Figure 1

From: IAPP modulates cellular autophagy, apoptosis, and extracellular matrix metabolism in human intervertebral disc cells

Figure 1

Detection of IAPP expression in human IVD tissue. (a) Representative images of IAPP protein in human IVD tissue detected by immunofluorescence staining. (b) IAPP, AG, BG, and Col mRNA levels in human IVD tissue samples were determined by RT-qPCR. (c) Statistical analysis showing the relative expression of IAPP mRNA in IVD tissue with varying degrees of degeneration. (dg) Statistical analysis showing the relative expression of AG (d), BG (e), Col2A1 (f), and Col1A2 (g) mRNAs. Data were shown as means±S.D. *P<0.05, **P<0.01, compared with group 2 (non-degenerated IVD). Grades 3, 4, and 5 signify mild, moderate, and severe degeneration, respectively. (Alcian blue staining ×100; IHC ×200).