Protein kinase A activation by the anti-cancer drugs ABT-737 and thymoquinone is caspase-3-dependent and correlates with platelet inhibition and apoptosis

Chemotherapy-induced thrombocytopenia is a common bleeding risk in cancer patients and limits chemotherapy dose and frequency. Recent data from mouse and human platelets revealed that activation of protein kinase A/G (PKA/PKG) not only inhibited thrombin/convulxin-induced platelet activation but also prevented the platelet pro-coagulant state. Here we investigated whether or not PKA/PKG activation could attenuate caspase-dependent apoptosis induced by the anti-cancer drugs ABT-737 (the precursor of navitoclax) and thymoquinone (TQ), thereby potentially limiting chemotherapy-induced thrombocytopenia. This is particularly relevant as activation of cyclic nucleotide signalling in combination chemotherapy is an emerging strategy in cancer treatment. However, PKA/PKG-activation, as monitored by phosphorylation of Vasodilator-stimulated phosphoprotein (VASP), did not block caspase-3-dependent platelet apoptosis induced by the compounds. In contrast, both substances induced PKA activation themselves and PKA activation correlated with platelet inhibition and apoptosis. Surprisingly, ABT-737- and TQ-induced VASP-phosphorylation was independent of cAMP levels and neither cyclases nor phosphatases were affected by the drugs. In contrast, however, ABT-737- and TQ-induced PKA activation was blocked by caspase-3 inhibitors. In conclusion, we show that ABT-737 and TQ activate PKA in a caspase-3-dependent manner, which correlates with platelet inhibition and apoptosis and therefore potentially contributes to the bleeding risk in chemotherapy patients.

release, shape change, adhesion, and aggregation. 16,17 In nucleated cells, both cAMP and cGMP can induce pro-and anti-apoptotic effects. [18][19][20][21] In our study, we used two anti-cancer substances, ABT-737 and thymoquinone (TQ) with different mechanisms of inducing apoptosis and compared them with Thr/Cvx triggered apoptotic-like events in platelets. ABT-737, a precursor of the oral derivate ABT-263 (navitoclax), is a potent mimetic of Bcl-2 homology 3 domain (BH3)-only proteins (including the Bcl-2 interacting mediator of cell death (Bim), BH3 interacting domain death agonist (Bid) and other proteins which are important in binding and neutralizing anti-apoptotic Bcl-2 family proteins). 22,23 TQ is an active component of Nigella sativa and acts as a multiple target modulator in cancer control via p53, 24 nuclear factor-kappaB, 25 protein kinase B suppression, 26 caspase activation, 27 and activation of tumour suppressor factor as well as peroxisome proliferator-activated receptor. 28 In platelets, TQ induces apoptosis by increase of cytosolic calcium concentration, phosphoinositide 3-kinase and caspase-3 activation, ceramide formation, and mitochondrial depolarization. 29 The mechanisms of Thr/Cvx-induced platelet activation and pro-coagulant activity are well characterised 13,[30][31][32] and we used this model as a positive control to compare PKA/PKG effects on platelet apoptosis induced by other stimuli.
Here, we show that activation of PKA/PKG did not prevent ABT-737-and TQ-induced platelet apoptosis. In contrast, both ABT-737 and TQ activated PKA by cAMP-independent but caspase-3-dependent mechanisms and strongly inhibited thrombin-induced platelet activation.

Results
ABT-737and TQ induce platelet apoptosis, whereas Thr/Cvx induces pro-coagulant platelets. First, optimal incubation times and concentrations of the compounds required to induce apoptotic or pro-coagulant platelets were established (data not shown). In all experiments, platelets were treated with ABT-737 (1 μM) or TQ (40 μM) for 10 and 60 min or with a combination of Thr (0.005 U/ml) and Cvx (5 ng/ml) for 2 min. All compounds decreased ΔΨ m to approximately 30% compared to untreated platelets (Figure 1a). ABT-737 induced strongest PS exposure (13 ± 4-fold increase compared to control), whereas TQ (5.5 ± 0.9) and Thr/Cvx (7.1 ± 1.0) induced PS exposure was significantly less pronounced ( Figure 1b). Both TQ and ABT, but not Thr/Cvx evoked significant caspase-3 activation (Figure 1c). These experiments confirmed that under the tested conditions Thr/Cvx treatment caused caspase-3-independent formation of procoagulant platelets, whereas ABT-737 and TQ induced caspase-3-dependent platelet apoptosis.
PKA/PKG activation inhibits Thr/Cvx-triggered procoagulant state, but not ABT-737-or TQ-induced platelet apoptosis. Next, we investigated the impact of PKA or PKG activation on Thr/Cvx-triggered pro-coagulant state and on ABT-737-or TQ-induced platelet apoptosis. Kinase activities were monitored using the well-established and highly sensitive detection of VASP S239-phosphorylation (P-VASP S239 ), which has been used in numerous studies. 16,33,34 In platelets, both PKA and PKG equally phosphorylate VASP at Ser239. Thr/Cvx stimulation alone did not induce VASP phosphorylation. However, activation of PKA by forskolin or PKG by the nitric oxide (NO) donor DEA-NO triggered strong VASP phosphorylation ( Figure 2a) and significantly reduced the Thr/Cvx-induced platelet activation as revealed by a Figure 1 Platelet apoptotic events induced by ABT-737, TQ, and Thr/Cvx. Washed human platelets (1 × 10 7 /ml) were incubated with ABT-737 (1 μM, 10 min), TQ (40 μM, 60 min), or a combination of thrombin (Thr, 0.005 U/ml) and convulxin (Cvx, 5 ng/ml) for 2 min. (a-c) FACS analyses of mitochondrial membrane potential (ΔΨ m ) (a), PS exposure (Annexin V binding (b), and caspase-3 activation (c) are shown. Data are presented as fold changes of mean fluorescence intensity (MFI) compared to control (considered as 1) and as mean ± S.E.M., n = 5; *Po0.05 compared to control, # Po0.05 compared to ABT-737, ‡ Po0.05 compared to Thr/Cvx ABT-737 and TQ induce VASP phosphorylation in platelets. Surprisingly, we found that stimulation of platelets with ABT-737 or TQ induced strong VASP phosphorylation.
The magnitude of VASP phosphorylation was comparable to the activation of PKG by DEA-NO or PKA by forskolin stimulation (Figures 4a-c), indicating a possible activation of one or both of the kinases by these compounds. Indeed, coincubation with forskolin or DEA-NO did not further increase VASP phosphorylation levels ( Figure 4a). We also investigated the kinetics of TQ/ABT-737-induced VASP phosphorylation (Figures 4b and c). TQ-and ABT-737-induced VASP phosphorylation was slow compared to the DEA-NO-and forskolin-induced VASP phosphorylation, which was already maximal after 2 min at the latest (compare Figure 2a). ABT-737-induced VASP phosphorylation was still low at 10 min and peaked at 60 min of incubation. TQ-dependent VASP phosphorylation reached half-maximal levels after 10 min and a sustained and maximal phosphorylation was ABT-737-and TQ-induced inhibition of platelet activation correlates with VASP phosphorylation. ABT-737 was shown to prevent platelet activation despite PS exposure and thrombin generation. 6 First, we confirmed that ABT-737 and TQ themselves did not induce platelet activation (integrin activation assessed by PAC-1 binding and P-selectin exposure) during 10 and 60 min incubation (data not shown). ABT-737 induced PS exposure (Annexin V binding) and abolished thrombin-triggered integrin activation (PAC-1 binding) after 60 min of treatment, but did not affect the activation at 10 min (Figures 5a and b). Therefore, the inhibition of platelet activation and increase of PS-exposure directly correlated with the time course of VASP phosphorylation, which was still low after 10 min but maximal after 60 min of ABT-737 incubation (Figure 5c). In case of TQ-treatment, PS-exposure and inhibition of integrin activation were already . Again, inhibition of platelet activation and increase of PS-exposure directly correlated with TQ-induced VASP phosphorylation, which followed the same kinetics ( Figure 5f). Besides kinase activation, the observed inhibition of platelet activation by ABT-737 or TQ may also be related to reduced platelet viability and/or integrin surface expression. We used the vital dye calcein-AM, a fluorogenic substrate of intracellular esterases, for the reliable assessment of platelet viability 35,36 and αIIbβ3 integrin/CD41-specific antibodies to determine the surface expression of the glycoprotein by FACS. After 10 min of incubation with ABT-737 or TQ, platelet viability and CD41 surface expression were statistically undistinguishable from control values (Figures 6a and b). This is especially noteworthy for the TQ-treatment, because inhibition of platelet activation by TQ was nearly maximal under this condition ( Figure 5e). After 60 min of ABT-737 or TQ treatment, reduced esterase activity was observed in 23% and 45% of the platelets, respectively. However, the percentage of calceinnegative/dead cells was still low, 6.8% and 6.7%, respectively, and did not significantly differ from the control group (4.7%; Figure 6c). Importantly, CD41 surface expression was again completely unaffected by ABT-737 or TQ incubation. Taken together, the data indicate that ABT-737 or TQ-mediated platelet inhibition of thrombin-induced activation (more than 70%, Figures 5b and e) is not directly related to a reduced platelet viability or integrin αIIbβ3 surface expression.
ABT-737 and TQ do not increase cAMP or cGMP levels in human platelets. PKA and PKG are activated by elevated cAMP and cGMP levels, respectively. Therefore, we measured whether ABT-737 and TQ elevate cyclic nucleotides by activating cyclases or by inhibiting cyclic nucleotide hydrolysing phosphodiesterase (PDE) in platelets. DEA-NO and forskolin directly stimulate the GC and AC, respectively, and hence were used as positive controls while the non-specific PDE inhibitor IBMX was used as a negative control. Unexpectedly, under conditions which strongly induced ABT-737 and TQ-dependent VASP phosphorylation ( Figure 5), we did not observe any increase in cyclic nucleotide concentrations (Figures 7a and b). To confirm that ABT-737 and TQ did not activate AC or GC we used AC inhibitor SQ22536 and GC inhibitor ODQ. Pre-incubation of platelets with SQ22536 and ODQ completely blocked forskolin and DEA-NO-induced VASP phosphorylation, but had no effect on ABT-737 or TQ-induced VASP phosphorylation (Figure 7c).
ABT-737 and TQ do not activate PP1 and PP2 phosphatases. VASP phosphorylation is dynamically regulated by the kinase activity of PKA/PKG on the one hand and the phosphatase activity of protein phosphatase 1A (PP1A) and  37 Because increased VASP phosphorylation by ABT-737 and TQ could be an indirect consequence of Ser/Thr phosphatase inhibition, we tested the impact of the compounds on Ser/Thr phosphatase activity. For this, we used two different approaches. In the first experiment, platelets were incubated with ABT-737, TQ, and Calyculin A (CA), a well-established Ser/Thr phosphatase inhibitor, and platelet lysates were analysed by Western blot with phospho-Ser/Thr specific antibodies. While all three compounds induced strong VASP phosphorylation, only incubation with CA resulted in numerous additional phospho Ser/Thr proteins of different molecular weight (Figure 7d). After ABT-737 or TQ treatment, however, only few Ser/Thr phosphorylated proteins emerged on the blot, indicating no strong effect of both compounds on phosphatases activity in intact cells. To confirm this with an independent approach, we directly tested whether ABT-737 or TQ could inhibit the phosphatase activity of PP1A and PP2A in vitro. In this assay, phosphatase activity was measured by free phosphate concentration released from a phosphatase-specific substrate. However, neither ABT-737 nor TQ did affect Ser/Thr phosphatase activity of PP1A or PP2A (Figure 7e).
ABT-737 and TQ activate PKA by a caspase-3 dependent mechanism. So far, our data demonstrate that ABT-737 and TQ induce VASP phosphorylation independent of cyclic nucleotides and also independent of phosphatase activity. To distinguish between PKA and PKG activation, we studied ABT-737/TQ-induced VASP phosphorylation in platelets after pre-incubation with membrane permeable PKA-(H89 and Rp-8-Br-cAMPS) and PKG-inhibitors (Rp-8-Br-PET-cGMPS). 38 Pre-incubation of platelets with H89 and Rp-8-Br-cAMPS strongly inhibited ABT-737-, TQ-, and forskolininduced VASP phosphorylation, while Rp-8-Br-PET-cGMPS only inhibited DEA-NO-induced VASP phosphorylation (Figure 8a). The data clearly indicate that VASP phosphorylation is mediated by PKA, but not PKG activation. PKA is comprised of two catalytic (C) subunits that bind to a regulatory subunit dimer to form the inactive holoenzyme. For PKA activation, the C-subunits are released from the regulatory subunits. To test whether ABT-737 or TQ can directly activate PKA we incubated both compounds with PKA holoenzyme in vitro and studied release of the catalytic subunits. Incubation of PKA with cAMP resulted in strong release of the free C-subunit. In contrast, neither ABT-737 nor TQ increased free C-subunit levels as observed in native gels (Figure 8b), indicating that both compounds are not direct PKA activators in vitro. Because ABT-737 and TQ activated caspases, we tested whether PKA activation might be caspase-dependent. Pre-incubation of platelets with pancaspase (z-VAD-fmk, data not shown) and caspase-3 specific (z-DEVD-fmk) inhibitors completely blocked Figure 6 Impact of ABT-737-and TQ-treatment on platelet viability and integrin αIIbβ3 surface expression. Washed human platelets (1 × 10 8 /ml) were treated with ABT-737 (1 μM) or TQ (40 μM) for the indicated times and analysed by FACS for viability (calcein-fluorescence) and integrin αIIbβ3 surface expression (CD41). All platelets were separated into three gates: gate A represents more than 93% calceinand CD41-positive platelets in control samples, gate C corresponds to unstained platelets (calcein-negative/dead cells; or CD-41-negative platelets; compare (a)) and gate B represents platelets with intermediate calcein-or CD41 fluorescence. Distribution of platelets in each gate was calculated as a percent of all analysed (50 000) events taken as 100%. Data are presented as mean ± S.E.M., n = 4. (a-c) represent a set of original FACS data representative of four independent experiments ABT-737 and TQ activate PKA in platelets N Rukoyatkina et al ABT-737-induced VASP phosphorylation, whereas TQinduced phosphorylation was only partly inhibited ( Figure 8c). We also tested whether active caspase-3 can activate PKA in vitro and incubated PKA holoenzyme with active caspase-3. However, active caspase-3 did not release C-subunit from the holoenzyme (Figure 8d and supplementary Figure 1), indicating that caspase-3 cannot directly activate PKA in vitro.
Together, these data suggested that ABT-737-and TQinduced VASP phosphorylation in intact platelets could be, at least partly, mediated by cAMP-independent and caspase-3dependent mechanisms of PKA activation.

Discussion
Thrombocytopenia and bleeding disorders are the primary, dose-limiting adverse effects of anticancer therapy. Recent data from mouse and human platelets revealed that activation of PKA/PKG not only inhibited Thr/Cvx-induced platelet activation but also its pro-coagulant state. 13 In the present study, we tested whether or not PKA/PKG activation could inhibit caspase-dependent apoptosis induced by ABT-737 (navitoclax) or thymoquinone (TQ), thereby limiting chemotherapy-induced thrombocytopenia. This is especially important given that activation of cyclic nucleotide signalling in combination chemotherapy is an emerging strategy in cancer treatment. [39][40][41] However, PKA/PKG-activation did not block caspase-3-dependent platelet apoptosis induced by the compounds (Figures 3b and c). In contrast, both substances induced PKA activation themselves and PKA activation ( Figure 4) correlated with platelet inhibition and apoptosis ( Figure 5). We tested whether ABT-737-and TQ-induced PKA activation is mainly responsible for the inhibition of thrombininduced platelet activation, or if this effect may be a In vitro phosphatase assay: the PP1A/PP2A-driven release of free phosphate from a defined phosphopeptide after 15 min incubation (corresponding to 50% phosphate release of maximal phosphate release measured after 1 h incubation with PP1A or PP2A) was measured in the presence and absence (ø) of TQ or ABT-737, using a colorimetric reaction at 600 nm as described in the method part. Data are presented as optical density (OD) at 600 nm (mean ± S.E.M., n = 3; n.s. no significant difference compared to phosphatase reactions without TQ/ABT-737 supplementation) ABT-737 and TQ activate PKA in platelets N Rukoyatkina et al consequence of the decreased platelet viability or integrin αIIbβ3 surface expression. However, neither treatment with TQ or ABT-737 significantly changed the number of dead (calcein-negative) platelets or integrin αIIbβ3 surface expression in platelets compared to control cells ( Figure 6) under conditions that showed maximal platelet inhibition and PKA activation (VASP phosphorylation, Figures 5e and f).
The PKA holoenzyme in its inactive form is a tetramer consisting of two regulatory subunits (R) and two catalytic subunits (C). Both R subunits contain two binding sites for cAMP. Upon binding of cAMP the two C subunits are released and activated to phosphorylate their substrates. In addition to this 'classical' cAMP-dependent PKA activation, at least three other cAMP-independent mechanisms are described: binding of PKA-C-subunit to IkB in NFkB-IkB complex, 42,43 interaction of p90 ribosomal S6 kinase (RSK1) with PKARIα, 44 and transforming growth factor beta-induced Smad3/Smad4 complex formation. 45 Furthermore, some small molecules can Proteins were resolved on a 10% native acrylamide gels and processed for western blotting with PKAC antibodies. (c) Washed human platelets were stimulated as indicated without (ø) or with preincubation with caspase-3 inhibitor (zDEVDfmk, 100 μM, 5 min). Samples were analysed by western blot for VASP Ser239 phosphorylation. For the bar graphs, immunoblots of four independent experiments were scanned and quantified with ImageJ. The intensity of the VASP Ser239 signal was normalized to the actin signal, which was designated as 1 in control samples. Results are means ± S.E.M., n = 4, + significant differences (Po0.05) compared to control samples; * significant differences (Po0.05) compared to ABT-737 or TQ samples without caspase-3 inhibitor. (d) PKA II holoenzyme was incubated with active caspase-3 for the indicated times. Proteins were resolved on a 10% native acrylamide gels and processed for Western blotting with PKAC antibodies. Presented data are representative of three independent experiments ABT-737 and TQ activate PKA in platelets N Rukoyatkina et al cAMP-independently activate PKA. 46,47 However, molecular mechanisms, how these substances directly activate PKA are not known. In 1987, VASP was discovered and characterized as the major PKA/PKG substrate in platelets. 33,48 Three decades later, measurement of VASP phosphorylation status has become a highly sensitive and reliable assay, which is used in numerous studies to assess PKA/PKG-dependent effects in cardiovascular cells and which is widely used in clinical diagnosis of platelet reactivity. 16,[49][50][51][52][53] In our study, we used VASP phosphorylation as a marker of ABT-737-or TQinduced PKA activation in platelets. Interestingly, VASP phosphorylation correlated well with platelet inhibition and apoptosis. Whether or not VASP phosphorylation plays a functional role in caspase-dependent platelet apoptosis, remains unknown. In apoptotic platelets, we did not observe apparent VASP cleavage, indicating that VASP itself is not a prominent substrate for caspase-3. However, there is at least circumstantial evidence for a role of VASP in caspase-3mediated membrane blebbing, a hallmark of apoptotic events. Cleavage of the plasma membrane-associated spectrins by caspase-3 leads to membrane blebbing, the formation of apoptotic bodies, and irreversible cell death. In endothelial cells, we have previously shown that VASP binding to αIIspectrin attenuates αII-spectrin cleavage in apoptotic cells and that PKA-induced VASP phosphorylation regulates this process. 54,55 Whether a similar mechanism exists in platelets is unclear. However, given that spectrin disruption destabilizes pro-platelets, causing blebbing and swelling, 56 it is tempting to speculate that VASP phosphorylation may be of functional importance for platelet apoptosis.
In our platelet experiments, ABT-737-and TQ-induced PKA activation was, at least partially, blocked by caspase-3 inhibitors (Figure 8c). PKA has been implicated in regulating caspase activity, 57,58 but there have been no reports of a reverse role for caspases in PKA activation. In nucleated cells, caspase-3 activation was shown to increase phosphatase PP2A activity by cleavage of the regulatory subunit. 59 We tested whether or not a similar mechanism may contribute to PKA activation. In our in vitro experiments, however, active caspase-3 in combination with ABT-737 or TQ failed to activate PKA, indicating that additional intracellular signalling pathways are required for PKA activation after treatment with the compounds.
A role of cAMP signalling in regulation of apoptosis is known for decades. However, cAMP can either stimulate or inhibit apoptosis in a cell type-dependent manner and the underlying mechanisms remain elusive. In nucleated cells, exchange protein activated by cAMP (Epac) is a second effector of cAMP-signalling that seems to mediate anti-apoptotic effects. 20 However, these cAMP effects are unlikely to play a role in platelets because Epac is not expressed in platelets 60 and there was no increase of cAMP levels in ABT-737-or TQtreated platelets. On the other hand, substantial evidence indicates that pro-apoptotic events of cAMP in nucleated cells are, at least in part, mediated by the PKA-dependent phosphorylation of protein targets. 61,62 Given that the effects of ABT-737, TQ, and PKA on nucleated cell apoptosis partially involve regulation of gene expression, it is difficult to compare these findings to the situation in platelets. For example, in Jurkat T-cells, TQ downregulated the expression of phosphodiesterases PDE1, PDE3, and PDE4 during 24 h of incubation, which increased cGMP levels and reduced cAMP concentrations. 63 In platelets, we did not see any effect of TQ or ABT-737 on cyclic nucleotide levels. All these data indicate that regulation of cyclic nucleotides as well as PKA/PKG activity by ABT-737 and TQ is cell type dependent and involves different molecular mechanisms. Whether longterm PKA/PKG activation can influence platelet apoptosis and how ABT-737 or TQ cAMP-independently activate PKA is not clear and should be addressed in a separate study.
In summary, we showed that elevation of cyclic nucleotides does not inhibit apoptosis induced by ABT-737 and TQ. In contrast, both anti-cancer drugs cAMP-independently activated PKA themselves, thereby inhibiting platelet activation. Therefore, our study provides novel insights underlying the high bleeding risk in chemotherapy patients.
Human platelet preparation. Human platelets were prepared and used as previously reported. 43,65 Blood was obtained from healthy volunteers according to our institutional guidelines and the Declaration of Helsinki. Our studies with human platelets were approved and reconfirmed by the local ethics committee of the University clinic of Wuerzburg (Studies No. 67/92 and 114/04). FACS analysis. FACS analysis was performed using a Becton Dickinson FACS Calibur with CELLQuest software, version 3.1f. For the detection of surface PS, αIIbβ3 integrins surface expression, or activated αIIbβ3 integrins, washed platelets (50 μl) were labelled with Annexin V-PE or PAC-1-FITC for 10 min at RT after stimulation. The platelets were then diluted with Annexin V-binding solution (140 mM NaCl, 10 mM HEPES, and 2.5 mM CaCl 2 ) for Annexin V or PBS for PAC-1, or CD41 and immediately analysed by flow cytometry. Caspase-3 activity was measured by rabbit anti-active caspase-3-PE antibody according to the manufacturer's instruction.
Analysis of platelet viability by calcein-AM staining and αIIbβ3 integrins surface expression. For FACS analysis of platelet viability, platelets were loaded with 0.1 μM calcein-AM for 30 min, incubated without (control) or with ABT-737 or TQ at 37°C for indicated time and then analysed for intracellular esterase activity that liberates green fluorescence calcein from the acetoxymethyl (AM) ester group leading to the accumulation of the dye within viable cells. 35 Platelets were divided into three gates with different fluorescence intensity. Gate A contains calcein positive platelets, gate B contains platelets with reduced calcein fluorescence and gate C represents calcein-negative platelets corresponding to unstained cells. αIIbβ3 integrins surface expression was analysed similarly by CD41-specific antibodies directed against the αIIb chain. Distribution of platelets in each gate was calculated as percent of all analysed (50 000) events taken as 100%.
Analysis of mitochondrial membrane potential. Washed platelets (10 7 platelets/ml) were stimulated and then incubated with JC1 dye for 10 min at RT. Mitochondrial membrane potential (ΔΨ m ) was analysed by FACS. Green ABT-737 and TQ activate PKA in platelets N Rukoyatkina et al fluorescence (FL1) and red fluorescence (FL2) were measured in logarithmic scales using voltage settings of 710 or 588 respectively. Compensation settings were performed using FITC-and PE-labelled beads (BD-Bioscience). JC1 is sensitive to ΔΨ m and the ratio of fluorescence in FL2 to FL1 corresponds to changes of ΔΨ m .
Western blot analysis. For western blot analysis, washed platelets (3 × 10 8 platelets/ml) were stimulated with indicated (time/concentration) compounds and then lysed with Laemmli sample buffer. Western blots were performed as described. 65 Determination of platelet cGMP and cAMP concentration. cGMP and cAMP content were determined in washed human platelets by a competitive immunoassay (Cayman Chemical, Hamburg, Germany) as per the manufacturer's protocol.
PP1A/PP2 in vitro phosphatase activity assay. Phosphatase (PP1A/ PP2) assay was performed with a colorimetric assay following the manufacturer's instructions (Biozol, Eching, Germany). Phosphatases were incubated with ABT-737 and TQ and the reaction was started by 200 μM threonine phosphopeptide. After 15 min, reaction was stopped by Biomol Green (ENZO Life Sciences). After 30 min at RT, colour intensity was measured with absorbance at 600 nM in Wallac Victor (Perkin Elmer, Hamburg, Germany).
PKA dissociation by ABT-737 and TQ (native gels). PKA II holoenzyme (0.1 μM, 150 ng) was incubated in phosphorylation buffer (10 mM HEPES pH 7.4, 5 mM MgCl 2 , 1 mM EDTA, 0.2 mM dithiothreitol) in the absence (control) or presence of 1 μM cAMP, 10 μM ABT-737 or 10 μM TQ. Reaction was started by the addition of 100 μM ATP and stopped after 30 min by the addition of 2 × sample buffer (62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 1% bromophenol blue) without heating. Probes were loaded on a 10% acrylamide gel without SDS. Anode and cathode buffers were prepared and used as described. 66 C-subunit of PKA was visualized by Western blotting.
In vitro caspase-3 activity. Active caspase-3 (2U) with an activity of 2 nM substrate per hour at 37°C was incubated with PKA II holoenzyme (0.1 μM, 150 ng) in the buffer supplied by manufacturer. cAMP (1 μM) was used as a positive control. Reaction was stopped after indicated times by the addition of 2 × sample buffer (62.5 mM Tris-HCl, pH 6.8, 25% glycerol, 1% bromophenol blue) without heating. Probes were loaded on a 10% acrylamide gel without SDS and processed for western blotting with PKA C-subunit antibody. Caspase-3 activity was measured colorimetrically at 405 nm by degradation of the specific substrate (Ac-DEVD-pNA) at indicated times.
Data analysis. All experiments were performed at least four times, and the data were expressed as means ± S.E.M. Differences between groups were analysed by ANOVA followed by Bonferroni's test and Student t-test was used when appropriate. Po0.05 was considered statistically significant.