HSV-1-induced activation of NF-κB protects U937 monocytic cells against both virus replication and apoptosis

The transcription factor nuclear factor-kappa B (NF-κB) is a crucial player of the antiviral innate response. Intriguingly, however, NF-κB activation is assumed to favour herpes simplex virus (HSV) infection rather than restrict it. Apoptosis, a form of innate response to viruses, is completely inhibited by HSV in fully permissive cells, but not in cells incapable to fully sustain HSV replication, such as immunocompetent cells. To resolve the intricate interplay among NF-κB signalling, apoptosis and permissiveness to HSV-1 in monocytic cells, we utilized U937 monocytic cells in which NF-κB activation was inhibited by expressing a dominant-negative IκBα. Surprisingly, viral production was increased in monocytic cells in which NF-κB was inhibited. Moreover, inhibition of NF-κB led to increased apoptosis following HSV-1 infection, associated with lysosomal membrane permeabilization. High expression of late viral proteins and induction of apoptosis occurred in distinct cells. Transcriptional analysis of known innate response genes by real-time quantitative reverse transcription-PCR excluded a contribution of the assayed genes to the observed phenomena. Thus, in monocytic cells NF-κB activation simultaneously serves as an innate process to restrict viral replication as well as a mechanism to limit the damage of an excessive apoptotic response to HSV-1 infection. This finding may clarify mechanisms controlling HSV-1 infection in monocytic cells.

The transcription factor nuclear factor-kappa B (NF-κB) plays a fundamental role in the innate antiviral response, acting as a hub network in signal transduction that connects cellular sensors of viral invasion with effector mechanisms of antiviral defence. 1,2 Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2) are two closely related large DNA viruses that can infect a variety of cell types, using insidious strategies to modify the molecular organization of the host cell to their own advantage. 3 HSV-1 can activate NF-κB. 4 Right after infection, NF-κB activation is independent on virus entry or viral protein expression, as we first demonstrated, 5 and is triggered by viral structural proteins, such as gD and gH/gL, or the UL37 tegument protein and their interactions with specific receptors. [5][6][7][8][9][10][11] Later, beginning at 3-6 h post infection (p.i.), NF-κB activation requires the de-novo expression of viral proteins. [12][13][14] Noticeably, NF-κB activating components in HSV-1 have not been related with an antiviral role of NF-κB signalling, but rather with a subtle strategy of virus invasiveness. 15,16 Various studies indeed suggested that inhibition of NF-κB activation decreased the efficiency of HSV-1 replication in permissive cells. 15,[17][18][19] However, all data showing a pro-virus connotation for NF-κB activation during HSV-1 infection were obtained in fully permissive cell lines. No data are actually available for functions exerted by NF-κB with regard to HSV-1 infection of monocytic cells.
Apoptosis is another evolutionary conserved form of innate response to virus infection and viruses belonging to different families evolved efficient strategies to counteract it. [20][21][22] Actually, numerous proteins of HSVs capable of preventing apoptosis during infection have been identified. 23 Nevertheless, although inhibition of apoptosis is the prevalent outcome of HSV infection in permissive cells, monocytic and dendritic cells as well as lymphocytes have been shown to undergo apoptosis after infection by HSV. [24][25][26][27][28][29][30] All these cell types share the common characteristic of undergoing lowproductive or abortive infections following exposure to HSV, for reasons that have not been completely elucidated. Interestingly, we have recently demonstrated that apoptosis by HSV-1 in monocytic cells depends on Bax/Bak and the BH3-only Puma protein. 31 In addition, Bcl-2 overexpression in U937 monocytic cells infected with HSV-1 31 or HSV-2 32 leads to an increase of virus yield, further supporting the notion that virus replication in this cell type can be strongly affected by modulating the apoptotic pathway.
Interestingly, a complex interplay exists between NF-κB signalling and apoptosis. In fact, NF-κB was shown to promote both inhibition and induction of apoptotic cell death depending on the context. 33 During HSV infection, NF-κB translocation to the nucleus has been associated with apoptosis prevention in permissive cells. 34,35 Contrasting results, however, were obtained by another group. 36 With regard to monocytic cells, we demonstrated that activation of NF-κB following infection with HSV-1 as well as upon exposure to HSV-1 glycoprotein D prevented Fas-or staurosporine-induced apoptosis, and this effect was associated with an increased expression of NF-κBdependent anti-apoptotic genes such as c-IAP2, FLIP and survivin. 5,37 Furthermore, we recently reported that cells that expressed a dominant negative (DN) form of IκBα displayed high levels of apoptosis when infected with wild type HSV-1. 31 These results support a role for NF-κB activation in preventing apoptosis during HSV infection that can vary with respect to the context and the ability of the cells to sustain virus replication. Thus, resolving the intricate interplay between NF-κB signalling, apoptosis and HSV-1 replication remains an intriguing issue to better understand the complex processes controlling HSV infectious cycle and the restriction of infection in some cell types.
The primary objective of this study was to investigate the relationships among NF-κB activation, viral replication and apoptosis in monocytic cells infected by HSV-1. For this purpose, we used U937 monocytic cells, known to sustain a restricted virus replication cycle and to be susceptible to apoptosis following HSV-1 infection.

Results
HSV-1 infection triggers NF-κB activation in control U937 transfectants but not in U937 cells stably transfected with a DN murine IκB. To investigate the effects of NF-κB activation on HSV-1 replication and apoptosis in monocytic cells, we utilized U937 cells in which NF-κB was inhibited by stably expressing a DN form of murine IκBα (U937-DN-IκB) and vector control U937 cells (U937-pcDNA). 5 We first determined the kinetics of NF-κB using electrophoretic mobility shift assays (EMSA). Note that for these experiments the indicated times refer to the first exposure to the virus and not to the end of the adsorption time, as for all other experiments. Figure 1a shows that NF-κB binding activity was increased as early as 30 min after first exposure to HSV-1 at a multiplicity of infection (MOI) of 50 plaque forming units (PFU)/cell in U937-pcDNA as compared to mock-infected cells. Thereafter the binding activity diminished (3 h) but reappeared during a later phase of HSV-1 infection (6-18 h) (Figure 1b). Mock-infected U937-pcDNA showed very low levels of basal ΝF-κB activation. Complete absence of NF-κB specific bands and accumulation of the free DNA probe at the bottom of the gels in either HSV-1-infected or mock-infected U937-DN-IκB cells indicate that DN-IκB stable transfection rendered U937-DN-IκB cells homogenously refractory to NF-κB activation (Figures 1a and b). Figure 1c shows a comprehensive time course of NF-κB binding activity in U937-pcDNA, based on densitometric ImageJ evaluation of EMSA gels. These data indicate the expected biphasic behaviour of HSV-1-induced NF-κB activation in U937 monocytic cells, as previously reported in different experimental cell systems. 38 No appreciable optical density was detected in EMSA gels from U937-DN-IκB cells, confirming the reliability of our experimental system (data not shown). Such an optimal inhibition of NF-κB binding activity was not achieved with the IkBα phosphorylation inhibitor Bay 11-7085 at concentrations that did not affect the viability in U937 cells (data not shown). We therefore preferred to inhibit NF-κB by DN IκB expression for most of experiments.
HSV-1 replicates more efficiently in U937 cells with disrupted NF-κB activation. Having ascertained that HSV-1 infection triggers NF-κB activation in U937-pcDNA but not in U937-DN-IκB cells viral gene expression and replication were compared between the two cellular systems by various techniques. First, cells were infected at different MOIs and viral gene expression was assessed at 24 h p.i. by immunofluorescence analysis using a HSV-1 gD-specific antibody. U937-pcDNA cells, even at higher MOIs , were fairly refractory to HSV-1 replication, with only about 10% gD positive cells at 24 h p.i. (Figure 2a In addition, the dramatic difference in gD positivity between U937-pcDNA and U937-DN-IκB cells was documented by flow cytometry experiments at 24 h p.i. with HSV-1 ( Figure 2b). When we extended the HSV-1 infection time to 48 h, a similar trend was observed. Both the gD positivity and the viral titres were remarkably higher in the U937 cells refractory to NF-κB activation as compared with those in which NF-κB activation was normal ( Figure 2c). To get further information on viral replication rate of HSV-1 in monocytic cells with suppressed NF-κB activation, the transcriptional induction of glycoprotein I (gI) of HSV-1 was assessed by real-time RT-PCR analysis during the early phase of infection. As shown in Figure 2d, whereas gI mRNA levels were almost undetectable at 3 h p.i. in U937-pcDNA cells, U937-DN-IκB cells already exhibited a transcriptional induction of gI at the same time that further increased until 6 h p.i. Finally, we monitored changes in the protein levels of the immediateearly protein (ICP0) and of a late protein (gD) by western blot analysis following HSV-1 infection with 50 MOIs in U937-pcDNA and U937-DN-IκB cells as well as in U937-pcDNA cells treated with the NF-κB inhibitor BAY-117085. Again, both the ICP0 and gD protein levels were substantially higher in the cells where NF-κB activation was inhibited after 24 and 48 h p.i. (Figure 2e). This difference was confirmed by densitometric analysis of the bands from three experiments after normalization with beta tubulin (Supplementary Figure 1). No gD positivity was detected in U937-pcDNA and U937-DN-IκB cells infected with UV-irradiated virus at any time assayed (Figures 3b and d), while in cells infected with non-irradiated HSV-1 gD positivity was detected as early as at 6 h after infection in the case of U937-DN-IκB cells and at a lesser extent at 12 h after infection in U937-pcDNA cells (Figures 3a and c), confirming that increased gD positivity was due to de-novo transcription and not due to residual gD from virus inoculum. Considering that HSV-1 utilizes the herpesvirus entry mediator (HVEM) receptor to infect monocytic cells, the surface expression of this receptor in U937-DN-IκB and in U937-pcDNA was assessed by flow cytometry analysis, but only a slight difference, too small to explain the remarkable difference in viral replication, was detected between the two cell lines (Supplementary Figure 2). However, to be sure that protection against HSV-1 infection exerted by NF-κB activation did not occur at virus entry level, we titrated the unabsorbed virus in the supernatants collected from cultures of both cell lines infected with HSV-1 at an MOI of 50 PFU/cell at the end of the adsorption period. No difference in residual virus was seen from the supernatants of U937-DN-IκB as compared to U937-pcDNA transfectants (Supplementary Figure 3).
HSV-1 induces higher levels of apoptosis in U937 cells when NF-κB activation is prevented. The paradigm in virus infection is that levels of virus replication should be inversely correlated with levels of apoptosis, as we previously observed in Bcl-2 overexpressing U937 cells infected with HSV-2 32 and in a less pronounced manner with HSV-1. 31 Given that disruption of NF-κB activation enhanced HSV-1 replication in monocytic cells we could expect that levels of apoptosis should be concomitantly diminished. However, NF-κB is considered a survival factor and prevention of its activation should enhance apoptosis. 5 We, then, determined the extent of apoptosis in U937-pcDNA cells and U937-DN-IκB cells infected with HSV-1 at different MOIs (0-50) at 24 or 48 h p.i. by either counting apoptotic versus intact nuclei under the microscope (Figure 4a) or performing flow cytometry analysis using propidium iodide staining of isolated nuclei ( Figure 4b). As shown in Figure 4a, apoptosis was remarkably higher in HSV-1-infected U937-DN-IκB, either at 24 h p.i. or at 48 h p.i., as compared to control U937-pcDNA cells, with a peak of about 50% apoptosis in U937-DN-IκB cells at 48 h p.i with an MOI of 50 PFU/cell. Flow cytometry analysis confirmed that U937 cells with impaired NF-κB activation displayed more apoptotic, sub G1 nuclei following HSV-1 infection at 24 h p.i., Moreover, while caspase-3 was readily cleaved into its active p17 form in HSV-1-infected U937-DN-IκB cells, such a cleavage was hardly seen in the control U937-pcDNA cells (Figure 4c). In addition, exposure to replication-incompetent UV/HSV-1, differently from intact virus, did not give rise to detectable levels of apoptosis, as revealed by Hoechst staining (Figure 3). Overall, these results demonstrate that NF-κB activation restrains the apoptotic response to HSV-1 infection in monocytic cells.
Higher levels of apoptosis were associated with lysosomal membrane permeabilization (LMP) in U937 cells with impaired NF-κB activation. Lysosomal membrane  39 To better characterize enhanced levels of cell death induced by wild type HSV-1 in monocytic cells in which NF-κB activation was impaired, we therefore determined the extent of LMP by staining our mock-or HSV-1-infected cells with the lysosomotropic probe acridine orange. At 24 and 48 h p.i. the HSV-1-infected U937-pcDNA cells, which were quite resistant to apoptosis, did not show any reduction of red fluorescence, indicating that LMP did not occur (Figure 5a, left panel). By contrast U937-DN-IκB cells significantly lost their red acridine orange staining with a concomitant increase in green fluorescence at 48 h p.i. (Figure 5a, right panel). Thus, in these cells, apoptosis sensitivity towards HSV-1 was associated with LMP and a possible alteration of lysosomal function. To confirm this finding in U937-DN-IκB cells, the experiment was repeated with the lysosomotropic probe Lyso TNFα and IFNα do not play a major role in NF-κBdependant protection against both virus replication and apoptosis in HSV-1-infected monocytic cells. In the next step we focused on the cellular events involved on protection exerted by NF-κB activation against virus replication and apoptosis in monocytic cells infected by HSV-1. First, the transcriptional profile of genes known to play a role in the antiviral innate response was compared between U937-pcDNA and U937-DN-IκB cells infected with HSV-1 at 3, 6 and 24 h p.i. in parallel with mock-infected time-matched control cells. The genes were grouped by functional activity and the results were expressed as fold change of mRNA expression calculated by dividing the value obtained from HSV-1-infected cells by that obtained from corresponding mock-infected cells (Figure 7). The selection of possibly implicated genes was based on the following arbitrary criteria: (i) genes showing at least a two-fold change in mRNA expression as compared to mock-infected cells, (ii) genes showing a relative mRNA expression that was significantly higher in U937-pcDNA as compared to U937-DN-IκB cells, at least for two out of the three times assayed. As depicted in Figure 7, none of the genes belonging to the chemokine or Toll-like-receptors (TLR) functional groups satisfied both criteria. Particularly, TLR9 expression was even higher in U937-DN-IκB cells as compared to control cells, suggesting the exclusion of this receptor in NF-κB-dependent restriction of HSV-1 infection in U937 cells. Among the interferon (IFN) and the pro-inflammatory cytokine genes, only IFNα and tumor necrosis factor (TNF)-α were taken into consideration based on the adopted criteria. In fact, both IFNα and TNFα were remarkably upregulated in HSV-1 infected U937-pcDNA versus U937-DN-IκB cells at 3 and 24 h p.i.
Since both IFNα and TNFα are well-known NF-κB target genes playing pivotal roles in antiviral innate immunity, in successive experiments neutralizing antibodies directed towards IFNα and TNFα were applied to HSV-1 infected U937-pcDNA and U937-DN-IκB cell cultures before viral replication and apoptosis were assayed at 24 h p.i. As shown in Figure 8a, the differences between vehicle and anti-IFNαtreated U937-pcDNA cell cultures were not statistically significant and no changes were observed in U937-DN-IκB cells (Figure 8a). Thus, as previously seen, gD positivity was largely increased in HSV-1-infected U937-DN-IκB as compared to U937-pcDNA cells but neutralizing antibodies against IFNα or TNFα had no inhibitory effect on this process. Similar results were obtained when virus titration was utilized for evaluating virus replication (data not shown). Also the extent of apoptosis, which again was higher in the U937-DN-IκB as compared to U937-pcDNA cells, was not affected by neutralizing IFNα or TNFα during HSV-1 infection (Figure 8b, left graphs).

Discussion
Previous results indicated that NF-κB activation, rather than playing a role in the host response to virus, increased HSV-1 replication in fully permissive cells. [15][16][17][18][19] Recent studies, however, indicate the existence of at least five HSV-1 proteins, including the virion-host shutoff protein, the UL42 DNA polymerase processivity factor, ICP27, ICP0 and the US3 protein kinase, which are capable of antagonizing NF-κB signalling. [40][41][42][43][44] This antagonizing function does not fit with an unequivocal pro-virus purpose of NF-κB activation during HSV infection. In fact, from an evolutionary point of view, the presence of multiple anti-NF-κB genes in HSV-1 genome suggests that, at least under particular conditions, NF-κB activation could be detrimental for HSV-1 similar to what occurs in most viral infections. Actually, a study reported an increased HSV-1 replication in permissive cells under conditions in which NF-κB activation was presumably inhibited. 45 However, this was not found with the wild type virus, but only for the ΔICP0 mutant virus that cannot counteract NF-κB. Nevertheless, this finding suggests the possibility of an antiviral role for NF-κB in HSV infection, at least in particular conditions. Data reported here definitely demonstrate that the facilitating role of NF-κB activation on wt-HSV-1 replication is not an absolute dogma, but depends on the type of the infected host cells. In fact, our results clearly show that in human U937 monocytic cells in which the NF-κB pathway was functionally ablated specifically by stable transfection of a DN iκBα, replication of wild type HSV-1 occurred much more efficiently than in NF-κB competent control cells. It is known that not all cell types can fully sustain HSV-1 replication following exposure to the virus. Thus, while cell types fully permissive to HSV-1, such as human epithelial cells, produce a high viral yield, those not completely permissive to HSV-1, including monocytic cells, typically generate only low titres of HSV-1. 46 Regarding the NF-κBdependent, down-stream molecular mechanism that restrain virus replication in HSV-1 infected monocytic cells, unfortunately none of the selected, NF-κB-dependent, innateresponse-related genes seemed to play a major role. For IFNα and TNFα we further excluded their major implication. Thus, additional studies are necessary to identify the NF-κBdependent factor/s responsible for HSV-restriction in the majority of monocytic cells.
Regarding the role of NF-κB signalling for the survival/ apoptosis of monocytic cells after HSV-1 infection, one consequence of our results is that restriction of HSV-1 replication in NF-κB-competent monocytic cells is only partially due to levels of apoptosis in these cells: in fact, permissiveness to HSV-1 raises when apoptosis further increases due to NF-κB inhibition. This latter result is not surprising given the fact that NF-κB positively regulates the transcription of anti-apoptotic genes. 33 Moreover, NF-κB-dependent constriction of the apoptotic response to HSV-1 infection in monocytic cells could be beneficial for the host to limit the damage of an excessive, consequent state of immune suppression. Our data from measuring apoptosis and virus replication at the single cell level allowed us to explain the paradox represented by the simultaneous increase of virus yield and apoptotic cell death in monocytic cells in which NF-κB activation was prevented. In fact, we observed that accomplishment of HSV-1 replication and apoptosis rarely occurred within the same cell, both in NF-κB-incompetent and NF-κB-competent cells. Another novel finding about the process of the apoptotic response to HSV concerns the LMP. In fact, LMP and cathepsins' activities have been previously implicated in apoptosis induced by the d-120mutant of HSV-1, 39 but in the present study we clearly demonstrate that induction of LMP is a hallmark of apoptosis induced also by wt HSV-1.
But why are our findings so important for monocytic cells? First, monocytes/macrophages play an important role in the early defence against HSV-1. 47 Second, HSV-1 diverged from its herpes virus ancestor at the same time as an ancestor of modern humans diverged from a common primate ancestor. 48 Consequently, long-term coexistence of HSV-1 with its host established a delicate, but extremely efficient balance between the host innate response and the immune evasion strategies of the virus. Our results therefore suggest a scenario in which an efficient NF-κB-dependent innate response to HSV-1 is necessary to limit virus replication as well as an apoptotic response in monocytes/macrophages, thus avoiding an irreparable damage of the cellular innate system of the host that must contribute to locally circumscribe the infection.
In conclusion, results reported in the present study indicate that the fate of HSV-1 infected monocytic cells, whether it is apoptotic cell death/survival or high/low viral replication, depends on a delicate and complex balance between prohost and pro-virus factors that occur at single cell level and demonstrate that NF-κB signalling plays a fundamental role in controlling it. These results could contribute to the understanding of the still unexplained mechanisms controlling partial restriction of HSV-1 infection that limits permissiveness to the virus of monocytic cells and of the related pathogenetic events.
Cells, virus and treatments. Human monocytic U937 cells and their stable transfectants carrying a DN murine IκBα (U937-DN-IκB) or a control vector pcDNA3.1 (U937-pcDNA) were grown and cultured as previously described. 5 HSV-1 'F' strain, originally provided by ATCC, was used in all experiments. Virus stocks were produced, titred in Vero cells and stored in aliquots at − 80°C. Monocytic cells were split 24 h before infection and then either mock infected or exposed to different MOIs of HSV-1 for 1 h at 37°C. For kinetics experiments, cells were exposed to HSV-1 also for a shorter time of 30 min. After the adsorption period the virus inoculum was replaced with RPMI 1640 containing 1% fetal bovine serum and cultures were further incubated at 37°C for the indicated times (hours p.i.). For UV inactivation, virus suspension was exposed in Petri dish for 150 s to UV light at an intensity of 30 watts from a germicidal lamp situated 10 cm above the sample. For pharmacological IκBα phosphorylation inhibition, U937 cells were pre-treated with 1 μM of Bay 11-7085 16 h before HSV-1 infection. The Bay 11-7085 concentration used was chosen on the basis of preliminary experiments performed by trypan blue exclusion to select the non-cytotoxic concentration ranges of the drug on monocytic cells. To neutralize effects of endogenous TNFα and INFα production during HSV-1 infection, cytokine-specific neutralizing antibodies to TNFα and IFNα (Chemicon/ Millipore) were added to mock and infected cells at the end of adsorption period. After 24 h of incubation at 37°C, cells were collected and analysed for gD expression and apoptosis levels.
Immunofluorescence analysis. U937-pcDNA and U937-DN-mIκB cells, either mock infected or infected with HSV-1, were collected by centrifugation and washed in phosphate-buffered saline (PBS), placed on polylysine-coated multiwell slides and fixed for 15 min in PBS containing 3% paraformaldehyde. Cells were then washed twice in PBS and incubated for 1 h at 37°C with mouse anti-gD DL6 (1:200). After washing twice in PBS, slides were incubated for 45 min at 37°C with fluorescein isothiocyanate-conjugated goat anti-mouse-IgG secondary antibody in PBS (1:300). For analysis of nuclear morphology, 1 μg/ml of Hoechst 33342 was added to the secondary antibody. Slides were washed in PBS, covered with mounting medium, visualized and photographed by fluorescence microscopy (Leitz, Wetzlar, Germany). For quantitative determinations, images from the same field were taken with green (for fluorescein isothiocyanate-labelled antibody) or blue (for Hoechst-stained nuclei) filters. Ten randomly selected fields (magnification 400 × ; 100 cells per field) were captured for each sample to count gD-positive cells (green filter) or nuclei with apoptotic morphology (blue filter). Merged images were used to simultaneously evaluate double-positive cells and the percentages were determined by counting the total number of nucleated cells in the blue filter. Representative fields were photographed using a 630 × magnification.
For gD detection by flow cytometry, we applied the same protocol of staining used for immunofluorescence microscopy analysis except that Hoechst 33342 was omitted.
Apoptosis and lysosomal membrane assays. Apoptosis was assessed by microscopy analysis of cellular (apoptotic bodies) or nuclear (chromatin condensation, nuclear fragmentation) morphology following staining with Hoechst 3342 chromatin dye, as previously described by some of us. 25 In some experiments, apoptosis was also evaluated by flow cytometry analysis of nuclei isolated from the cells following detergent treatment and stained with propidium iodide, using a method that discriminates nuclei from apoptotic, necrotic or viable cells, as previously described. 49,50 Samples were run and analysed in a BD FACSCalibur flow cytometer using the CELLQuest II software (BD).
To quantify lysosomal membrane integrity, cells were stained with 10 μM acridine orange for 15 min or with 75 nM LysoTracker Red DND 99 (Invitrogen-Molecular Probes, Paisley, UK) for 45 min at 37°C. After several PBS washes, the reduction of red or green fluorescence was measured by FACSCalibur. 51 Nuclear extracts and electro mobility shift assay (EMSA). For detecting DNA binding activity of NF-κB present in the nuclei of U937-pcDNA and U937-DN-mIκB cells after HSV-1 infection, non-radioactive EMSA was performed. Nuclear extract preparation and EMSA were carried out according to an earlier study. 11,37 Briefly, 1 × 10 7 cells were washed with cold PBS and suspended in 0.4 ml hypotonic lysis buffer A (10 mMHepes, pH 7.9, 1.5 mM MgCl 2 , 10 mMKCl, 0.5 mMDTT, 0.2 mM PMSF) for 20 min on ice and homogenized by passing through a 25-gauge needle. After centrifugation at 12 000 × g for 40 s, nuclear pellets were resuspended in 20 μl ice-cold buffer B (20 mMHepes, pH 7.9, 25% glycerol, 0.42 M NaCl, 1.5 mM MgCl 2 , 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF) and supplemented with 1x protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN, USA). Following a 20 min incubation on ice (with recurring mixing), samples were centrifuged at 12 000 × g for 5 min and supernatants containing nuclear extracts were collected, aliquoted and immediately stored at − 80°C. The LightShift chemiluminescent EMSA kit (Pierce, Rockford, IL, USA) was utilized to perform the EMSA binding reactions. Specifically, 12 μg of nuclear extracts were incubated with 10 pmoles of double-stranded biotinylated probe containing the NF-κB consensus site, and with 1 μg/μl of poly-dI-dC, to prevent unspecific reaction, in 1 × binding buffer for 20 min at room temperature. The complexes were resolved on a 5% native polyacrylamide/0.5x TBE gel and transferred to a positive charge nylon membrane (Bio-Rad, Hercules, CA, USA). After blotting, DNA was cross-linked by UV and signals from the biotin-labelled probe were detected using reagents provided in the 'LightShift chemiluminescent EMSA module' (Pierce, Rockford, IL, USA). The NIH ImageJ software (version 1.46r, Bethesda, MD, USA) was used to evaluate densitometry of scanned films from EMSA. The density value in each signal area was calculated following subtraction of background density value evaluated in an adjacent blank area of the same size. This value represents the optical density of each shift in the films. Relative density was calculated as the ratio between HSV-1 and mock-infected cells.
Western blot analysis. Pellets from 3 × 10 6 cells were resuspended in NP-40 lysis buffer (50 mM TrisHCl, ph 8.0, 150 mM NaCl, 1% NP40, and freshly added, 1 mM PMSF, 0.5 mM DTT) completed with 1x protease inhibitor cocktails (Roche Applied Science) and incubated on ice for 20 min. After centrifugation at 12 000 × g for 10 min at 4°C, the supernatant was collected and protein concentration assessed by 'DC Protein Assay' (Bio-Rad). Equal amount of protein (60 μg) was separated by 10% SDS-PAGE, transferred to nitrocellulose membrane (Bio-Rad) and visualized by reversible Ponceau Red staining for verifying transfer efficiency. After 1 h at room temperature with blocking buffer (TBS, 0.1% Tween-20 with 5% non-fat dry milk) and several washing, the membranes were incubated overnight at 4°C with primary antibodies diluted 1:1000 in TBS-Tween containing 5% bovine serum albumin antibody, subsequently washed and then incubated with corresponding horseradish peroxidase-conjugated secondary antibody (dilution 1:2000 in TBS-T 5% non-fat dry milk). Binding of antibodies was detected by chemiluminescence staining using the ECL detection kit (Pierce). The ImageJ software was utilized to evaluate densitometric values of western blots.
Determination of mRNA expression by real-time PCR. U937-pcDNA and U937-DN-IκB cells were infected with HSV-1 at an MOI of 50 PFU/cell. At 3, 6 and 24 h p.i., HSV-1-and mock-infected cells were harvested and total RNA was isolated using TRIzol reagent (Life Technologies, Rockville, MD, USA), according to the manufacturer's instructions. DNase treatment (Thermo Scientific, Waltham, MA, USA) was carried out to remove possible DNA contamination and real-time RT-PCR analysis was performed as described before. 52 Basically, first-strand cDNA synthesis using 0.25 μg of total RNA and random primers was carried out with 'high-capacity cDNA Reverse Transcription Kits' (Applied Biosystems, Carlsbad, CA, USA), according to the supplier's instruction. Equal volumes of cDNA synthesis mixture were used for amplification of transcripts with SYBR green PCR Real Master Mix (Bio-Rad). For each transcript/sample, triplicate reactions were performed on a CFX-96 Real-time system (Bio-Rad) with genespecific primers. All the primers employed were purchased from Primm (Milan, Italy) and are listed in Table1. Each run was completed with a melting curve analysis of the PCR end products to validate the specificity of amplification. Cycle threshold (Ct) values were exported into Excel worksheets for analysis of relative changes in gene expression using the 2 -ΔΔCt method. 53 All cDNA quantities were normalized to 18 S rRNA quantities 54 obtained from the same plate and the fold changes of gene transcripts for infected samples were expressed relative to their values obtained from mock samples collected at the same time.