Figure 5 | Cell Death & Disease

Figure 5

From: miRNA-558 promotes gastric cancer progression through attenuating Smad4-mediated repression of heparanase expression

Figure 5

miR-558 facilitates the tumorigenesis and aggressiveness of gastric cancer cells in vivo. (a) The in vivo growth curve of SGC-7901 cells (1 × 106) stably transfected with empty vector (mock) or miR-558 precursor, and those co-transfected with Smad4 in athymic nude mice (n=5 for each group), and xenograft tumours after hypodermic injection for 4 weeks. (b) Representation (left) and quantification (right) of xenograft tumours formed by hypodermic injection of SGC-7901 cells stably transfected with mock or miR-558 precursor, and those co-transfected with Smad4. (c) Immunohistochemical staining (left) and quantification (right) of CD31 expression within tumours formed by hypodermic injection of SGC-7901 cells stably transfected with mock or miR-558 precursor, and those co-transfected with Smad4. Scale bars: 100 μm. (d) Representation (left, arrowhead) and quantification (right) of lung metastasis of nude mice with injection of SGC-7901 cells (0.4 × 106) stably transfected with mock or miR-558 precursor, and those co-transfected with Smad4 via the tail vein (n=5 for each group). Scale bars: 100 μm. (e) Kaplan–Meier survival plots of nude mice with injection of SGC-7901 cells (0.4 × 106) stably transfected with mock or miR-558 precursor, and those co-transfected with Smad4 into the tail vein of athymic nude mice (n=5 for each group). *P<0.01 versus mock

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