Figure 2

From: The novel long intergenic noncoding RNA UCC promotes colorectal cancer progression by sponging miR-143

Figure 2

UCC knockdown inhibits CRC cell growth in vitro and in vivo. (a) UCC expression levels were suppressed by specific siRNAs in CRC cells. (b) Growth curves of SW620 and SW480 cells after transfection with si-UCC or si-NC were determined via MTS assays. (c) The anchorage-independent growth of SW620 and SW480 cells was assessed via colony formation assays. (d) Cell proliferation was evaluated using EdU incorporation assays. Proliferating cells were labeled with EdU. Scale bar: 200 μm. (e) Effects of UCC knockdown on tumor growth after 4 weeks in vivo (n=5 per group). Upper: negative control cells. Lower: representative images of tumors formed in nude mice subcutaneously injected with UCC-silenced SW620 cells. (f) Growth curves of xenograft tumors after subcutaneous injection of mice with UCC-silenced SW620 or negative control cells. The tumor volumes were measured every 5 days after inoculation. (n=5). (g) Immunohistochemical staining showed that UCC knockdown decreased the Ki-67 proliferation index. The data were represented as the mean±S.D. of three independent experiments in vitro or five independent experiments in vivo. *P<0.05, **P<0.01 by Student’s t-test