Figure 4 | Cell Death & Disease

Figure 4

From: Maml1 acts cooperatively with Gli proteins to regulate sonic hedgehog signaling pathway

Figure 4

Maml1 loss of function determines an impaired Shh signaling pathway. (a) Immunoblot analysis of WCE from Maml1-deficient (Maml1−/−) and control (Maml1+/+) MEFs, using the indicated antibody. (b) Optical densitometry (OD) analysis of Maml1, Gli1 and Gli2 protein expression evaluated by immunoblotting, related to panel (a). (c) Representative qRT-PCR analysis of Hh target genes in Maml1−/− MEFs compared with control upon SAG treatment for 48 h. The data are presented as fold of activation respect to DMSO. (d) qRT-PCR analysis of Maml1 mRNAs and Shh target genes in Ptch1−/− MEF cells transfected with Maml1 siRNA or control siRNA (scr) for 48 h. (e) Trypan blue cell counting to determine the rate of proliferation and the number of viable cells in Ptch1−/− MEFs after transfection with Maml1 siRNA for 48 h, compared with scramble (scr). (f) MTT cell proliferation assay in Ptch1−/− MEF cells Maml1-silenced compared with control. The data are presented as fold of reduction respect to scramble. Data represent mean±S.D. **P≤0.01; ***P≤0.001; ****P≤0.0001

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