TAK1 regulates resident macrophages by protecting lysosomal integrity

Hematopoietic cell survival and death is critical for development of a functional immune system. Here, we report that a protein kinase, TAK1, is selectively required for resident macrophage integrity during embryogenesis. Hematopoietic lineage-specific deletion of Tak1 gene (Tak1HKO) caused accumulation of cellular debris in the thymus in perinatal mice. Although no overt alteration in thymocytes and blood myeloid populations was observed in Tak1HKO mice, we found that thymic and lung macrophages were diminished. In the in vitro setting, Tak1 deficiency caused profound disruption of lysosomes and killed bone marrow-derived macrophages (BMDMs) without any exogenous stressors. Inhibition of the lysosomal protease, cathepsin B, partially blocked Tak1-deficient BMDM death, suggesting that leakage of the lysosomal contents is in part the cause of cell death. To identify the trigger of this cell death, we examined involvement of TNF and Toll-like receptor pathways. Among them, we found that deletion of Tnfr1 partially rescued cell death. Finally, we show that Tnfr1 deletion partially restored thymic and lung macrophages in vivo. These results suggest that autocrine and potentially paracrine TNF kills Tak1-deficient macrophages during development. Our results reveal that TAK1 signaling maintains proper macrophage populations through protecting lysosomal integrity.

Hematopoietic cell survival and death is critical for development of a functional immune system. Here, we report that a protein kinase, TAK1, is selectively required for resident macrophage integrity during embryogenesis. Hematopoietic lineage-specific deletion of Tak1 gene (Tak1 HKO ) caused accumulation of cellular debris in the thymus in perinatal mice. Although no overt alteration in thymocytes and blood myeloid populations was observed in Tak1 HKO mice, we found that thymic and lung macrophages were diminished. In the in vitro setting, Tak1 deficiency caused profound disruption of lysosomes and killed bone marrow-derived macrophages (BMDMs) without any exogenous stressors. Inhibition of the lysosomal protease, cathepsin B, partially blocked Tak1-deficient BMDM death, suggesting that leakage of the lysosomal contents is in part the cause of cell death. To identify the trigger of this cell death, we examined involvement of TNF and Toll-like receptor pathways. Among them, we found that deletion of Tnfr1 partially rescued cell death. Finally, we show that Tnfr1 deletion partially restored thymic and lung macrophages in vivo. These results suggest that autocrine and potentially paracrine TNF kills Tak1-deficient macrophages during development. Our results reveal that TAK1 signaling maintains proper macrophage populations through protecting lysosomal integrity. Macrophages possess unique functional roles that are dependent on the microenvironment where they reside. 1 During early embryogenesis, progenitors of macrophages migrate to several different sites in the embryo, uniquely differentiate, and colonize as tissue-resident macrophages, such as microglia, thymic macrophages and pulmonary macrophages. 2 When invasion of pathogenic microorganisms occurs, tissue-resident macrophages along with recruited circulating monocytes provoke inflammatory responses. In addition to their role as responders to insult, tissue-resident macrophages are also critical for tissue integrity in the steady state. Because tissues constantly produce or acquire dead cells, cell debris, and excess lipoproteins, which belong to the family of damage-associated molecular patterns (DAMPs), tissues can become spontaneously inflamed through activation of DAMP receptors such as Toll-like receptors (TLRs). 3 Resident macrophages are responsible for clearing DAMPs and preventing unnecessary inflammation. Thus, investigations to delineate the mechanisms by which tissue-resident macrophages differentiate and are maintained are critical for a fundamental understanding of tissue homeostasis. The mechanisms by which tissue-resident macrophages are uniquely differentiated toward specific types have begun to be elucidated. 1,4 However, although it is clear that tissue-resident macrophages are maintained separately from the bone marrow hematopoietic system, how their population is regulated or maintained is still largely undetermined. TAK1 (MAP3K7) is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family, and is activated by a diverse set of inflammatory stimuli including inflammatory cytokines, TNF and IL-1, and TLR ligands. 5 In mouse models, tissue-specific deletion of the Tak1 gene causes cell death resulting in tissue injury in multiple tissues including the epidermis, the intestinal epithelium, and the vascular endothelium. [6][7][8] A profound increase of reactive oxygen species (ROS) is causally associated with Tak1 deficiencyinduced cell death. [9][10][11] The mechanism by which Tak1 deficiency disrupts cellular redox homeostasis is not yet fully understood; however, impairment of several intracellular signaling cascades and transcription factors including but not limited to NF-κB, MAPKs including p38 and JNK, and the antioxidant transcription factor Nrf2 has been implicated in increased ROS. 5,12 Interestingly, recent studies have revealed that not all tissues or cell types are damaged or killed by Tak1 deletion; for example, Tak1-deficient neurons do not exhibit any abnormalities, 13 and some hematopoietic cells seem to be highly resistant to Tak1 deficiency as discussed below. Thus, sensitivities to Tak1 deficiency vary depending on cell type and the cellular context. It still remains to be determined which cell types are sensitive to Tak1 deficiency and the mechanism(s) by which Tak1-deficient cells die. In the hematopoietic system, TAK1 is involved in maintenance of several specific cell types. We previously demonstrated that Tak1 deficiency impairs adult hematopoietic stem cell (HSC) maintenance. 14 Competitive transplantation assays showed that Tak1-deficient bone marrow cells in adult mice are incapable of repopulating any types of hematopoietic cells. 14,15 In contrast, T or B cell-specific deletion of Tak1 skews subset populations of T and B cells but does not cause overt cell death in the in vivo setting. [16][17][18][19] Mice with myeloidspecific deletion of Tak1 were generated and characterized by two groups using the LysM-Cre deleter strain. 20,21 These mice exhibit an increased circulating neutrophil population and develop splenomegaly, and no overt increase in cell death is observed in vivo. 20,21 However, as the LysM-Cre system is not highly effective in several types of myeloid cells including resident macrophages [22][23][24] and its inefficient recombination of floxed genes is known to cause artificial consequences, 25 the role of TAK1 in hematopoietic cells should be further evaluated in other gene deletion systems.
In the current study, we investigated how TAK1 participates in the hematopoietic system by using the Vav-Cre system, which is expressed in all the hematopoietic compartments. 26 Specifically, Vav1 (a GDP/GTP nucleotide-exchange factor for Rho/Rac) is expressed in erythro-myeloid progenitors and fetal HSCs that originate in the yolk sac during early embryogenesis, presumably starting around embryonic day 7. 27 Endothelial cells also originate from the hemogenic endothelial cells. 28,29 However, Vav1 is expressed only in erythro-myeloid progenitors and fetal HSCs but not in endothelial cells, and Vav-Cre is thus suitable for the characterization of target genes in the embryonic hematopoietic system without affecting the endothelium. Furthermore, as erythro-myeloid progenitors give rise to tissueresident macrophages, this system provides the means to characterize target genes in tissue-resident macrophages. 22

Hematopoietic-specific
Tak1 deficiency impairs clearance of dead cells and causes perinatal lethality. TAK1 is required for proper development of the hematopoietic system and maintenance of adult HSC. 14-21 However, our understanding of the roles of TAK1 in various hematopoietic compartments, including those during embryogenesis, remains incomplete. Tie2 (an angiopoietin receptor)-Cre system, can recombine floxed genes during early embryogenesis in hemogenic endothelial cells, early precursors of hematopoietic cells, 30 and is suitable for investigations of the entire hematopoietic system. However, as endothelial cells share the same origin, Tie2-Cre recombines floxed genes in endothelial cells. We previously demonstrated that Tak1 deletion with the Tie2-Cre deleter causes endothelial cell death and early embryonic lethality at embryonic day (E)10.5. 6 We showed that erythrocytes are normally developed but blood vessel regression occurs at E10.5 in these mice, suggesting that endothelial cell autonomous defects but not hematopoietic abnormality cause blood vessel regression. 6 In the current study, we sought to determine the role of TAK1 specifically in the hematopoietic compartment. Vav-Cre was chosen as it is expressed in erythro-myeloid progenitors and fetal HSCs but not in endothelial progenitors. 27,31 We generated Vav-Cre Tak1 flox/flox (Tak1 HKO ) mice and compared them with littermate Cre-negative Tak1 flox/flox and heterozygous Tak1 deletion Vav-Cre Tak1 flox/+ mice. Tak1 HKO mice were found to suffer lethality between E18.5 and postnatal (P) day 1 (Figure 1a). We confirmed that intact TAK1 protein was diminished in all hematopoietic cells including thymocytes at E16.5 ( Figure 1b) and in circulating blood cells at least by E18.5 (Figure 1c). We note here that Tak1-floxed gene expresses a truncated and non-functional form of TAK1 protein (TAK1Δ) when loxP recombination occurs, and TAK1Δ is less stable compare to intact TAK1. 8,17 Heterozygous deletion of Tak1 did not observably reduce the TAK1 protein level, suggesting that a single allele is sufficient for maintenance of the steady-state TAK1 protein level. Consistently, we did not observe any abnormality in Vav-Cre Tak1 flox/+ (Tak1-Het) mice, as we also observed no abnormality in other tissue-specific heterozygous Tak1-deficient mice. [6][7][8]32 Although Tak1 HKO mice die around birth, we did not observe any overt clinical abnormalities except occasional mild cyanosis ( Figure 1d). We performed histological analyses of tissue samples from live Tak1 HKO and their littermate mice at P0. The prominent observable abnormality was found in the thymic medulla, where there was an accumulation of marked dead cell debris (Figure 1e, Supplementary Figures S1 and S2). We also note that the size of lung alveoli in Tak1 HKO animals was reduced compared with that in the littermate controls (Supplementary Figure S3), which may be associated with cyanosis. By contrast, other organs were architecturally indistinguishable from those in control littermates (Supplementary Figure S3). We also performed histopathological evaluation of one E18.5 litter consisting of three Tak1 HKO and three control embryos. At E18.5, no abnormality in the overall morphogenesis of Tak1 HKO embryos was observed, indicating that hematopoietic TAK1 is not required for embryogenesis. No increase in apoptotic or necrotic cells was observed in the liver, intestine, kidney, heart, spleen, lung, brain and spleen. Thus, hematopoietic-specific Tak1 deletion does not impair normal morphogenesis or increase of cell death during embryogenesis, but causes a destruction of thymus and an abnormality in the lung architecture around birth.
Hematopoietic-specific deletion of Tak1 does not impair normal development of lymphocytes and myeloid cells.
To determine the cause of Tak1 HKO thymus abnormality, we first asked if Tak1 deficiency causes any abnormalities in hematopoietic cell compartments within the systemic immune system. We began by analyzing leukocytes in circulating blood and spleen isolated from live P0 Tak1 HKO and littermate control mice ( Figure 2). No difference in the proportions of CD11b + (myeloid) and CD11b − (non-myeloid, including lymphoid) cells was observed between Tak1-deficient mice and controls in the circulating blood or the spleen (Figures 2a,  b, e and f). Among myeloids, CD11b + Ly6C hi (monocytes) and CD11b + Ly6G + (neutrophils) were similarly observed in the spleen and blood of Tak1 HKO and controls mice (Figures 2a and e). Although Tak1 HKO exhibited a trend of reduced myeloid population (Figures 2c and g), neutrophils TAK1 regulates macrophages Y Sakamachi et al were not altered by Tak1 deletion. These results demonstrate that TAK1 is dispensable for normal hematopoietic development of both myeloid and lymphoid cells in the spleen and circulating blood at least around birth in mice with exception of a slight decrease in monocyte population. Hence, the thymic abnormality in Tak1 HKO is not caused by defects in the systemic hematopoiesis.
Hematopoietic-specific deletion of Tak1 diminishes thymic and pulmonary macrophages. We next focused on the thymus. Accumulation of cell debris might be caused by increased thymocyte death during T-cell maturation in the thymus. We thus analyzed T-cell populations in the thymus. If T cells were dying during maturation, the number of CD4 + CD8 + double positive and CD4 + CD8 − and/or CD4 − CD8 + single positive cells should be decreased by Tak1 deficiency. Unexpectedly, we found increased CD4 + CD8 + double positive cells in Tak1 HKO thymus, whereas the CD4 − CD8 − double negative population was decreased (Figures 3a  and b). The numbers of mature CD4 + CD8 − and CD4 − CD8 + single positive cells were comparable between control and Tak1-deficient thymus (Figures 3a and b). Thus, Tak1 deficiency does not impair T-cell development and maturation. The CD4 − CD8 − double negative populations contain both naive T cells and other cell types in the thymus including thymic macrophages. Thymic macrophages have an indispensable role in clearance of dead thymocytes during T-cell development. 33 Thus, Tak1 deficiency might impair thymic macrophages, which could cause accumulation of cell debris in the thymus. To test this possibility, we analyzed F4/80 + macrophages in the Tak1 HKO and control thymus. The number of thymic macrophages was markedly reduced by Tak1 deficiency (Figures 3c-e), indicating that TAK1 is required for establishment and/or maintenance of thymic macrophages. This suggested the possibility that TAK1 also contributes to integrity of other tissue-resident macrophages. We analyzed F4/80 + macrophages in the lung and spleen of P0 Tak1 HKO and control mice. The number of macrophages was also markedly reduced in the Tak1 HKO lung and spleen compared with the controls (Figures 3f, g and Supplementary  Figure S4). These results demonstrate that TAK1 is required for establishment and/or maintenance of resident macrophages in the thymus, lung and spleen.
Tak1 deficiency impairs lysosomes and kills bone marrow-derived macrophages without exogenous stressors. We next attempted to determine the mechanism by which Tak1 deficiency reduces resident macrophages. It has previously been reported that Tak1-deficient bone marrowderived macrophages (BMDM) spontaneously undergo cell death. 20,34,35 Thus, loss of viability by Tak1 deficiency is likely to be the cause of reduced resident macrophages. However, the mechanism by which Tak1 deficiency spontaneously kills macrophages is still elusive. To gain mechanistic insights, we took advantage of the inducible gene deletion system. We prepared BMDM from mice having the ubiquitous inducible Cre deleter (Rosa26-CreERT) 36 and Tak1-floxed genes (Tak1 iKO ). TAK1 was diminished in Tak1 iKO BMDMs after 3 days treatment of 4-hydroxytamoxifen (4-OHT) (Figure 4a). Tak1-deficient cells including fibroblasts and keratinocytes die with apoptosis, and RIPK3-dependent necroptosis has also been implicated in cell death by Tak1 deficiency when Tak1deficient cells are treated with a pan-caspase inhibitor, Z-VAD-FMK (Z-VAD). 5 However, we unexpectedly found that Tak1-  (Figure 4b). Thus, Tak1-deficient macrophages die primarily not through either apoptosis or necroptosis.
We explored other modes of cell death, including pyroptosis and lysosome malfunction-induced cell death. The pancaspase inhibitor Z-VAD-FMK, which inhibits the pyroptosis mediator caspase 1 and caspase 11, 37 did not block Tak1deficient BMDM death (Figure 4b), suggesting that pyroptosis is not the major cause of cell death. Notably, however, Tak1 iKO BMDMs exhibited abnormal lysosomal architecture (Figure 4c), and co-localization of the lysosomal protease, cathepsin B and a lysosome marker, lamp1, was disrupted (Figures 4c and d). Furthermore, acridine orange staining revealed that Tak1 deficiency increased dysfunctional lysosomes with an elevated pH (yellow and green) (Figures 4e and f). Such abnormalities in lysosomes were not observed in 4-OHT treated BMDMs having a different floxed gene together with Rosa26-CreERT (Supplementary Figure S5), indicating that neither 4-OHT nor Cre are the cause of the lysosome abnormalities. Thus, Tak1 deficiency is the cause of lysosomal abnormality. We asked  (Figure 4g, left graph), suggesting that inhibition of cathepsins generally improves BMDM survival and/or proliferation. Importantly, inhibition of cathepsin B increased the number of Tak1 iKO BMDMs with higher efficiency (3-fold) than wild type BMDMs, suggesting that cathepsin activity is responsible, at least in part, for Tak1 iKO BMDM death. The mouse macrophage cell line, RAW264.7, was also killed by a selective inhibitor of TAK1, 5z-7oxozeanol 38 (Figure 4g, right graph). In RAW264.7 cells, inhibition of cathepsin B alone did not alter cell viability but partially rescued TAK1 inhibitor-induced cell death (Figure 4g). Taken together, these results demonstrate that Tak1 deficiency impairs lysosomes, and that lysosomal dysfunction contributes to macrophage death. TNF is the trigger of cell death. Tak1-deficient BMDMs die without any exogenous stimuli in standard culture conditions. TAK1 is activated by a number of stressors including inflammatory cytokines and TLR ligands including dead cell-derived molecules (i.e., DAMPs). Macrophages produce inflammatory cytokines, TNF and IL-1, and dead cells are unavoidably present in the medium during isolation and culture of BMDMs. We asked whether TNF, IL-1 or TLR signaling is involved in Tak1-deficient BMDM death. To examine the TNF pathway, we utilized TNF receptor 1 (TNFR1)-deficient (Tnfr1 −/− ) mice. 39 IL-1 and TLR pathways share the same adaptor MyD88, and some TLRs additionally utilize another adaptor, TRIF. 40 To examine these pathways, we generated Rosa26-CreERT Tak1 flox/flox Myd88 flox/flox and Rosa26-CreERT Tak1 flox/flox Trif −/− mice. An earlier study using the LysM-Cre system reported that TNF receptor deficiency rescues macrophage differentiation in myeloidspecific Tak1 deletion bone marrow cells. 35 However, involvement of TNF in macrophage viability after the completion of differentiation was not clear. We found that Tnfr1 deficiency effectively albeit incompletely rescued Tak1 iKO BMDM death (Figure 5a), whereas deletion of either Myd88 or Trif did not restore cell viability (Figure 5a). This suggests that TNF is, at least in part, the cause of Tak1 iKO BMDM death. Furthermore, we found that only a small proportion of cells exhibited abnormal localization of cathepsin B in the Tnfr1-deficient background (Figure 5b). Nonfunctional lysosomes with elevated pH were not increased by Tak1 deletion on the Tnfr1 −/− background (Figures 5c and d).
Collectively, these results suggest that TNF is the major cause of lysosomal dysfuntion, and associated cell death, in Tak1-deficient BMDMs.
Tnfr1 deletion partially rescues developmental abnormalities and loss of resident macrophages. We finally examined whether TNF-induced cell death is the cause of diminished resident macrophages in Tak1 HKO mice. We generated and analyzed Tak1 HKO Tnfr1 −/− mice. Tnfr1 −/− mice are normal under standard housing conditions, and there is no overt abnormality in morphogenesis. 41 TAK1 was effectively depleted in the Tak1 HKO Tnfr1 −/− thymus at P0 (Figure 6a), similar to Tak1 HKO shown in Figure 1b. However, the cell debris, which was profoundly observed in the Tak1 HKO thymus (Figure 1e), was not seen in the Tak1 HKO Tnfr1 −/− thymus (Figure 6b). The number of thymic macrophages was still reduced in the Tak1 HKO Tnfr1 −/− thymus (Figure 6c), but it was improved compared with those in Tak1 HKO thymus (Figures 3d and f). Furthermore, the number of pulmonary macrophages was restored by Tnfr1 deficiency (Figure 6d). These results demonstrate that, although other unidentified causes contribute to reduction of Tak1-deficient macrophages, TNF is one of the major triggers of diminished resident macrophages in Tak1 HKO mice. However, we note that animal mortality was not rescued by Tnfr1 deletion (Figure 6e), suggesting that reduction of macrophages is not the prominent cause of animal mortality, and also that the Collectively, although we could not identify the cause of animal mortality, our results reveal that TAK1 is a critical regulator of macrophage maintenance in the perinatal hematopoietic system by preventing TNF-induced lysosomal damage.

Discussion
TAK1 is a signaling molecule that both promotes inflammatory responses and guards against cell death during inflammation in several cell types. We previously reported that Tak1deficient epithelial and endothelial cells die, causing severe tissue damage. [6][7][8]10,11 In the current study, we show that Tak1 deficiency uniquely causes cell death in macrophages among hematopoietic cells during embryogenesis. Although Tak1deficient thymocytes, splenocytes and circulating myeloid cells develop normally, Tak1 deficiency diminishes thymic and lung-resident macrophages. This raises a question as to why certain cell types are selectively sensitive to Tak1 deficiency. One possibility is that proliferative cells may be sensitive to Tak1 deficiency. This idea is consistent with the fact that constantly renewing tissues such as the epidermis and the intestinal epithelium are highly sensitive to Tak1 deficiency, 7,8 whereas neurons, which are mostly post-mitotic, are resistant to Tak1 deficiency. 13 However, it is inconsistent with the fact that embryonic hematopoietic progenitors are highly proliferative but the hematopoietic system developed normally in Tak1 HKO mice. Furthermore, cultured fibroblasts are also highly proliferative under standard culture conditions; however, Tak1 deletion does not impair cell viability. 5 In contrast, adult HSCs are known to be slowly self-renewing cells, but they are effectively depleted by Tak1 gene deletion. 14 Similarly, cultured macrophages (BMDMs) are mostly postmitotic or very slow growing after completion of the differentiation processes, but they die upon Tak1 gene deletion. Thus, cell proliferation is unlikely to be the determinant of the sensitivity to Tak1 deficiency. Tak1-deficient fibroblasts and keratinocytes undergo cell death when they are treated with TNF. 8,42 TNF treatment induces accumulation of ROS in Tak1-deficient cells. Similarly, ablation of Tak1 activity in BMDMs highly upregulates ROS. 43 TNF triggers cellular ROS production and phagocytic macrophages produce ROS. 44,45 Furthermore, ROS participate in adult HSC renewal and differentiation, 46,47 which may be further upregulated by Tak1 deficiency. These issues raise the possibility that ROS may be the determinant of Tak1 sensitivity. Although all cells constantly produce ROS as byproducts of respiration, cells producing ROS beyond a certain level such as macrophages and TNF-treated fibroblasts may perhaps be killed if functional TAK1 is absent. We have previously reported that several cellular antioxidant enzymes such as glutamate-cysteine ligase catalytic subunit and NAD (P)H quinone dehydrogenase 1 and an antioxidant transcription factor Nrf2 are downregulated by Tak1 deficiency. [10][11][12] Although the molecular mechanism by which TAK1 regulates ROS is not fully understood, ablation of TAK1 seems to reduce the capacity of the cellular antioxidant system, resulting in accumulation of a cell-killing level of ROS if cells actively produce ROS.
Increased ROS are commonly and causally associated with cell death in Tak1-deficient keratinocytes, intestinal epithelial cells, and macrophages; however, the pathways of cell death vary depending on the cellular context. Caspase activity is profoundly upregulated in Tak1-deficient epidermis and intestinal epithelium as well as TNF-treated Tak1-deficient keratinocytes and fibroblasts, 10,11,42 indicating that they die predominantly through apoptosis. Earlier studies also implicate TAK1 in programmed necrosis, so-called necroptosis, in the intestinal epithelium and macrophages. 32,35 Here, we show that Tak1 deficiency causes lysosomal abnormality in macrophages. ROS are implicated in many types of cells death including apoptosis, necroptosis, and lysosomal rupture. [48][49][50] ROS could trigger apoptosis through activation of mitochondrial membrane permeabilization and ROSinduced necroptotic protein assembly in macrophages. Our results demonstrate that apoptosis and necroptosis are not major forms of Tak1-deficient macrophage death. Phagocytic macrophages possess highly active phagosome-lysosomes, and lysosomal enzymes are highly expressed in macrophages. It is therefore possible that, due to such high lysosomal activity, Tak1 deficiency may predominantly cause lysosomal damage through accumulation of ROS in macrophages but not other cell types.
Tissue-resident macrophages support tumor growth by clearing undesired molecules, supplying growth factors, and inducing angiogenesis. 51 Thus, the cell type specific sensitivity to Tak1 deficiency may be useful for developing approaches to manipulate macrophages in tumors. However, inhibition of TAK1 activity such as application of pharmacological inhibitors of TAK1 is anticipated to cause a number of undesired consequences in normal tissues based on the mouse studies using tissue-specific deletion of Tak1. The potential deleterious conditions include epithelial tissue damage 7,8 and skewing of adaptive immune cell populations, [16][17][18][19] which could lead to chronic inflammatory diseases if inhibition of TAK1 is prolonged. In contrast to prolonged inhibition of TAK1, temporary inhibition or inducible deletion of Tak1 gene has thus far exhibited promising outcomes. Epidermal-specific inducible Tak1 deletion can induce tumor regression in skin papilloma but does not cause observable injury in the normal skin. 9 Treatment with a selective TAK1 inhibitor 5z-7oxozeanol 38 effectively blocks tumor growth without overt toxicity. 52 Thus, although inhibition of TAK1 must be entertained with considerable caution, regulated inhibition of TAK1 may be potentially useful to selectively kill macrophages without affecting adaptive immunity in certain contexts.

Materials and Methods
Mice. Tak1-floxed (Tak1 flox/flox ) mice have been described previously, 17 and were backcrossed at least seven times to C57BL/6. Vav-Cre (Jax mice, B6.Cg-Tg(Vav1icre)A2Kio/J), 26,53 Rosa26-CreERT (Jax mice, B6;129-Gt(ROSA)26Sortm1 (cre/ERT)Nat/J), 36 Tnfr1 −/− (B6.129-Tnfrsf1atm1Mak/J) 39 and Ripk3 −/−54 mice were bred in our facility to produce the genotypes used. For characterization of Tak1 HKO (vav-Cre Tak1 flox/flox ), littermate and wild type (no-Cre Tak1 flox/flox ) and heterozygous Tak1 deletion (vav-Cre Tak1 flox/+ ) mice, which were phenotypically indistinguishable, were used as controls. Rosa26-CreERT Tab2 flox/flox BMDMs were also used as controls, which were described previously. 34,55 Animal viability was calculated based on the assumption that Tak1 HKO mice were born at a Mendelian ratio. All animal experiments were conducted with the approval of the North Carolina State University Institutional Animal Care and Use Committee. and acridine orange (ThermoFisher Scientific, Waltham, MA, USA) were used. The TAK1 kinase inhibitor, 5Z-7-oxozeaenol (5Z) was described previously. 38 Histology and immunofluorescence staining of embryos. Embryo and neonate fixation and staining were performed using the method described previously. 57 Briefly, E18.5 and P0 mice were killed, blanched in boiling water for 20 s, and immersed in an ice water bath to permit the removal of the epidermis. An incision was also made from the right clavicle to the pubic bone, opening the thoracic and abdominal cavities for improved penetration of fixative and processing reagents. Animals were fixed in Bouin's solution for 48 h at room temperature on a shaker followed by multiple 70% ethanol washes for 30 min each to remove excess fixative from the tissue before histiologic processing. E18.5 embryos from one litter consisting of three Tak1 HKO and three controls (no-Cre and heterozygous deletion) were step-sectioned dorsal to ventral at 300 μms, six serial sections (6 μms) were collected were stained with haemotoxylin and eosin, and the sections were pathologically evaluated by two board certified pathologists. For immunofluorescence staining of F4/80, 4% paraformaldehyde fixed embryo tissues were embedded in optimum cutting temperature (OCT) media, and 4-6 μm cryosections were stained using anti-F4/80 (1 : 300) overnight at 4°C. Bound antibodies were visualized by the Alexa Fluor 594 and 488 fluorescence dye-conjugated secondary antibodies (ThermoFisher Scientific). For quantification, more than 10 randomly digital images from each sample with the same exposure time were used.
Flow cytometric analysis. Whole blood, thymus and spleen cells were isolated with Hank's balanced salt solution without magnesium and calcium (HBSS ( − )). The cells were then suspended in 0.83% ammonium chloride for 5 min at room temperature to lyse red blood cells and washed with HBSS( − ). Cells were resuspended in HBSS( − ) and filtered to obtain single cell suspension, which was confirmed by a pulse geometry gate, FSC-A versus FSC-H. The cells were incubated for 20 min on ice with anti-CD16/32 antibody to block FcγRII/III, followed by incubation with fluorochrome-conjugated antibodies against cell surface antigens as described above. After labeling, cells were washed once with HBSS( − ), resuspended in HBSS( − ) and analyzed on FACS LSRII (BD Biosciences, Franklin Lakes, NJ, USA) and FlowJo (Version 10.1, FlowJo.LLC, Ashland, OR, USA). Debris was removed using a standard FSC versus SSC gating, and the subsequent gating strategy was shown in Figure 2.