Figure 3

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 3

The effects of IAPP on ECM anabolic gene expression and pro-inflammatory factor secretion in NP cells. Immunocytochemistry assay revealed IAPP protein expression in NP cells infected with pLVX-mCMV-Puro, pLVX-mCMV-IAPP, PLVX-ShRNA2-Puro, pLVX-ShRNA2-Puro-IAPP, or treated with pramlintide. (a) Immunocytochemistry assay revealed IAPP protein expression in NP cells after transfection. (b) RT-PCR showed the expression of IAPP in NP cells in different groups. (c) MTT assay showed higher cell viability resulting from the upregulation of IAPP. (d) Glucose uptake measurement. E-H. RT- PCR showed gene expression of aggrecan (e), Col2 (f), and downregulation of TNF-α (g) and IL-1 (h) in different groups. (i and j). ELISA analysis showed protein expression of IL-1 (i) and TNF-α (j) in human NP cells of different groups. *P<0.05, **P<0.01, compared with NC.