figure 5

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 5

Effects of IAPP on apoptotic and autophagic gene expression in NP cells. NP cells were transfected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2-Puro-IAPP, or treated with pramlintide. (ac) RT- PCR analysis was used to detect the mRNA expression of apoptotic genes, including caspase-3 (a), Bcl-2 (b), and Bax (c). (df) RT- PCR analysis was used to detect the mRNA expression of autophagic genes, including BECLIN1 (d), ATG5 (e), and ATG7 (f). (g) Protein expression of LC-3 in different groups of NP cells was determined by western blotting. (h and i) Statistical analysis showing the relative expression of LC3-2 (h) protein and the ratio of LC3-2/LC3-1 (i). (j) Representative images of caspase-3 protein expression detected by immunofluorescence staining. Data were shown as means±S.D. *P<0.05, **P<0.01, compared with the NC. (Scale bar magnification: ×400).