Figure 2 | Cell Death & Disease

Figure 2

From: Long noncoding RNA related to periodontitis interacts with miR-182 to upregulate osteogenic differentiation in periodontal mesenchymal stem cells of periodontitis patients

Figure 2

LncRNA-POIR promotes osteogenesis of pPDLSCs. (a and b) Transfection effects of shlncRNA-POIR plasmids and lncRNA-POIR overexpression lentiviruses were determined by qPCR. (c and d) Runx2, ALP and Col1 expressions were measured by qPCR at 0 and 7 days after osteogenic induction. (eg) Osteogenic differentiations of pPDLSCs were determined by Alizarin Red S, ALP staining and ALP activity assay 7 or 14 days after osteogenic induction. (h and i) LncRNA-POIR promotes osteogenesis of pPDLSCs in vivo. pPDLSCs were mixed with HA-TCP and transplanted into the dorsal region of nude mice for 4 weeks. Then, the results were measured by H&E staining and Masson’s trichrome staining. Quantitative analysis of the new bone area determined by Image-Pro Plus 6.0 software (Media Cybernetics, Washington, USA). At least three fields were randomly selected from each transplant. Six implants were engrafted into three mice per treatment. All experiments were repeated three times. Relative expressions of mRNAs and lncRNA-POIR were normalized by β-actin in qPCR. All data are the mean±S.D. *P<0.05, **P<0.01 and NS, not significant. The scale bar in the micrographs represents 200 nm. Con, Control; diff, osteogenic induction; lncRNA-POIR, lentivirus for upregulating lncRNA-POIR; NB, new bone; NC, lentivirus negative control; OD, optical density; shNC, plasmids negative control, shlncRNA-POIR, plamids for downregulating lncRNA-POIR

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