Figure 1

From: Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling

Figure 1

The retromer-associated proteins SNX1 and SNX2 are cleaved during apoptosis. (a and b) HeLa cells were left untreated (−) or treated for 16 h with the following conditions: UV (254 nm wavelength, 100 J/m2); STS (0.5 μM); TRAIL (100 ng/ml) in serum-free medium; cycloheximide (CHX, 10 μg/ml); tumor necrosis factor (TNF) (50 ng/ml); and Z-VAD-fmk (10 μM). Proteins were analyzed by immunoblotting using the indicated antibodies. In (b), two different immunoblotting protocols were used to reveal cleaved SNX1 fragments. (c) Schematic representations of SNX1 and SNX2 proteins. The limits of domains are indicated by numbers; the epitopes recognized by the antibodies used in this study are indicated. Closed and open arrowheads indicate full-length and cleaved fragments, respectively. Actin was used as a loading control. Asterisks indicate nonspecific proteins recognized by the antibodies.