Figure 3

From: The novel long intergenic noncoding RNA UCC promotes colorectal cancer progression by sponging miR-143

Figure 3

Downregulating UCC induces CRC cell apoptosis and G1/S arrest and inhibits invasion. (a) The effect of UCC knockdown on apoptosis of SW620 and SW480 cells was determined by measuring the percentage of Annexin V-stained cells using fluorescence correlation microscopy (FCM). Left: 72 h after treatment with negative control and UCC siRNA, Right: ratio of early apoptotic cells was collected and presented in the column chart. (b) The cell cycle distribution after knockdown of UCC was determined by PI staining and FCM in SW620 and SW480 cells. (c) TUNEL assays showed that UCC knockdown induced apoptosis of xenograft tumor cells. (d) Silencing UCC decreased SW620 and SW480 cell mobility. Left: the width of the scratch-wounded cell monolayer was recorded at 0 and 48 h after wounding via photography. Right: the relative migration distance presented in the column chart. (e) Downregulation of UCC inhibited CRC cell invasion based on Transwell assays. The bars indicate mean±S.D. *P<0.05. All the experiments were repeated three times