Figure 6

From: Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling

Figure 6

SNX2 depletion enhances Met signaling. (a) HeLa cells were transfected two times at a 24 h interval with control (siCTL) or a SNX2 small interfering RNA (siRNA). At 56 h, cells were serum-starved for 16 h and then stimulated with HGF as indicated. (b) HeLa cells were treated with STS (0.5 μM) for the indicated period of time. (c) Serum-starved HeLa cells were treated with UV (100 J/m2, 16 h) and HGF (100 ng/ml, 15 min). Proteins were analyzed by immunoblotting using the indicated antibodies. Closed and open arrowheads indicate full-length and cleaved fragments, respectively. Actin was used as a loading control. Asterisks indicate nonspecific proteins recognized by the antibodies.