Figure 5 | Cell Death & Disease

Figure 5

From: Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Figure 5

lncRNA-POIR modulated FoxO1 by regulating miR-182 (a and b) After transfection of anti-miR-182, FoxO1 expression in pPDLSCs was measured by qPCR and western blot. (c) Schematic of the miR-182 putative target site in the FoxO1 3′-UTR. (d) The luciferase reporter assay for the lncRNA-POIR in the presence of miR-182. PPDLSCs were co-transfected with miR-Control or miR-182 and wild-type FoxO1 3′-UTR or mutant FoxO1 3′-UTR luciferase constructs. Values are reported as firefly luciferase activity to Renilla luciferase activity. (eh) After transfection of lncRNA-POIR and shlncRNA-POIR in pPDLSCs, FoxO1 expression was measured by qPCR and western blot. All experiments were repeated three times. Relative expressions of FoxO1 were normalized by β-actin in qPCR. Data represent mean±S.D. *P<0.05, **P<0.01 and NS, not significant. Anti-miR-NC, siPORT reagent alone; anti-miR-182, miR-182 inhibitor; FoxO1 3′-UTR wt, FoxO1 3′-UTR wild-type; FoxO1 3′-UTR mut, FoxO1 3′-UTR-mutated type, Con, Control; lncRNA-POIR, lentivirus for upregulating lncRNA-POIR; NC, lentivirus negative control; shlncRNA-POIR, plamids for downregulating lncRNA-POIR; shNC, plasmids negative control; UTR, untranslated regions

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