Figure 4

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 4

Effects of IAPP on the expression ECM catabolic genes in human NP cells. The cells were transfected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2-Puro-IAPP, or treated with pramlintide. (a) Protein expressions of MMP-3, MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 were determined by western blotting. (bh) Statistical analysis showing the relative expression of MMP-3 (b), MMP-9 (c), MMP-13 (d), ADAMTS-4 (e), ADAMTS-5 (f), Aggrecan (g), and Col2 (h) proteins. Values represent the means±S.D. *P<0.05, **P<0.01, compared with NC.