Figure 6 | Cell Death & Disease

Figure 6

From: Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Figure 6

The opposite effects of miR-182 and FoxO1 on osteogenic differentiation. (a and b) Transfection effects of siFoxO1 was determined by qPCR and western blot. (cf) The functions of siFoxO1 on the expression of osteogenic markers of pPDLSCs were measured by Alizarin red staining, qPCR and western blot 7 or 14 days after osteogenic induction. (g and h) Runx2 and Col1 mRNA levels and Runx2 protein level in pPDLSCs were measured after being transfected with siFoxO1 under the treatment of overexpression of lncRNA-POIR or controls at 7 or 14 days after osteogenic induction. (i and j) siFoxO1 inhibits osteogenesis of hPDLSCs in vivo. hPDLSCs were mixed with HA-TCP and transplanted into the dorsal region of nude mice for 4 weeks. Then, the results were measured by H&E staining and Masson’s trichrome staining. Quantitative analysis of the new bone area determined by Image-Pro Plus 6.0 software. At least three fields were randomly selected from each transplant. Six implants were engrafted into three mice per treatment. All experiments were repeated three times. Relative expressions of mRNAs were normalized by β-actin in qPCR. Data represent mean±S.D. *P<0.05, **P<0.01 and NS, not significant. The scale bar in the micrographs represents 200 nm. Con, control; lncRNA-POIR, lentivirus for upregulating lncRNA-POIR; NB, new bone; NC, lentivirus negative control; OD, optical density; siFoxO1, FoxO1 oligo; siNC, negative control

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